@article{oai:niigata-u.repo.nii.ac.jp:00002524,
 author = {Nakano, Masaru and Tanaka, Shigefumi and Kagami, Shiho and Saito, Hiroyuki},
 issue = {3},
 journal = {Plant biotechnology},
 month = {Sep},
 note = {Protoplasts were isolated from embryogenic calluses of Muscari armeniacum 'Blue Pearl', which had been subcultured for 3 years. Protoplasts started to divide after 5–7 days of culture, and colonies consisting of 50–100 cells were produced after one month. The highest plating efficiency (10.9%) was obtained by using a medium containing 5.4 µM NAA and 4.4 µM BA, 0.5 M glucose and 2 g l−1 gellan gum. Protoplast-derived calluses produced somatic embryos at frequencies of 4.3–89.6% on media containing 0 or 0.54 µM NAA in combination with 0, 4.4, 22 or 44 µM BA, but few embryos converted into plantlets. On the other hand, over 35% of the calluses produced adventitious shoots on media containing 4.4 µM BA or 0.54 µM NAA in combination with 44 µM BA, and some of these shoots developed into plantlets following transfer to a medium without PGRs.},
 pages = {249--251},
 title = {Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl. ex Bak.},
 volume = {22},
 year = {2005}
}