{"_buckets": {"deposit": "2f51ce20-2270-4cc6-bf01-49454f90c8b9"}, "_deposit": {"id": "28286", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28286"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028286"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Brassica rapa\u306b\u304a\u3051\u308b\u8fd1\u4ea4\u7cfb\u7d71\u306e\u7d14\u5ea6\u691c\u5b9a\u306b\u6700\u9069\u306aDNA\u30de\u30fc\u30ab\u30fc\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "124", "bibliographicPageStart": "111", "bibliographicVolumeNumber": "66", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Developing inbred lines as parental candidates is the first step in breeding F1 hybrid cultivars of plants. It is generally considered that selfing for more than 5 or 6 generations is necessary for generating genetically identical inbred lines. DNA-marker based purity test of inbred lines is a reliable tool to confirm their genetic homogeneity, but few DNA markers, which are suitable for purity test of inbred lines, are reported in Brassica rapa L. In this study, we assessed 321 SSR primer sets to identify the DNA markers, which can detect the polymorphisms between the parental lines of F1 hybrid cultivars. The F2 plants derived from the F1 hybrid cultivar of Chinese cabbage W77 was used to identify the polymorphisms. The F1 hybrid parental genomes in the F2 population allows detecting the nucleotide sequence differences between the individuals that can be traced back to the parental inbred lines of the F1 hybrid. Finally 59 primer sets showed polymorphisms among F2 plants, indicating that these DNA markers could be useful for purity test.", "subitem_description_type": "Abstract"}, {"subitem_description": "\u89aa\u5019\u88dc\u3068\u306a\u308b\u8fd1\u4ea4\u7cfb\u7d71\u306e\u4f5c\u6210\u306f\u3001\u690d\u7269\u306e\u4e00\u4ee3\u96d1\u7a2e\uff08F1\uff09\u54c1\u7a2e\u306e\u80b2\u6210\u904e\u7a0b\u306e\u6700\u521d\u306e\u6bb5\u968e\u3067\u3042\u308b\u3002\u4e00\u822c\u7684\u306b\u907a\u4f1d\u7684\u306b\u5747\u4e00\u306a\u8fd1\u4ea4\u7cfb\u7d71\u306e\u80b2\u6210\u306b\u306f\uff15\u3001\uff16\u4e16\u4ee3\u4ee5\u4e0a\u306e\u81ea\u6b96\u304c\u5fc5\u8981\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u3002DNA\u30de\u30fc\u30ab\u30fc\u3092\u5229\u7528\u3057\u305f\u8fd1\u4ea4\u7cfb\u306e\u7d14\u5ea6\u691c\u5b9a\u306f\u4fe1\u983c\u6027\u304c\u9ad8\u3044\u304c\u3001Brassica rapa L. 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[{"creatorName": "Kawamura, Kazutaka"}], "nameIdentifiers": [{"nameIdentifier": "162514", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saeki, Natsumi"}], "nameIdentifiers": [{"nameIdentifier": "162515", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ebe, Yusuke"}], "nameIdentifiers": [{"nameIdentifier": "162516", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Abe, Hirofumi"}], "nameIdentifiers": [{"nameIdentifier": "162517", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimizu, Motoki"}], "nameIdentifiers": [{"nameIdentifier": "162518", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Osabe, Kenji"}], "nameIdentifiers": [{"nameIdentifier": "162519", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okazaki, Keiichi"}], "nameIdentifiers": [{"nameIdentifier": "162520", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawanabe, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "162521", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimoto, Ryo"}], "nameIdentifiers": [{"nameIdentifier": "162522", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "66(2)_111-124.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1200000.0, "url": {"label": "66(2)_111-124.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28286/files/66(2)_111-124.pdf"}, "version_id": "9501d928-be8c-4751-9266-11a0da38e990"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Purity test", "subitem_subject_scheme": "Other"}, {"subitem_subject": "inbred line", "subitem_subject_scheme": "Other"}, {"subitem_subject": "simple sequence repeats (SSR) primers", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brassica rapa", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u7d14\u5ea6\u691c\u5b9a", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u8fd1\u4ea4\u7cfb", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SSR\u30de\u30fc\u30ab\u30fc", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa"}, {"subitem_title": "Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1589"], "permalink_uri": "http://hdl.handle.net/10191/26810", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2014-04-07"}, "publish_date": "2014-04-07", "publish_status": "0", "recid": "28286", "relation": {}, "relation_version_is_last": true, "title": ["Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa"], "weko_shared_id": null}