{"_buckets": {"deposit": "8cc3b1c8-b72d-409d-875d-81a3620ca6b8"}, "_deposit": {"id": "28276", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28276"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028276"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u5c0e\u7ba1\u6db2\u3068\u830e\u30a2\u30dd\u30d7\u30e9\u30b9\u30c8\u6db2\u306e\u5fae\u91cf\u76f8\u5bfe\u30a6\u30ec\u30a4\u30c9\u5206\u6790\u306b\u3088\u308b\u30c0\u30a4\u30ba\u306e\u7a92\u7d20\u56fa\u5b9a\u4f9d\u5b58\u7387\u306e\u63a8\u5b9a"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "41", "bibliographicPageStart": "27", "bibliographicVolumeNumber": "67", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Relative ureide method is a simple and reliable method for estimating the percentage of nitrogen depending on nitrogen fixation in field grown soybean plants. Generally, the concentrations of ureides, amides and nitrate in root bleeding xylem sap from a cut lower main stem are determined colorimetrically using an optical spectrometer. We developed a micro-scale analysis of these components in xylem sap using 2.5 \u03bcL of xylem sap for each analysis instead of 50 \u03bcL for the standard assays. Each reaction was carried out in a 1.5 mL Eppendorf centrifuge tube, and the 200 \u03bcL of reaction mixture was put into a well of a 96 wells-microplate, and measured by a microplate reader. The duplicated analysis of the standard solution gave accurate calibration for each assay. We can analyze about 200 samples in one day by the micro-scale analysis. In addition, we tried to compare the stem fluid collected by centrifuge of the lower stem segments, because root bleeding sap cannot be collected under dry conditions or at late stages such as R7 stage. Stem fluid was always collected by a portable centrifuge at 2,200 x G for 10 seconds, and the volume increased from R1 to R7 stages. The concentrations of nitrogen components and the relative ureide percent in root bleeding xylem xap and stem fluid were similar at R7, but different at R1 and R5 stages. The relative ureide percentages from stem fluid were about 50% at R1 and R5, but those from xylem sap were about 80-90%. Therefore, the stem fluid may be used for relative ureide percent only at R7 stage, when xylem sap is sometimes difficult to obtain.", "subitem_description_type": "Abstract"}, {"subitem_description": "\u76f8\u5bfe\u30a6\u30ec\u30a4\u30c9\u6cd5\u306f\u3001\u5703\u5834\u683d\u57f9\u30c0\u30a4\u30ba\u306e\u7a92\u7d20\u56fa\u5b9a\u4f9d\u5b58\u7387\u3092\u63a8\u5b9a\u3059\u308b\u305f\u3081\u306e\u7c21\u4fbf\u3067\u4fe1\u983c\u306e\u3067\u304d\u308b\u65b9\u6cd5\u3067\u3042\u308b\u3002\u4e00\u822c\u306b\u4e3b\u830e\u682a\u5207\u65ad\u9762\u304b\u3089\u6ea2\u6ccc\u3059\u308b\u5c0e\u7ba1\u6db2\u4e2d\u306e\u785d\u9178\u3001\u30a2\u30df\u30c9\u3001\u30a6\u30ec\u30a4\u30c9\u6fc3\u5ea6\u3092\u5206\u5149\u5149\u5ea6\u8a08\u3092\u7528\u3044\u3066\u6bd4\u8272\u5b9a\u91cf\u3059\u308b\u3002\u6211\u3005\u306f\u3001\u901a\u5e38\u306e\u5206\u6790\u3067\u7528\u3044\u308b\u5c0e\u7ba1\u6db2\u91cf50 \u03bcL \u306e\u4ee3\u308f\u308a\u306b\u3001\u305d\u306e1/20\u306e2.5 \u03bcL \u3092\u7528\u3044\u308b\u5fae\u91cf\u5206\u6790\u6cd5\u3092\u958b\u767a\u3057\u305f\u3002\u5448\u8272\u53cd\u5fdc\u306f\u30011.5 mL \u30a8\u30c3\u30da\u30f3\u30c9\u30eb\u30d5\u30c1\u30e5\u30fc\u30d6\u5185\u3067\u884c\u306a\u3044\u3001\u5448\u8272\u6db2200 \u03bcL \u309296\u7a74\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u306e\u30a6\u30a7\u30eb\u306b\u5165\u308c\u3001\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\u3067\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u305f\u3002\u6a19\u6e96\u6db2\u3092\uff12\u9023\u3067\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u7cbe\u5ea6\u306e\u9ad8\u3044\u691c\u91cf\u7dda\u306e\u4f5c\u6210\u304c\u53ef\u80fd\u3067\u3042\u3063\u305f\u3002\u672c\u5206\u6790\u6cd5\u3092\u7528\u3044\u308b\u3053\u3068\u306b\u3088\u308a\u30011\u65e5\u306b200\u8a66\u6599\u3092\u6e2c\u5b9a\u3067\u304d\u305f\u3002\u307e\u305f\u3001\u751f\u80b2\u5f8c\u671f\uff08\u9ec4\u8449\u671fR7\uff09\u3084\u3001\u571f\u58cc\u304c\u4e7e\u71e5\u3059\u308b\u3068\u3001\u5c0e\u7ba1\u6db2\u3092\u6ea2\u6ccc\u3057\u306a\u3044\u3053\u3068\u304c\u3042\u308b\u305f\u3081\u3001\u78ba\u5b9f\u306b\u8a66\u6599\u3092\u63a1\u53d6\u3059\u308b\u65b9\u6cd5\u3068\u3057\u3066\u3001\u643a\u5e2f\u578b\u9060\u5fc3\u5668\uff082,200 x 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"item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Relative ureide method", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Soybean", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Microplate", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Xylem sap", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Stem fluid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u76f8\u5bfe\u30a6\u30ec\u30a4\u30c9\u6cd5", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30c0\u30a4\u30ba", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u5c0e\u7ba1\u6db2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30a2\u30dd\u30d7\u30e9\u30b9\u30c8\u6db2", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Estimation of nitrogen fixation rate of soybean (Glycine max (L.) Merr.) by micro-scale relative ureide analysis using root bleeding xylem sap and apoplast fluid in stem.", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Estimation of nitrogen fixation rate of soybean (Glycine max (L.) Merr.) by micro-scale relative ureide analysis using root bleeding xylem sap and apoplast fluid in stem."}, {"subitem_title": "Estimation of nitrogen fixation rate of soybean (Glycine max (L.) Merr.) by micro-scale relative ureide analysis using root bleeding xylem sap and apoplast fluid in stem.", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1588"], "permalink_uri": "http://hdl.handle.net/10191/30053", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2014-10-06"}, "publish_date": "2014-10-06", "publish_status": "0", "recid": "28276", "relation": {}, "relation_version_is_last": true, "title": ["Estimation of nitrogen fixation rate of soybean (Glycine max (L.) Merr.) by micro-scale relative ureide analysis using root bleeding xylem sap and apoplast fluid in stem."], "weko_shared_id": null}