{"_buckets": {"deposit": "2100ddea-f8f4-4f8f-aede-0c54aa2ced46"}, "_deposit": {"id": "25972", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25972"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00025972"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\uff23\u306e\u5358\u4e00\u30a6\u30b5\u30ae\u6d1e\u623f\u7d50\u7bc0\u7d30\u80de\uff2c\u578b\uff23\uff412 \u30c1\u30e3\u30cd\u30eb\u306b\u5bfe\u3059\u308b\u4f5c\u7528\uff1a\u30db\u30fc\u30eb\u30bb\u30eb\u3068\u30bb\u30eb\u30a2\u30bf\u30c3\u30c1\u30fb\u30d1\u30c3\u30c1\u30af\u30e9\u30f3\u30d7\u6cd5\u306b\u3088\u308b\u89e3\u6790"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1994-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "28", "bibliographicPageStart": "21", "bibliographicVolumeNumber": "24", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u6b6f\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u65b0\u6f5f\u6b6f\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Efects of phorbol esters (stimulator of protein kinase C) on the L-type Ca^2+ current in isolated single rabbit sino-atrial (SA) nodal cells were examined. In whole-cell voltage-clamp experiments, 12-0-tetradecanoylphorbo1-13-acetate (TPA) at 10^-7 M inhibited the L-type Ca^2+ current. Pretreatment with H-7 (10^-5 M), an antagonist of protein kinase C (PK-C), did not modify the TPA-induced depression. The time course of inactivation process for the L-type Ca^2 current was slowed. In cell-attached patch-clamp experiments, TPA (10^-7 M) profoundly decreased the probability of the opening of unitary L-type Ca^2+ channels (a dihydropyridine-sensitive). The conductance was unaffected (18 \u00b1 1pS, n=5). Even H-7(10^-5 M) alone tended to inhibit the opening of the channels. Addition of TPA (10^-7 M) to the H-7-containing solution caused further decrease in the opening. On the other hand, 4-alpha-phorbol-12, 13-didecanoate (not a PK-C activator) had no effect on the Ca^2+ channels. These results indicate that the PK-C Stimulation decreases the opening of L-type Ca^2+ channels of the SA nodal cell membranes.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "153319", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u4f50\u85e4, \u5e83\u5eb7"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u6b6f\u5b66\u4f1a"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN0018300X", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03850153", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Satoh, Hiroyasu"}], "nameIdentifiers": [{"nameIdentifier": "153318", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "NS_24(2)_21-28.pdf", "filesize": [{"value": "632.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 632900.0, "url": {"label": "NS_24(2)_21-28.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/25972/files/NS_24(2)_21-28.pdf"}, "version_id": "b877aaba-bfb1-41a8-95c5-9aa907e5763b"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Protein Kinase C", "subitem_subject_scheme": "Other"}, {"subitem_subject": "H-7", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Whole-cell clamp", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cell-attached patch clamp", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Single sino-atrial nodal cell", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Effects of Protein Kinase C on the L-Type Ca2+ Channel in Single Rabbit Sino-Atrial Nodal Cells : Analysis by Patch-Clamp Technique", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Effects of Protein Kinase C on the L-Type Ca2+ Channel in Single Rabbit Sino-Atrial Nodal Cells : Analysis by Patch-Clamp Technique"}, {"subitem_title": "Effects of Protein Kinase C on the L-Type Ca2+ Channel in Single Rabbit Sino-Atrial Nodal Cells : Analysis by Patch-Clamp Technique", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "432/1291/1292/1329"], "permalink_uri": "http://hdl.handle.net/10191/22904", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2013-08-07"}, "publish_date": "2013-08-07", "publish_status": "0", "recid": "25972", "relation": {}, "relation_version_is_last": true, "title": ["Effects of Protein Kinase C on the L-Type Ca2+ Channel in Single Rabbit Sino-Atrial Nodal Cells : Analysis by Patch-Clamp Technique"], "weko_shared_id": null}