@article{oai:niigata-u.repo.nii.ac.jp:00002777,
 author = {Suzuki, Sakae and Nakano, Masaru and Koike, Yosuke and Ueda, Kenji and Inoue, Masayasu and Nishihara, Masahiro and Yamamura, Saburo},
 issue = {1},
 journal = {園芸学会雑誌},
 month = {Jan},
 note = {Embryogenic calli of Muscari armeniacum cv. Blue Pearl were co-cultivated with Agrobac- terium tumefaciens EHA101/pBH, which harbored a binary vector that carries the phosphi- nothricin acetyltransferase (bar) and hygromycin phosphotransferase (hpf) genes. The calli were then transferred onto the selection media containing either 4 mg-liter^<-1> bialaphos or 75 mg- liter"1 hygromycin. Four to five weeks after transfer to the selection media, both bialaphos- resistant (Bia^r) and hygromycin-resistance (Hyg^r) cell clusters were produced. Over 90% of callus lines selected on a bialaphos-containing medium were verified to be transgenic by PCR analysis; this selection efficiency is comparable to that based on the hpt gene. Leaf segments of plantlets regenerated from the transgenic Bia^r callus lines showed resistance to 4 mg ・ liter^<-1> of bialaphos, indicating that the bar gene is useful not only as a selectable marker but also as a producer of herbicide-resistant transgenic plants of M. armeniacum., アグロバクテリウム法によるムスカリ(Muscari armericum cv. Blue pearl)の形質転換における選抜マーカー遺伝子barの有効性を調査した.葉片由来のエンブリオジエニックカルスをA.tumefaciens EHA101 / pBH(ビアラホス耐性遺伝子barおよびハイグロマイシン耐性遺伝子hptがT-DNA上にコードされている)との共存培養後,4mg・liter^<-1>ビアラホスまたは75 mg・liter^<-1>ハイグロマイシンを含む選抜培地に移植した.選抜開始4〜5週間後,ビアラホス耐性またはハイグロマイシン耐性カルスがそれぞれの選抜培地上で形成された. PCR分析の結果,陥r遺伝子を用いた場合の形質転換効率は90%以上となり,この効率は知z遺伝子を用いた場合の選抜効率と同等であった.また,ビアラホス耐性形質転換カルスから再生した植物体の葉片は4 mg・liter^<-1>ビアラホスに耐性を示した.これらの結果より,アグロバクテリウム法によるムスカリの形質転換において,bar遺伝子は有効な選抜マーカーとして利用できるだけでなく,除草剤耐性形質転換体の作出にも利用可能なことが明らかとなった.},
 pages = {60--62},
 title = {Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum},
 volume = {74},
 year = {2005}
}