{"_buckets": {"deposit": "a58afef0-e347-473f-9d4f-b2a1524699c1"}, "_deposit": {"id": "2778", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2778"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002778"}, "item_5_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30e6\u30ad\u30ef\u30ea\u30bd\u30a6\uff08Hepatica nobilis Schreber var. japonica f. magna\uff09\u306e\u8449\u7247\u57f9\u990a\u306b\u3088\u308b\u6804\u990a\u7e41\u6b96"}]}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "211", "bibliographicPageStart": "205", "bibliographicVolumeNumber": "72", "bibliographic_titles": [{"bibliographic_title": "\u5712\u82b8\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u5712\u82b8\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "\u30e6\u30ad\u30ef\u30ea\u30bd\u30a6\uff08Hepatica nobilis\uff09\u306e\u7d44\u7e54\u57f9\u990a\u6cd5\u306b\u3088\u308b\u6804\u990a\u7e41\u6b96\u6cd5\u3092\u7814\u7a76\u3057\u305f\uff0e\u958b\u82b1\u3057\u305f\u690d\u7269\u304b\u3089\u5f53\u5e74\u306b\u6210\u9577\u3057\u305f\u8449\u3092\u63a1\u96c6\u3057\uff0c5\u00d75mm\u306e\u5207\u7247\u3092\u4f5c\u308a\u57f9\u990a\u3057\u305f\uff0eNAA\u3068BA\u3092\u542b\u3080MS\u57f9\u5730\u3067\u306f\u305d\u306e\u6fc3\u5ea6\u3068\u7121\u95a2\u4fc2\u306b\u3044\u305a\u308c\u306e\u57f9\u5730\u3067\u3082\u9ad8\u983b\u5ea6\u3067\u30ab\u30eb\u30b9\u3092\u5f62\u6210\u3057\u305f\uff0e\u4e0d\u5b9a\u82bd\u306e\u5f62\u6210\u7387\u306fNAA0.5\u307e\u305f\u306f1mg\u30fbliter^-1\u3068BA5mg\u30fbliter^-1\u3092\u6dfb\u52a0\u3057\u305f\u57f9\u5730\u3067\u6700\u3082\u9ad8\u304f\u306a\u3063\u305f\uff0e\u4e00\u65b9\uff0c\u80da\u69d8\u4f53\u306e\u5f62\u6210\u306fNAA\u30920.1mg\u30fbliter^-1\u3068BA\u30920.5mg\u30fbliter^-1\u307e\u305f\u306fNAA\u3068BA\u30921mg\u30fbliter^-1\u305a\u3064\u6dfb\u52a0\u3057\u305f\u57f9\u5730\u3067\u4fc3\u9032\u3055\u308c\u305f\uff0e\u8449\u5207\u7247\u306b\u5f62\u6210\u3055\u308c\u305f\u9577\u30551-2mm\u306e\u4e0d\u5b9a\u82bd\uff081\u6b21\u4e0d\u5b9a\u82bd\u3068\u547c\u3076\uff09\u3092\u5207\u308a\u53d6\u308a\uff0cBA\u3068GA_3\u3092\u542b\u3080MS\u57f9\u5730\u30678\u9031\u9593\u6bce\u306b\u7d99\u4ee3\u3057\uff0c24\u9031\u9593\u57f9\u990a\u3057\u305f\uff0e1\u6b21\u4e0d\u5b9a\u82bd\u306e\u751f\u5b58\u7387\u306fBA\u3092\u5358\u72ec\u3067\u6dfb\u52a0\u3057\u305f\u3068\u304d\u3088\u308a\u3082BA\u3068GA_3\u3092\u6dfb\u52a0\u3057\u305f\u6642\u306b\u9ad8\u304f\u306a\u3063\u305f\uff0e\u305d\u3057\u3066\u4e0d\u5b9a\u82bd\u306e\u6210\u9577\u306fBA5mg\u30fbliter^-1\u3068GA_3 10mg\u30fbliter^-1\u3092\u6dfb\u52a0\u3057\u305f\u57f9\u5730\u3067\u4fc3\u9032\u3055\u308c\uff0c\u305d\u306e\u57fa\u90e8\u306b\u65b0\u82bd\uff082\u6b21\u4e0d\u5b9a\u82bd\u3068\u547c\u3076\uff09\u3092\u591a\u304f\u5f62\u6210\u3057\u305f\uff0e\u9577\u305510-20mm\u306b\u6210\u9577\u3057\u305f2\u6b21\u4e0d\u5b9a\u82bd\u3092\u5207\u308a\u53d6\u308a\uff0c10mg\u30fbliter^-1\u306eNAA\u307e\u305f\u306fIBA\u3092\u542b\u3080MS\u57f9\u5730\uff0c\u6697\u9ed2\u4e0b\u30671\u9031\u9593\u57f9\u990a\u3057\uff0c\u305d\u306e\u5f8c\u660e\u6761\u4ef6\u4e0b\uff0c\u30db\u30eb\u30e2\u30f3\u30d5\u30ea\u30fc\u57f9\u5730\u3067\u57f9\u990a\u3059\u308b\u3068\uff0c\u30b7\u30e5\u30fc\u30c8\u306f\u9ad8\u983b\u5ea6\u3067\u767a\u6839\u3057\u305f\uff0e\u4e00\u65b9\uff0cNAA\u307e\u305f\u306fIBA\u3092\u542b\u3080\u57f9\u5730\u306b\u7d99\u4ee3\u3057\u305f\u3068\u304d\u306f\uff0c\u57fa\u90e8\u306b\u30ab\u30eb\u30b9\u3092\u5f62\u6210\u3057\uff0c\u767a\u6839\u3057\u306a\u304b\u3063\u305f\uff0e\u767a\u6839\u3057\u305f\u30b7\u30e5\u30fc\u30c8\u306f\u7d0425\u2103\u306e\u9ad8\u6e7f\u5ea6\u6761\u4ef6\u4e0b\u3067\u7d041\u30f6\u6708\u9593\u9806\u5316\u3055\u305b\uff0c\u7121\u52a0\u6e29\u306e\u30ac\u30e9\u30b9\u5ba4\u306b\u79fb\u3057\u3066\u683d\u57f9\u3059\u308b\u3068\uff0c\u79fb\u690d\u3057\u305f\u82d7\u306f\u307b\u3068\u3093\u3069\u3059\u3079\u3066\u751f\u5b58\u3057\uff0c2\u5e74\u4ee5\u5185\u306b\u958b\u82b1\u3057\u305f\uff0e", "subitem_description_type": "Abstract"}, {"subitem_description": "This study was conducted to establish a system of in vitro micropropagation of Hepatica nobilis. Leaf segments, excised from commercial plants, formed calli at high frequency in MS medium that contained 0.1-10mg\u30fbliter^-1 NAA and BA. The highest percentage of bud formation was observed to be about 20% in the medium supplemented with 0.5 or 1mg\u30fbliter^-1 NAA and 5mg\u30fbliter^-1 BA. Embryoid formation was stimulated on the medium containing 0.1mg\u30fbliter^-1 NAA and 0.5mg\u30fbliter^-1 BA, or 1mg\u30fbliter^-1 NAA and BA. When 1 to 2mm long adventitious buds were excised from leaf segments and recultured every 8 weeks on MS medium supplemented with BA, GA_3 or both for 24 weeks, they developed into shoots (referred to as the primary shoots in this text). The survival rates of the primary shoots were higher in the medium containing both BA and GA_3 than in the medium with BA alone. When primary shoots were cultured on MS medium containing 5mg\u30fbliter BA and 10mg\u30fbliter^-1 GAs, they formed the highest number of new shoots, referred to as secondary shoots in the text. When excised secondary shoots were cultured in MS medium containing 10mg\u30fbliter^-1 NAA or IBA in the dark for a week and then transplanted into MS-medium without any hormones, they developed roots at a high frequency, whereas shoots transplanted to MS-medium with NAA or IBA formed only callus at the base. Following acclimatization, rooted plants that were transplanted into pots and grown in a glasshouse, flowered within 2 years.", "subitem_description_type": "Abstract"}]}, "item_5_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "38994", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u91ce\u6c34, \u5229\u548c"}]}, {"nameIdentifiers": [{"nameIdentifier": "38995", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u65b0\u7f8e, \u82b3\u4e8c"}]}, {"nameIdentifiers": [{"nameIdentifier": "38996", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u7b20\u539f, \u4fca\u7b56"}]}]}, "item_5_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u5712\u82b8\u5b66\u4f1a"}]}, "item_5_rights_15": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u5712\u82b8\u5b66\u4f1a"}]}, "item_5_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00025257", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00137626", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nomizu, Toshikazu"}], "nameIdentifiers": [{"nameIdentifier": "38991", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Niimi, Yoshiji"}], "nameIdentifiers": [{"nameIdentifier": "38992", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kasahara, Shunsaku"}], "nameIdentifiers": [{"nameIdentifier": "38993", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "72(3)_205-211.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "72(3)_205-211.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/2778/files/72(3)_205-211.pdf"}, "version_id": "19157129-f5a7-4c9b-b0e9-37c4392c1002"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "adventitious bud", "subitem_subject_scheme": "Other"}, {"subitem_subject": "flowering", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rooting", "subitem_subject_scheme": "Other"}, {"subitem_subject": "shoot", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "In Vitro Micropropagation of \u2018Yukiwariso\u2019(Hepatica nobilis Schreber var, japonica f. magna) by Leaf Segment Culture", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "In Vitro Micropropagation of \u2018Yukiwariso\u2019(Hepatica nobilis Schreber var, japonica f. magna) by Leaf Segment Culture"}, {"subitem_title": "In Vitro Micropropagation of \u2018Yukiwariso\u2019(Hepatica nobilis Schreber var, japonica f. magna) by Leaf Segment Culture", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["453/454", "485/486/487"], "permalink_uri": "http://hdl.handle.net/10191/6578", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2008-10-15"}, "publish_date": "2008-10-15", "publish_status": "0", "recid": "2778", "relation": {}, "relation_version_is_last": true, "title": ["In Vitro Micropropagation of \u2018Yukiwariso\u2019(Hepatica nobilis Schreber var, japonica f. magna) by Leaf Segment Culture"], "weko_shared_id": null}