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Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31
http://hdl.handle.net/10191/17594
http://hdl.handle.net/10191/175945e8c20d6-a448-4efb-abb5-ed0d142c54bb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ZL_23(5)_475-485.pdf (2.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-04-04 | |||||
| タイトル | ||||||
| タイトル | Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cis-Golgi | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | green fluorescent protein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | small GTPase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | rice | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | syntaxins 31 | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Asakura, Tsuyoshi
× Asakura, Tsuyoshi× Hirose, Shota× Katamine, Hiroki× Kitajima, Aya× Hori, Hidetaka× Sato, Masa× Fujiwara, Masayuki× Shimamoto, Ko× Mitsui, Toshiaki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The Golgi membranes labeled with cis-Golgi marker GFP-SYP31 were isolated from suspension-cultured cells of rice transformed with 35S::GFP-SYP31 by floating through a discontinuous sucrose density gradient in the presence of 5 mM MgCl2. The specific fluorescence intensity of final membrane preparation increased to approximately 150-fold in comparison with that of the post-nucleus soluble fraction. Specific activity of membrane-bound α-mannosidase (cis-Golgi) markedly increased, but NDPase (medial-/trans-Golgi) and NADPH-cytochrome c reductase endoplasmic reticulum were weakly detected in the membrane fraction. The other organelle marker enzymes, cytochrome c oxidase (mitochondria), alkaline pyrophosphatase (plastid), and catalase (peroxisome) were not detectable. Comparative display of protein spots in GFP-SYP31-labeled membranes, microsomal membranes and soluble fraction on the two dimensional gels showed that some proteins are markedably concentrated in the cis-Golgi membrane fraction. Furthermore, the mass spectrometric analysis of proteins separated by SDS-polyacrylamide gel electrophoresis indicated that the highly purified Golgi membranes contained several membrane traffic-related proteins and ER resident proteins. These findings indicated a close relationship between the ER and the cis-Golgi membranes. | |||||
| 書誌情報 |
en : Plant biotechnology 巻 23, 号 5, p. 475-485, 発行日 2006-12 |
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| 出版者 | ||||||
| 出版者 | 日本植物細胞分子生物学会 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1342-4580 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11250821 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.5511/plantbiotechnology.23.475 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
