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  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 070 農学部
  2. 10 学術雑誌論文
  3. 10 査読済論文

Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31

http://hdl.handle.net/10191/17594
http://hdl.handle.net/10191/17594
5e8c20d6-a448-4efb-abb5-ed0d142c54bb
名前 / ファイル ライセンス アクション
ZL_23(5)_475-485.pdf ZL_23(5)_475-485.pdf (2.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-04-04
タイトル
言語 en
タイトル Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31
言語
言語 eng
キーワード
主題Scheme Other
主題 Cis-Golgi
キーワード
主題Scheme Other
主題 green fluorescent protein
キーワード
主題Scheme Other
主題 small GTPase
キーワード
主題Scheme Other
主題 rice
キーワード
主題Scheme Other
主題 syntaxins 31
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Asakura, Tsuyoshi

× Asakura, Tsuyoshi

WEKO 37587

en Asakura, Tsuyoshi

Search repository
Hirose, Shota

× Hirose, Shota

WEKO 37588

en Hirose, Shota

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Katamine, Hiroki

× Katamine, Hiroki

WEKO 37589

en Katamine, Hiroki

Search repository
Kitajima, Aya

× Kitajima, Aya

WEKO 37590

en Kitajima, Aya

Search repository
Hori, Hidetaka

× Hori, Hidetaka

WEKO 37591

en Hori, Hidetaka

Search repository
Sato, Masa

× Sato, Masa

WEKO 37592

en Sato, Masa

Search repository
Fujiwara, Masayuki

× Fujiwara, Masayuki

WEKO 37593

en Fujiwara, Masayuki

Search repository
Shimamoto, Ko

× Shimamoto, Ko

WEKO 37594

en Shimamoto, Ko

Search repository
Mitsui, Toshiaki

× Mitsui, Toshiaki

WEKO 37595

en Mitsui, Toshiaki

Search repository
抄録
内容記述タイプ Abstract
内容記述 The Golgi membranes labeled with cis-Golgi marker GFP-SYP31 were isolated from suspension-cultured cells of rice transformed with 35S::GFP-SYP31 by floating through a discontinuous sucrose density gradient in the presence of 5 mM MgCl2. The specific fluorescence intensity of final membrane preparation increased to approximately 150-fold in comparison with that of the post-nucleus soluble fraction. Specific activity of membrane-bound α-mannosidase (cis-Golgi) markedly increased, but NDPase (medial-/trans-Golgi) and NADPH-cytochrome c reductase endoplasmic reticulum were weakly detected in the membrane fraction. The other organelle marker enzymes, cytochrome c oxidase (mitochondria), alkaline pyrophosphatase (plastid), and catalase (peroxisome) were not detectable. Comparative display of protein spots in GFP-SYP31-labeled membranes, microsomal membranes and soluble fraction on the two dimensional gels showed that some proteins are markedably concentrated in the cis-Golgi membrane fraction. Furthermore, the mass spectrometric analysis of proteins separated by SDS-polyacrylamide gel electrophoresis indicated that the highly purified Golgi membranes contained several membrane traffic-related proteins and ER resident proteins. These findings indicated a close relationship between the ER and the cis-Golgi membranes.
書誌情報 en : Plant biotechnology

巻 23, 号 5, p. 475-485, 発行日 2006-12
出版者
言語 ja
出版者 日本植物細胞分子生物学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 1342-4580
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11250821
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.5511/plantbiotechnology.23.475
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
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