{"_buckets": {"deposit": "561b9e9d-bf55-4c6f-bd72-3984cba659b1"}, "_deposit": {"id": "2777", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2777"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002777"}, "item_5_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30a2\u30b0\u30ed\u30d0\u30af\u30c6\u30ea\u30a6\u30e0\u6cd5\u306b\u3088\u308b\u30e0\u30b9\u30ab\u30ea\u306e\u5f62\u8cea\u8ee2\u63db\u306b\u304a\u3051\u308bbar\u907a\u4f1d\u5b50\u3068hpt\u907a\u4f1d\u5b50\u306e\u9078\u629c\u52b9\u7387\u306e\u6bd4\u8f03"}]}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "62", "bibliographicPageStart": "60", "bibliographicVolumeNumber": "74", "bibliographic_titles": [{"bibliographic_title": "\u5712\u82b8\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u5712\u82b8\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Embryogenic calli of Muscari armeniacum cv. Blue Pearl were co-cultivated with Agrobac- terium tumefaciens EHA101/pBH, which harbored a binary vector that carries the phosphi- nothricin acetyltransferase (bar) and hygromycin phosphotransferase (hpf) genes. The calli were then transferred onto the selection media containing either 4 mg-liter^\u003c-1\u003e bialaphos or 75 mg- liter\"1 hygromycin. Four to five weeks after transfer to the selection media, both bialaphos- resistant (Bia^r) and hygromycin-resistance (Hyg^r) cell clusters were produced. Over 90% of callus lines selected on a bialaphos-containing medium were verified to be transgenic by PCR analysis; this selection efficiency is comparable to that based on the hpt gene. Leaf segments of plantlets regenerated from the transgenic Bia^r callus lines showed resistance to 4 mg \u30fb liter^\u003c-1\u003e of bialaphos, indicating that the bar gene is useful not only as a selectable marker but also as a producer of herbicide-resistant transgenic plants of M. armeniacum.", "subitem_description_type": "Abstract"}, {"subitem_description": "\u30a2\u30b0\u30ed\u30d0\u30af\u30c6\u30ea\u30a6\u30e0\u6cd5\u306b\u3088\u308b\u30e0\u30b9\u30ab\u30ea(Muscari armericum cv. 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{"subitem_subject": "herbicide-resistant transgenic plants", "subitem_subject_scheme": "Other"}, {"subitem_subject": "hpt gene", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Muscari armeniacum", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum"}, {"subitem_title": "Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["453/454", "468/469/470"], "permalink_uri": "http://hdl.handle.net/10191/24666", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2014-01-08"}, "publish_date": "2014-01-08", "publish_status": "0", "recid": "2777", "relation": {}, "relation_version_is_last": true, "title": ["Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum"], "weko_shared_id": null}