{"_buckets": {"deposit": "b2261320-9fcc-495e-b447-6d02cf63ebbb"}, "_deposit": {"id": "23365", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "23365"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00023365"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Construction of Enzyme Activity of Diacylglycerol Kinase purified from Pig Thymus"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1988-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "509", "bibliographicPageStart": "501", "bibliographicVolumeNumber": "102", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "ATP: sn-1, 2-diacylglycerol phosphotransferase [EC 2.7.1.-., DG-kinase] was purified from pig thymus cytosol. The activity of the enzyme was reconstituted by the addition of either deoxycholate or phosphatidylcholine (PC). In addition, bovine serum albumin also activated DG-kinase activity. As was seen in the activation kinetics by PC alone, polymyxin B at 4\u03bcM in the presence of PC activated DG-kinase by increasing the number of activated enzyme. On the other hand, polymyxin B at 10\u03bcM inhibited DG-kinase by altering the interaction between 1,2-diacylglycerol and detergent. Similar inhibition was also observed by the addition of oleoyl CoA. Since oleoyl-CoA is a substrate of triacylglycerol synthesis, inhibitory effect of oleoyl-CoA on DG-kinase may play a regulatory role in vivo. Ca^\u003c2+\u003e at 10^\u003c-7\u003e M inhibited DG-kinase activity, suggesting a possible role of Ca^\u003c2+\u003e in modulation of DG-kinase activity in vivo. The inhibitory effect of p-mercuribenzoate on DG-kinase activity was partially relieved by the addition of dithiothreitol, indicating that the inhibition might be reversible. As compared with 1,3-diaclyglycerol, preincubation of DG-kinase with 1,2-diacylglycerol effectively protected p-mercuribenzoate inhibition. These observations suggest that DG-kinase may have a functional SH-group and can be regulated through hydrophobic interaction, oleoyl-CoA and Ca^\u003c2+\u003e in the process of biomembrane response systems.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "140790", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Iwata, Toyoto"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u533b\u5b66\u4f1a"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5ca9\u7530, \u8c4a\u4eba"}], "nameIdentifiers": [{"nameIdentifier": "140789", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "102(8)_501-509.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "102(8)_501-509.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/23365/files/102(8)_501-509.pdf"}, "version_id": "c1861720-34c9-46ea-947a-d3cbac5b4765"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "diacylglycerol kinase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "polymyxin B", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ca^\u003c2+\u003e", "subitem_subject_scheme": "Other"}, {"subitem_subject": "oleoyl-CoA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SH-reagent", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30b8\u30a2\u30b7\u30eb\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u30ad\u30ca\u30fc\u30bc", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30dd\u30ea\u30df\u30ad\u30b7\u30f3 B", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30ab\u30eb\u30b7\u30a6\u30e0", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30aa\u30ec\u30aa\u30a4\u30ebCoA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SH-\u8a66\u85ac", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "\u8c5a\u80f8\u817a\u3088\u308a\u7cbe\u88fd\u3057\u305fsn-1,2-\u30b8\u30a2\u30b7\u30eb\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u30ad\u30ca\u30fc\u30bc\u6d3b\u6027\u306e\u518d\u69cb\u6210", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "\u8c5a\u80f8\u817a\u3088\u308a\u7cbe\u88fd\u3057\u305fsn-1,2-\u30b8\u30a2\u30b7\u30eb\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u30ad\u30ca\u30fc\u30bc\u6d3b\u6027\u306e\u518d\u69cb\u6210"}, {"subitem_title": "\u8c5a\u80f8\u817a\u3088\u308a\u7cbe\u88fd\u3057\u305fsn-1,2-\u30b8\u30a2\u30b7\u30eb\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u30ad\u30ca\u30fc\u30bc\u6d3b\u6027\u306e\u518d\u69cb\u6210", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/538/1219"], "permalink_uri": "http://hdl.handle.net/10191/40899", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2016-04-06"}, "publish_date": "2016-04-06", "publish_status": "0", "recid": "23365", "relation": {}, "relation_version_is_last": true, "title": ["\u8c5a\u80f8\u817a\u3088\u308a\u7cbe\u88fd\u3057\u305fsn-1,2-\u30b8\u30a2\u30b7\u30eb\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u30ad\u30ca\u30fc\u30bc\u6d3b\u6027\u306e\u518d\u69cb\u6210"], "weko_shared_id": null}