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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 02 新潟医学会雑誌
  4. 第102巻第8号

豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成

http://hdl.handle.net/10191/40899
f2b5a911-d38f-44e7-a87a-2a95417d362f
名前 / ファイル ライセンス アクション
102(8)_501-509.pdf 102(8)_501-509.pdf (1.6 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2016-04-06
タイトル
タイトル 豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成
タイトル
言語 en
タイトル 豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成
言語
言語 jpn
キーワード
主題Scheme Other
主題 diacylglycerol kinase
キーワード
主題Scheme Other
主題 polymyxin B
キーワード
主題Scheme Other
主題 Ca^<2+>
キーワード
主題Scheme Other
主題 oleoyl-CoA
キーワード
主題Scheme Other
主題 SH-reagent
キーワード
主題Scheme Other
主題 ジアシルグリセロールキナーゼ
キーワード
主題Scheme Other
主題 ポリミキシン B
キーワード
主題Scheme Other
主題 カルシウム
キーワード
主題Scheme Other
主題 オレオイルCoA
キーワード
主題Scheme Other
主題 SH-試薬
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Construction of Enzyme Activity of Diacylglycerol Kinase purified from Pig Thymus
著者 岩田, 豊人

× 岩田, 豊人

WEKO 140789

岩田, 豊人

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著者別名
識別子
識別子 140790
識別子Scheme WEKO
姓名
姓名 Iwata, Toyoto
抄録
内容記述タイプ Abstract
内容記述 ATP: sn-1, 2-diacylglycerol phosphotransferase [EC 2.7.1.-., DG-kinase] was purified from pig thymus cytosol. The activity of the enzyme was reconstituted by the addition of either deoxycholate or phosphatidylcholine (PC). In addition, bovine serum albumin also activated DG-kinase activity. As was seen in the activation kinetics by PC alone, polymyxin B at 4μM in the presence of PC activated DG-kinase by increasing the number of activated enzyme. On the other hand, polymyxin B at 10μM inhibited DG-kinase by altering the interaction between 1,2-diacylglycerol and detergent. Similar inhibition was also observed by the addition of oleoyl CoA. Since oleoyl-CoA is a substrate of triacylglycerol synthesis, inhibitory effect of oleoyl-CoA on DG-kinase may play a regulatory role in vivo. Ca^<2+> at 10^<-7> M inhibited DG-kinase activity, suggesting a possible role of Ca^<2+> in modulation of DG-kinase activity in vivo. The inhibitory effect of p-mercuribenzoate on DG-kinase activity was partially relieved by the addition of dithiothreitol, indicating that the inhibition might be reversible. As compared with 1,3-diaclyglycerol, preincubation of DG-kinase with 1,2-diacylglycerol effectively protected p-mercuribenzoate inhibition. These observations suggest that DG-kinase may have a functional SH-group and can be regulated through hydrophobic interaction, oleoyl-CoA and Ca^<2+> in the process of biomembrane response systems.
書誌情報 新潟医学会雑誌
en : 新潟医学会雑誌

巻 102, 号 8, p. 501-509, 発行日 1988-08
出版者
出版者 新潟医学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 00290440
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00182415
著者版フラグ
値 publisher
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