{"created":"2021-03-01T06:06:14.081636+00:00","id":2520,"links":{},"metadata":{"_buckets":{"deposit":"259f4664-d7d2-415a-868a-2950d30e6a65"},"_deposit":{"id":"2520","owners":[],"pid":{"revision_id":0,"type":"depid","value":"2520"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00002520","sets":["453:454","485:486:487"]},"author_link":["37587","37588","37589","37590","37591","37592","37593","37594","37595"],"control_number":"2520","item_1627363077551":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"485","bibliographicPageStart":"475","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"Plant biotechnology","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The Golgi membranes labeled with cis-Golgi marker GFP-SYP31 were isolated from suspension-cultured cells of rice transformed with 35S::GFP-SYP31 by floating through a discontinuous sucrose density gradient in the presence of 5 mM MgCl2. The specific fluorescence intensity of final membrane preparation increased to approximately 150-fold in comparison with that of the post-nucleus soluble fraction. Specific activity of membrane-bound α-mannosidase (cis-Golgi) markedly increased, but NDPase (medial-/trans-Golgi) and NADPH-cytochrome c reductase endoplasmic reticulum were weakly detected in the membrane fraction. The other organelle marker enzymes, cytochrome c oxidase (mitochondria), alkaline pyrophosphatase (plastid), and catalase (peroxisome) were not detectable. Comparative display of protein spots in GFP-SYP31-labeled membranes, microsomal membranes and soluble fraction on the two dimensional gels showed that some proteins are markedably concentrated in the cis-Golgi membrane fraction. Furthermore, the mass spectrometric analysis of proteins separated by SDS-polyacrylamide gel electrophoresis indicated that the highly purified Golgi membranes contained several membrane traffic-related proteins and ER resident proteins. These findings indicated a close relationship between the ER and the cis-Golgi membranes.","subitem_description_type":"Abstract"}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本植物細胞分子生物学会","subitem_publisher_language":"ja"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.5511/plantbiotechnology.23.475","subitem_relation_type_select":"DOI"}}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11250821","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1342-4580","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Asakura, Tsuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37587","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirose, Shota","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37588","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katamine, Hiroki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37589","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kitajima, Aya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37590","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hori, Hidetaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37591","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Masa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37592","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujiwara, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37593","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimamoto, Ko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37594","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitsui, Toshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37595","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"ZL_23(5)_475-485.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ZL_23(5)_475-485.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/2520/files/ZL_23(5)_475-485.pdf"},"version_id":"6d2fa7db-a619-4d1d-8dac-d8f6009aa2c6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cis-Golgi","subitem_subject_scheme":"Other"},{"subitem_subject":"green fluorescent protein","subitem_subject_scheme":"Other"},{"subitem_subject":"small GTPase","subitem_subject_scheme":"Other"},{"subitem_subject":"rice","subitem_subject_scheme":"Other"},{"subitem_subject":"syntaxins 31","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-04-04"},"publish_date":"2012-04-04","publish_status":"0","recid":"2520","relation_version_is_last":true,"title":["Isolation and proteomic analysis of rice Golgi membranes: cis-Golgi membranes labeled with GFP-SYP31"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:15:28.629203+00:00"}