{"created":"2021-03-01T06:34:04.727970+00:00","id":28286,"links":{},"metadata":{"_buckets":{"deposit":"2f51ce20-2270-4cc6-bf01-49454f90c8b9"},"_deposit":{"id":"28286","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28286"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028286","sets":["453:456","485:872:1583:1589"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Brassica rapaにおける近交系統の純度検定に最適なDNAマーカーのスクリーニング"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"124","bibliographicPageStart":"111","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Developing inbred lines as parental candidates is the first step in breeding F1 hybrid cultivars of plants. It is generally considered that selfing for more than 5 or 6 generations is necessary for generating genetically identical inbred lines. DNA-marker based purity test of inbred lines is a reliable tool to confirm their genetic homogeneity, but few DNA markers, which are suitable for purity test of inbred lines, are reported in Brassica rapa L. In this study, we assessed 321 SSR primer sets to identify the DNA markers, which can detect the polymorphisms between the parental lines of F1 hybrid cultivars. The F2 plants derived from the F1 hybrid cultivar of Chinese cabbage W77 was used to identify the polymorphisms. The F1 hybrid parental genomes in the F2 population allows detecting the nucleotide sequence differences between the individuals that can be traced back to the parental inbred lines of the F1 hybrid. Finally 59 primer sets showed polymorphisms among F2 plants, indicating that these DNA markers could be useful for purity test.","subitem_description_type":"Abstract"},{"subitem_description":"親候補となる近交系統の作成は、植物の一代雑種（F1）品種の育成過程の最初の段階である。一般的に遺伝的に均一な近交系統の育成には５、６世代以上の自殖が必要であると考えられている。DNAマーカーを利用した近交系の純度検定は信頼性が高いが、Brassica rapa L. では、純度検定に有効なDNAマーカーの報告は少ない。本研究では、F1品種の両親系統間の多型を見分けることができるDNAマーカーを見出すために、321のSSR プライマー対を試した。F2集団では、両親系統のゲノムが分離することから、両親系統の塩基配列の多型を追跡することが可能となる。材料にはハクサイの市販F1品種であるW77のF2個体を用いた。59のDNAマーカーでF2集団の個体間で多型が見られたことから、これらのDNAマーカーは純度検定に有効的であると考えられた。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"162523","nameIdentifierScheme":"WEKO"}],"names":[{"name":"川村, 和隆"}]},{"nameIdentifiers":[{"nameIdentifier":"162524","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐伯, なつみ"}]},{"nameIdentifiers":[{"nameIdentifier":"162525","nameIdentifierScheme":"WEKO"}],"names":[{"name":"江部, 裕介"}]},{"nameIdentifiers":[{"nameIdentifier":"162526","nameIdentifierScheme":"WEKO"}],"names":[{"name":"阿部, 寛史"}]},{"nameIdentifiers":[{"nameIdentifier":"162527","nameIdentifierScheme":"WEKO"}],"names":[{"name":"清水, 元樹"}]},{"nameIdentifiers":[{"nameIdentifier":"162528","nameIdentifierScheme":"WEKO"}],"names":[{"name":"長部, 謙二"}]},{"nameIdentifiers":[{"nameIdentifier":"162529","nameIdentifierScheme":"WEKO"}],"names":[{"name":"岡崎, 桂一"}]},{"nameIdentifiers":[{"nameIdentifier":"162530","nameIdentifierScheme":"WEKO"}],"names":[{"name":"川辺, 隆大"}]},{"nameIdentifiers":[{"nameIdentifier":"162531","nameIdentifierScheme":"WEKO"}],"names":[{"name":"藤本, 龍"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawamura, Kazutaka"}],"nameIdentifiers":[{"nameIdentifier":"162514","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saeki, Natsumi"}],"nameIdentifiers":[{"nameIdentifier":"162515","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ebe, Yusuke"}],"nameIdentifiers":[{"nameIdentifier":"162516","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Hirofumi"}],"nameIdentifiers":[{"nameIdentifier":"162517","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Motoki"}],"nameIdentifiers":[{"nameIdentifier":"162518","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Osabe, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"162519","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okazaki, Keiichi"}],"nameIdentifiers":[{"nameIdentifier":"162520","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawanabe, Takahiro"}],"nameIdentifiers":[{"nameIdentifier":"162521","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimoto, Ryo"}],"nameIdentifiers":[{"nameIdentifier":"162522","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"66(2)_111-124.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"66(2)_111-124.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28286/files/66(2)_111-124.pdf"},"version_id":"9501d928-be8c-4751-9266-11a0da38e990"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Purity test","subitem_subject_scheme":"Other"},{"subitem_subject":"inbred line","subitem_subject_scheme":"Other"},{"subitem_subject":"simple sequence repeats (SSR) primers","subitem_subject_scheme":"Other"},{"subitem_subject":"Brassica rapa","subitem_subject_scheme":"Other"},{"subitem_subject":"純度検定","subitem_subject_scheme":"Other"},{"subitem_subject":"近交系","subitem_subject_scheme":"Other"},{"subitem_subject":"SSRマーカー","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa"},{"subitem_title":"Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1589"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-04-07"},"publish_date":"2014-04-07","publish_status":"0","recid":"28286","relation_version_is_last":true,"title":["Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:13.405472+00:00"}