ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "11e3b5b0-fa79-4443-9aea-c2b87d9e956e"}, "_deposit": {"id": "2777", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2777"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002777", "sets": ["454", "470"]}, "author_link": ["38977", "38978", "38979", "38980", "38981", "38982", "38983", "38984", "38985", "38986", "38987", "38988", "38989", "38990"], "control_number": "2777", "item_1627363077551": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_5_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "アグロバクテリウム法によるムスカリの形質転換におけるbar遺伝子とhpt遺伝子の選抜効率の比較", "subitem_alternative_title_language": "ja"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "62", "bibliographicPageStart": "60", "bibliographicVolumeNumber": "74", "bibliographic_titles": [{"bibliographic_title": "園芸学会雑誌", "bibliographic_titleLang": "ja"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Embryogenic calli of Muscari armeniacum cv. Blue Pearl were co-cultivated with Agrobac- terium tumefaciens EHA101/pBH, which harbored a binary vector that carries the phosphi- nothricin acetyltransferase (bar) and hygromycin phosphotransferase (hpf) genes. The calli were then transferred onto the selection media containing either 4 mg-liter^\u003c-1\u003e bialaphos or 75 mg- liter\"1 hygromycin. Four to five weeks after transfer to the selection media, both bialaphos- resistant (Bia^r) and hygromycin-resistance (Hyg^r) cell clusters were produced. Over 90% of callus lines selected on a bialaphos-containing medium were verified to be transgenic by PCR analysis; this selection efficiency is comparable to that based on the hpt gene. Leaf segments of plantlets regenerated from the transgenic Bia^r callus lines showed resistance to 4 mg ・ liter^\u003c-1\u003e of bialaphos, indicating that the bar gene is useful not only as a selectable marker but also as a producer of herbicide-resistant transgenic plants of M. armeniacum.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}, {"subitem_description": "アグロバクテリウム法によるムスカリ(Muscari armericum cv. Blue pearl)の形質転換における選抜マーカー遺伝子barの有効性を調査した.葉片由来のエンブリオジエニックカルスをA.tumefaciens EHA101 / pBH(ビアラホス耐性遺伝子barおよびハイグロマイシン耐性遺伝子hptがT-DNA上にコードされている)との共存培養後,4mg・liter^\u003c-1\u003eビアラホスまたは75 mg・liter^\u003c-1\u003eハイグロマイシンを含む選抜培地に移植した.選抜開始4〜5週間後,ビアラホス耐性またはハイグロマイシン耐性カルスがそれぞれの選抜培地上で形成された. PCR分析の結果,陥r遺伝子を用いた場合の形質転換効率は90%以上となり,この効率は知z遺伝子を用いた場合の選抜効率と同等であった.また,ビアラホス耐性形質転換カルスから再生した植物体の葉片は4 mg・liter^\u003c-1\u003eビアラホスに耐性を示した.これらの結果より,アグロバクテリウム法によるムスカリの形質転換において,bar遺伝子は有効な選抜マーカーとして利用できるだけでなく,除草剤耐性形質転換体の作出にも利用可能なことが明らかとなった.", "subitem_description_language": "ja", "subitem_description_type": "Abstract"}]}, "item_5_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "38984", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "鈴木, 栄", "nameLang": "ja"}]}, {"nameIdentifiers": [{"nameIdentifier": "38985", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "中野, 優", "nameLang": "ja"}]}, {"nameIdentifiers": [{"nameIdentifier": "38986", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "小池, 洋介", "nameLang": "ja"}]}, {"nameIdentifiers": [{"nameIdentifier": "38987", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "上田, 健治", "nameLang": "ja"}]}, {"nameIdentifiers": [{"nameIdentifier": "38988", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "井上, 正保", "nameLang": "ja"}]}, {"nameIdentifiers": [{"nameIdentifier": "38989", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "西原, 昌宏", "nameLang": "ja"}]}, {"nameIdentifiers": [{"nameIdentifier": "38990", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "山村, 三郎", "nameLang": "ja"}]}]}, "item_5_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "園芸学会", "subitem_publisher_language": "ja"}]}, "item_5_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.2503/jjshs.74.", "subitem_relation_type_select": "DOI"}}]}, "item_5_relation_31": {"attribute_name": "異版である", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://ci.nii.ac.jp/naid/10014270822", "subitem_relation_type_select": "URI"}}]}, "item_5_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "園芸学会"}, {"subitem_rights": "本文データは学協会の許諾に基づきCiNiiから複製したものである"}]}, "item_5_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00025257", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0013-7626", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Suzuki, Sakae", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38977", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakano, Masaru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38978", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Koike, Yosuke", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38979", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ueda, Kenji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38980", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inoue, Masayasu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38981", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishihara, Masahiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38982", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamura, Saburo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38983", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "10014270822.pdf", "filesize": [{"value": "342.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 342700.0, "url": {"label": "10014270822.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/2777/files/10014270822.pdf"}, "version_id": "8a912a24-a971-4c17-82de-c65bf0ecfef5"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Agrobacterium tumefaciens", "subitem_subject_scheme": "Other"}, {"subitem_subject": "bar gene", "subitem_subject_scheme": "Other"}, {"subitem_subject": "herbicide-resistant transgenic plants", "subitem_subject_scheme": "Other"}, {"subitem_subject": "hpt gene", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Muscari armeniacum", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["454", "470"], "permalink_uri": "http://hdl.handle.net/10191/24666", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-01-08"}, "publish_date": "2014-01-08", "publish_status": "0", "recid": "2777", "relation": {}, "relation_version_is_last": true, "title": ["Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum"], "weko_shared_id": -1}
  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 230 大学院自然科学研究科
  2. 10 学術雑誌論文
  3. 10 査読済論文

Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum

http://hdl.handle.net/10191/24666
http://hdl.handle.net/10191/24666
cb2ceb51-4d5b-48e5-924d-1b789347f79d
名前 / ファイル ライセンス アクション
10014270822.pdf 10014270822.pdf (342.7 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-01-08
タイトル
言語 en
タイトル Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum
言語
言語 eng
キーワード
主題Scheme Other
主題 Agrobacterium tumefaciens
キーワード
主題Scheme Other
主題 bar gene
キーワード
主題Scheme Other
主題 herbicide-resistant transgenic plants
キーワード
主題Scheme Other
主題 hpt gene
キーワード
主題Scheme Other
主題 Muscari armeniacum
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
その他のタイトル
その他のタイトル アグロバクテリウム法によるムスカリの形質転換におけるbar遺伝子とhpt遺伝子の選抜効率の比較
言語 ja
著者 Suzuki, Sakae

× Suzuki, Sakae

WEKO 38977

en Suzuki, Sakae

Search repository
Nakano, Masaru

× Nakano, Masaru

WEKO 38978

en Nakano, Masaru

Search repository
Koike, Yosuke

× Koike, Yosuke

WEKO 38979

en Koike, Yosuke

Search repository
Ueda, Kenji

× Ueda, Kenji

WEKO 38980

en Ueda, Kenji

Search repository
Inoue, Masayasu

× Inoue, Masayasu

WEKO 38981

en Inoue, Masayasu

Search repository
Nishihara, Masahiro

× Nishihara, Masahiro

WEKO 38982

en Nishihara, Masahiro

Search repository
Yamamura, Saburo

× Yamamura, Saburo

WEKO 38983

en Yamamura, Saburo

Search repository
著者別名
識別子 38984
識別子Scheme WEKO
姓名 鈴木, 栄
言語 ja
著者別名
識別子 38985
識別子Scheme WEKO
姓名 中野, 優
言語 ja
著者別名
識別子 38986
識別子Scheme WEKO
姓名 小池, 洋介
言語 ja
著者別名
識別子 38987
識別子Scheme WEKO
姓名 上田, 健治
言語 ja
著者別名
識別子 38988
識別子Scheme WEKO
姓名 井上, 正保
言語 ja
著者別名
識別子 38989
識別子Scheme WEKO
姓名 西原, 昌宏
言語 ja
著者別名
識別子 38990
識別子Scheme WEKO
姓名 山村, 三郎
言語 ja
抄録
内容記述タイプ Abstract
内容記述 Embryogenic calli of Muscari armeniacum cv. Blue Pearl were co-cultivated with Agrobac- terium tumefaciens EHA101/pBH, which harbored a binary vector that carries the phosphi- nothricin acetyltransferase (bar) and hygromycin phosphotransferase (hpf) genes. The calli were then transferred onto the selection media containing either 4 mg-liter^<-1> bialaphos or 75 mg- liter"1 hygromycin. Four to five weeks after transfer to the selection media, both bialaphos- resistant (Bia^r) and hygromycin-resistance (Hyg^r) cell clusters were produced. Over 90% of callus lines selected on a bialaphos-containing medium were verified to be transgenic by PCR analysis; this selection efficiency is comparable to that based on the hpt gene. Leaf segments of plantlets regenerated from the transgenic Bia^r callus lines showed resistance to 4 mg ・ liter^<-1> of bialaphos, indicating that the bar gene is useful not only as a selectable marker but also as a producer of herbicide-resistant transgenic plants of M. armeniacum.
言語 en
抄録
内容記述タイプ Abstract
内容記述 アグロバクテリウム法によるムスカリ(Muscari armericum cv. Blue pearl)の形質転換における選抜マーカー遺伝子barの有効性を調査した.葉片由来のエンブリオジエニックカルスをA.tumefaciens EHA101 / pBH(ビアラホス耐性遺伝子barおよびハイグロマイシン耐性遺伝子hptがT-DNA上にコードされている)との共存培養後,4mg・liter^<-1>ビアラホスまたは75 mg・liter^<-1>ハイグロマイシンを含む選抜培地に移植した.選抜開始4〜5週間後,ビアラホス耐性またはハイグロマイシン耐性カルスがそれぞれの選抜培地上で形成された. PCR分析の結果,陥r遺伝子を用いた場合の形質転換効率は90%以上となり,この効率は知z遺伝子を用いた場合の選抜効率と同等であった.また,ビアラホス耐性形質転換カルスから再生した植物体の葉片は4 mg・liter^<-1>ビアラホスに耐性を示した.これらの結果より,アグロバクテリウム法によるムスカリの形質転換において,bar遺伝子は有効な選抜マーカーとして利用できるだけでなく,除草剤耐性形質転換体の作出にも利用可能なことが明らかとなった.
言語 ja
書誌情報 ja : 園芸学会雑誌

巻 74, 号 1, p. 60-62, 発行日 2005-01
出版者
言語 ja
出版者 園芸学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 0013-7626
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00025257
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.2503/jjshs.74.
権利
権利情報 園芸学会
権利
権利情報 本文データは学協会の許諾に基づきCiNiiから複製したものである
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
異版である
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://ci.nii.ac.jp/naid/10014270822
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 20:25:12.831028
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3