WEKO3
AND
アイテム
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豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成
http://hdl.handle.net/10191/40899
f2b5a911-d38f-44e7-a87a-2a95417d362f
名前 / ファイル | ライセンス | アクション | |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-04-06 | |||||
タイトル | ||||||
タイトル | 豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | 豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | diacylglycerol kinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polymyxin B | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ca^<2+> | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | oleoyl-CoA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SH-reagent | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ジアシルグリセロールキナーゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ポリミキシン B | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | カルシウム | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | オレオイルCoA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SH-試薬 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Construction of Enzyme Activity of Diacylglycerol Kinase purified from Pig Thymus | |||||
著者 |
岩田, 豊人
× 岩田, 豊人 |
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著者別名 | ||||||
識別子 | ||||||
識別子 | 140790 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | Iwata, Toyoto | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | ATP: sn-1, 2-diacylglycerol phosphotransferase [EC 2.7.1.-., DG-kinase] was purified from pig thymus cytosol. The activity of the enzyme was reconstituted by the addition of either deoxycholate or phosphatidylcholine (PC). In addition, bovine serum albumin also activated DG-kinase activity. As was seen in the activation kinetics by PC alone, polymyxin B at 4μM in the presence of PC activated DG-kinase by increasing the number of activated enzyme. On the other hand, polymyxin B at 10μM inhibited DG-kinase by altering the interaction between 1,2-diacylglycerol and detergent. Similar inhibition was also observed by the addition of oleoyl CoA. Since oleoyl-CoA is a substrate of triacylglycerol synthesis, inhibitory effect of oleoyl-CoA on DG-kinase may play a regulatory role in vivo. Ca^<2+> at 10^<-7> M inhibited DG-kinase activity, suggesting a possible role of Ca^<2+> in modulation of DG-kinase activity in vivo. The inhibitory effect of p-mercuribenzoate on DG-kinase activity was partially relieved by the addition of dithiothreitol, indicating that the inhibition might be reversible. As compared with 1,3-diaclyglycerol, preincubation of DG-kinase with 1,2-diacylglycerol effectively protected p-mercuribenzoate inhibition. These observations suggest that DG-kinase may have a functional SH-group and can be regulated through hydrophobic interaction, oleoyl-CoA and Ca^<2+> in the process of biomembrane response systems. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 102, 号 8, p. 501-509, 発行日 1988-08 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |