{"_buckets": {"deposit": "259f4664-d7d2-415a-868a-2950d30e6a65"}, "_deposit": {"id": "2520", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2520"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002520", "sets": ["454", "487"]}, "author_link": ["37587", "37588", "37589", "37590", "37591", "37592", "37593", "37594", "37595"], "control_number": "2520", "item_1627363077551": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "485", "bibliographicPageStart": "475", "bibliographicVolumeNumber": "23", "bibliographic_titles": [{"bibliographic_title": "Plant biotechnology", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The Golgi membranes labeled with cis-Golgi marker GFP-SYP31 were isolated from suspension-cultured cells of rice transformed with 35S::GFP-SYP31 by floating through a discontinuous sucrose density gradient in the presence of 5 mM MgCl2. The specific fluorescence intensity of final membrane preparation increased to approximately 150-fold in comparison with that of the post-nucleus soluble fraction. Specific activity of membrane-bound α-mannosidase (cis-Golgi) markedly increased, but NDPase (medial-/trans-Golgi) and NADPH-cytochrome c reductase endoplasmic reticulum were weakly detected in the membrane fraction. The other organelle marker enzymes, cytochrome c oxidase (mitochondria), alkaline pyrophosphatase (plastid), and catalase (peroxisome) were not detectable. Comparative display of protein spots in GFP-SYP31-labeled membranes, microsomal membranes and soluble fraction on the two dimensional gels showed that some proteins are markedably concentrated in the cis-Golgi membrane fraction. Furthermore, the mass spectrometric analysis of proteins separated by SDS-polyacrylamide gel electrophoresis indicated that the highly purified Golgi membranes contained several membrane traffic-related proteins and ER resident proteins. These findings indicated a close relationship between the ER and the cis-Golgi membranes.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本植物細胞分子生物学会", "subitem_publisher_language": "ja"}]}, "item_5_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.5511/plantbiotechnology.23.475", "subitem_relation_type_select": "DOI"}}]}, "item_5_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11250821", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1342-4580", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Asakura, Tsuyoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37587", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirose, Shota", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37588", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Katamine, Hiroki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37589", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitajima, Aya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37590", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37591", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Masa", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37592", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujiwara, Masayuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37593", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimamoto, Ko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37594", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37595", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ZL_23(5)_475-485.pdf", "filesize": [{"value": "2.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2400000.0, "url": {"label": "ZL_23(5)_475-485.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/2520/files/ZL_23(5)_475-485.pdf"}, "version_id": "6d2fa7db-a619-4d1d-8dac-d8f6009aa2c6"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Cis-Golgi", "subitem_subject_scheme": "Other"}, {"subitem_subject": "green fluorescent protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "small GTPase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rice", "subitem_subject_scheme": "Other"}, {"subitem_subject": "syntaxins 31", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["454", "487"], "permalink_uri": "http://hdl.handle.net/10191/17594", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2012-04-04"}, "publish_date": "2012-04-04", "publish_status": "0", "recid": "2520", "relation": {}, "relation_version_is_last": true, "title": ["Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31"], "weko_shared_id": -1}