{"created":"2021-03-01T06:31:30.221242+00:00","id":25972,"links":{},"metadata":{"_buckets":{"deposit":"2100ddea-f8f4-4f8f-aede-0c54aa2ced46"},"_deposit":{"id":"25972","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25972"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00025972","sets":["432:1291:1292:1329","453:456"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"プロテインキナーゼＣの単一ウサギ洞房結節細胞Ｌ型Ｃａ2 チャネルに対する作用：ホールセルとセルアタッチ・パッチクランプ法による解析"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"28","bibliographicPageStart":"21","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"新潟歯学会雑誌"},{"bibliographic_title":"新潟歯学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Efects of phorbol esters (stimulator of protein kinase C) on the L-type Ca^2+ current in isolated single rabbit sino-atrial (SA) nodal cells were examined. In whole-cell voltage-clamp experiments, 12-0-tetradecanoylphorbo1-13-acetate (TPA) at 10^-7 M inhibited the L-type Ca^2+ current. Pretreatment with H-7 (10^-5 M), an antagonist of protein kinase C (PK-C), did not modify the TPA-induced depression. The time course of inactivation process for the L-type Ca^2 current was slowed. In cell-attached patch-clamp experiments, TPA (10^-7 M) profoundly decreased the probability of the opening of unitary L-type Ca^2+ channels (a dihydropyridine-sensitive). The conductance was unaffected (18 ± 1pS, n=5). Even H-7(10^-5 M) alone tended to inhibit the opening of the channels. Addition of TPA (10^-7 M) to the H-7-containing solution caused further decrease in the opening. On the other hand, 4-alpha-phorbol-12, 13-didecanoate (not a PK-C activator) had no effect on the Ca^2+ channels. These results indicate that the PK-C Stimulation decreases the opening of L-type Ca^2+ channels of the SA nodal cell membranes.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"153319","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐藤, 広康"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟歯学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN0018300X","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03850153","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Satoh, Hiroyasu"}],"nameIdentifiers":[{"nameIdentifier":"153318","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-19"}],"displaytype":"detail","filename":"NS_24(2)_21-28.pdf","filesize":[{"value":"632.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"NS_24(2)_21-28.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/25972/files/NS_24(2)_21-28.pdf"},"version_id":"b877aaba-bfb1-41a8-95c5-9aa907e5763b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Protein Kinase C","subitem_subject_scheme":"Other"},{"subitem_subject":"H-7","subitem_subject_scheme":"Other"},{"subitem_subject":"Whole-cell clamp","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell-attached patch clamp","subitem_subject_scheme":"Other"},{"subitem_subject":"Single sino-atrial nodal cell","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of Protein Kinase C on the L-Type Ca2+ Channel in Single Rabbit Sino-Atrial Nodal Cells : Analysis by Patch-Clamp Technique","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of Protein Kinase C on the L-Type Ca2+ Channel in Single Rabbit Sino-Atrial Nodal Cells : Analysis by Patch-Clamp Technique"},{"subitem_title":"Effects of Protein Kinase C on the L-Type Ca2+ Channel in Single Rabbit Sino-Atrial Nodal Cells : Analysis by Patch-Clamp Technique","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1329"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-07"},"publish_date":"2013-08-07","publish_status":"0","recid":"25972","relation_version_is_last":true,"title":["Effects of Protein Kinase C on the L-Type Ca2+ Channel in Single Rabbit Sino-Atrial Nodal Cells : Analysis by Patch-Clamp Technique"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:55:59.270205+00:00"}