{"_buckets": {"deposit": "b2261320-9fcc-495e-b447-6d02cf63ebbb"}, "_deposit": {"id": "23365", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "23365"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00023365", "sets": ["456", "1219"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Construction of Enzyme Activity of Diacylglycerol Kinase purified from Pig Thymus"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1988-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "509", "bibliographicPageStart": "501", "bibliographicVolumeNumber": "102", "bibliographic_titles": [{"bibliographic_title": "新潟医学会雑誌"}, {"bibliographic_title": "新潟医学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "ATP: sn-1, 2-diacylglycerol phosphotransferase [EC 2.7.1.-., DG-kinase] was purified from pig thymus cytosol. The activity of the enzyme was reconstituted by the addition of either deoxycholate or phosphatidylcholine (PC). In addition, bovine serum albumin also activated DG-kinase activity. As was seen in the activation kinetics by PC alone, polymyxin B at 4μM in the presence of PC activated DG-kinase by increasing the number of activated enzyme. On the other hand, polymyxin B at 10μM inhibited DG-kinase by altering the interaction between 1,2-diacylglycerol and detergent. Similar inhibition was also observed by the addition of oleoyl CoA. Since oleoyl-CoA is a substrate of triacylglycerol synthesis, inhibitory effect of oleoyl-CoA on DG-kinase may play a regulatory role in vivo. Ca^\u003c2+\u003e at 10^\u003c-7\u003e M inhibited DG-kinase activity, suggesting a possible role of Ca^\u003c2+\u003e in modulation of DG-kinase activity in vivo. The inhibitory effect of p-mercuribenzoate on DG-kinase activity was partially relieved by the addition of dithiothreitol, indicating that the inhibition might be reversible. As compared with 1,3-diaclyglycerol, preincubation of DG-kinase with 1,2-diacylglycerol effectively protected p-mercuribenzoate inhibition. These observations suggest that DG-kinase may have a functional SH-group and can be regulated through hydrophobic interaction, oleoyl-CoA and Ca^\u003c2+\u003e in the process of biomembrane response systems.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "140790", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Iwata, Toyoto"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟医学会"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "岩田, 豊人"}], "nameIdentifiers": [{"nameIdentifier": "140789", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "102(8)_501-509.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "102(8)_501-509.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/23365/files/102(8)_501-509.pdf"}, "version_id": "c1861720-34c9-46ea-947a-d3cbac5b4765"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "diacylglycerol kinase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "polymyxin B", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ca^\u003c2+\u003e", "subitem_subject_scheme": "Other"}, {"subitem_subject": "oleoyl-CoA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SH-reagent", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ジアシルグリセロールキナーゼ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ポリミキシン B", "subitem_subject_scheme": "Other"}, {"subitem_subject": "カルシウム", "subitem_subject_scheme": "Other"}, {"subitem_subject": "オレオイルCoA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SH-試薬", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成"}, {"subitem_title": "豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1219"], "permalink_uri": "http://hdl.handle.net/10191/40899", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-04-06"}, "publish_date": "2016-04-06", "publish_status": "0", "recid": "23365", "relation": {}, "relation_version_is_last": true, "title": ["豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成"], "weko_shared_id": null}