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Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31
http://hdl.handle.net/10191/17594
http://hdl.handle.net/10191/175945e8c20d6-a448-4efb-abb5-ed0d142c54bb
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-04-04 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cis-Golgi | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | green fluorescent protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | small GTPase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | rice | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | syntaxins 31 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Asakura, Tsuyoshi
× Asakura, Tsuyoshi× Hirose, Shota× Katamine, Hiroki× Kitajima, Aya× Hori, Hidetaka× Sato, Masa× Fujiwara, Masayuki× Shimamoto, Ko× Mitsui, Toshiaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The Golgi membranes labeled with cis-Golgi marker GFP-SYP31 were isolated from suspension-cultured cells of rice transformed with 35S::GFP-SYP31 by floating through a discontinuous sucrose density gradient in the presence of 5 mM MgCl2. The specific fluorescence intensity of final membrane preparation increased to approximately 150-fold in comparison with that of the post-nucleus soluble fraction. Specific activity of membrane-bound α-mannosidase (cis-Golgi) markedly increased, but NDPase (medial-/trans-Golgi) and NADPH-cytochrome c reductase endoplasmic reticulum were weakly detected in the membrane fraction. The other organelle marker enzymes, cytochrome c oxidase (mitochondria), alkaline pyrophosphatase (plastid), and catalase (peroxisome) were not detectable. Comparative display of protein spots in GFP-SYP31-labeled membranes, microsomal membranes and soluble fraction on the two dimensional gels showed that some proteins are markedably concentrated in the cis-Golgi membrane fraction. Furthermore, the mass spectrometric analysis of proteins separated by SDS-polyacrylamide gel electrophoresis indicated that the highly purified Golgi membranes contained several membrane traffic-related proteins and ER resident proteins. These findings indicated a close relationship between the ER and the cis-Golgi membranes. | |||||
書誌情報 |
en : Plant biotechnology 巻 23, 号 5, p. 475-485, 発行日 2006-12 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 日本植物細胞分子生物学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1342-4580 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11250821 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.5511/plantbiotechnology.23.475 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher |