{"_buckets": {"deposit": "9266f4de-95e3-4fd0-a9bd-36a2a3ff9f79"}, "_deposit": {"id": "2520", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2520"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002520"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "485", "bibliographicPageStart": "475", "bibliographicVolumeNumber": "23", "bibliographic_titles": [{"bibliographic_title": "Plant biotechnology"}, {"bibliographic_title": "Plant biotechnology", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The Golgi membranes labeled with cis-Golgi marker GFP-SYP31 were isolated from suspension-cultured cells of rice transformed with 35S::GFP-SYP31 by floating through a discontinuous sucrose density gradient in the presence of 5 mM MgCl2. The specific fluorescence intensity of final membrane preparation increased to approximately 150-fold in comparison with that of the post-nucleus soluble fraction. Specific activity of membrane-bound \u03b1-mannosidase (cis-Golgi) markedly increased, but NDPase (medial-/trans-Golgi) and NADPH-cytochrome c reductase endoplasmic reticulum were weakly detected in the membrane fraction. The other organelle marker enzymes, cytochrome c oxidase (mitochondria), alkaline pyrophosphatase (plastid), and catalase (peroxisome) were not detectable. Comparative display of protein spots in GFP-SYP31-labeled membranes, microsomal membranes and soluble fraction on the two dimensional gels showed that some proteins are markedably concentrated in the cis-Golgi membrane fraction. Furthermore, the mass spectrometric analysis of proteins separated by SDS-polyacrylamide gel electrophoresis indicated that the highly purified Golgi membranes contained several membrane traffic-related proteins and ER resident proteins. These findings indicated a close relationship between the ER and the cis-Golgi membranes.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65e5\u672c\u690d\u7269\u7d30\u80de\u5206\u5b50\u751f\u7269\u5b66\u4f1a"}]}, "item_5_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11250821", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13424580", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Asakura, Tsuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "37587", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirose, Shota"}], "nameIdentifiers": [{"nameIdentifier": "37588", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Katamine, Hiroki"}], "nameIdentifiers": [{"nameIdentifier": "37589", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitajima, Aya"}], "nameIdentifiers": [{"nameIdentifier": "37590", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "37591", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Masa"}], "nameIdentifiers": [{"nameIdentifier": "37592", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujiwara, Masayuki"}], "nameIdentifiers": [{"nameIdentifier": "37593", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimamoto, Ko"}], "nameIdentifiers": [{"nameIdentifier": "37594", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "37595", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ZL_23(5)_475-485.pdf", "filesize": [{"value": "2.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2400000.0, "url": {"label": "ZL_23(5)_475-485.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/2520/files/ZL_23(5)_475-485.pdf"}, "version_id": "e12cc0d8-8e02-4297-a648-67517c43dc32"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Cis-Golgi", "subitem_subject_scheme": "Other"}, {"subitem_subject": "green fluorescent protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "small GTPase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rice", "subitem_subject_scheme": "Other"}, {"subitem_subject": "syntaxins 31", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31"}, {"subitem_title": "Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["453/454", "485/486/487"], "permalink_uri": "http://hdl.handle.net/10191/17594", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2012-04-04"}, "publish_date": "2012-04-04", "publish_status": "0", "recid": "2520", "relation": {}, "relation_version_is_last": true, "title": ["Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31"], "weko_shared_id": null}