{"_buckets": {"deposit": "38e5edcc-bac1-4090-9a6d-730e5e93f83a"}, "_deposit": {"id": "2524", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2524"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002524"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "251", "bibliographicPageStart": "249", "bibliographicVolumeNumber": "22", "bibliographic_titles": [{"bibliographic_title": "Plant biotechnology"}, {"bibliographic_title": "Plant biotechnology", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Protoplasts were isolated from embryogenic calluses of Muscari armeniacum \u0027Blue Pearl\u0027, which had been subcultured for 3 years. Protoplasts started to divide after 5\u20137 days of culture, and colonies consisting of 50\u2013100 cells were produced after one month. The highest plating efficiency (10.9%) was obtained by using a medium containing 5.4 \u00b5M NAA and 4.4 \u00b5M BA, 0.5 M glucose and 2 g l\u22121 gellan gum. Protoplast-derived calluses produced somatic embryos at frequencies of 4.3\u201389.6% on media containing 0 or 0.54 \u00b5M NAA in combination with 0, 4.4, 22 or 44 \u00b5M BA, but few embryos converted into plantlets. On the other hand, over 35% of the calluses produced adventitious shoots on media containing 4.4 \u00b5M BA or 0.54 \u00b5M NAA in combination with 44 \u00b5M BA, and some of these shoots developed into plantlets following transfer to a medium without PGRs.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65e5\u672c\u690d\u7269\u7d30\u80de\u5206\u5b50\u751f\u7269\u5b66\u4f1a"}]}, "item_5_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11250821", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13424580", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nakano, Masaru"}], "nameIdentifiers": [{"nameIdentifier": "37610", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Shigefumi"}], "nameIdentifiers": [{"nameIdentifier": "37611", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kagami, Shiho"}], "nameIdentifiers": [{"nameIdentifier": "37612", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saito, Hiroyuki"}], "nameIdentifiers": [{"nameIdentifier": "37613", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "22_3_249-251.pdf", "filesize": [{"value": "330.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 330100.0, "url": {"label": "22_3_249-251.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/2524/files/22_3_249-251.pdf"}, "version_id": "853d0f5d-4f86-4c72-b9e2-3f9fb15c8ed8"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Adventitious shoot", "subitem_subject_scheme": "Other"}, {"subitem_subject": "muscari", "subitem_subject_scheme": "Other"}, {"subitem_subject": "plantlet regeneration", "subitem_subject_scheme": "Other"}, {"subitem_subject": "protoplast culture", "subitem_subject_scheme": "Other"}, {"subitem_subject": "somatic embryo", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl. ex Bak.", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl. ex Bak."}, {"subitem_title": "Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl. ex Bak.", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["453/454", "468/469/470"], "permalink_uri": "http://hdl.handle.net/10191/29867", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2014-09-10"}, "publish_date": "2014-09-10", "publish_status": "0", "recid": "2524", "relation": {}, "relation_version_is_last": true, "title": ["Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl. ex Bak."], "weko_shared_id": null}