{"created":"2021-03-01T06:28:36.795564+00:00","id":23365,"links":{},"metadata":{"_buckets":{"deposit":"b2261320-9fcc-495e-b447-6d02cf63ebbb"},"_deposit":{"id":"23365","owners":[],"pid":{"revision_id":0,"type":"depid","value":"23365"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00023365","sets":["453:456","471:537:538:1219"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Construction of Enzyme Activity of Diacylglycerol Kinase purified from Pig Thymus"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1988-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"509","bibliographicPageStart":"501","bibliographicVolumeNumber":"102","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ATP: sn-1, 2-diacylglycerol phosphotransferase [EC 2.7.1.-., DG-kinase] was purified from pig thymus cytosol. The activity of the enzyme was reconstituted by the addition of either deoxycholate or phosphatidylcholine (PC). In addition, bovine serum albumin also activated DG-kinase activity. As was seen in the activation kinetics by PC alone, polymyxin B at 4μM in the presence of PC activated DG-kinase by increasing the number of activated enzyme. On the other hand, polymyxin B at 10μM inhibited DG-kinase by altering the interaction between 1,2-diacylglycerol and detergent. Similar inhibition was also observed by the addition of oleoyl CoA. Since oleoyl-CoA is a substrate of triacylglycerol synthesis, inhibitory effect of oleoyl-CoA on DG-kinase may play a regulatory role in vivo. Ca^<2+> at 10^<-7> M inhibited DG-kinase activity, suggesting a possible role of Ca^<2+> in modulation of DG-kinase activity in vivo. The inhibitory effect of p-mercuribenzoate on DG-kinase activity was partially relieved by the addition of dithiothreitol, indicating that the inhibition might be reversible. As compared with 1,3-diaclyglycerol, preincubation of DG-kinase with 1,2-diacylglycerol effectively protected p-mercuribenzoate inhibition. These observations suggest that DG-kinase may have a functional SH-group and can be regulated through hydrophobic interaction, oleoyl-CoA and Ca^<2+> in the process of biomembrane response systems.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"140790","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Iwata, Toyoto"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岩田, 豊人"}],"nameIdentifiers":[{"nameIdentifier":"140789","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-19"}],"displaytype":"detail","filename":"102(8)_501-509.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"102(8)_501-509.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/23365/files/102(8)_501-509.pdf"},"version_id":"c1861720-34c9-46ea-947a-d3cbac5b4765"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"diacylglycerol kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"polymyxin B","subitem_subject_scheme":"Other"},{"subitem_subject":"Ca^<2+>","subitem_subject_scheme":"Other"},{"subitem_subject":"oleoyl-CoA","subitem_subject_scheme":"Other"},{"subitem_subject":"SH-reagent","subitem_subject_scheme":"Other"},{"subitem_subject":"ジアシルグリセロールキナーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"ポリミキシン B","subitem_subject_scheme":"Other"},{"subitem_subject":"カルシウム","subitem_subject_scheme":"Other"},{"subitem_subject":"オレオイルCoA","subitem_subject_scheme":"Other"},{"subitem_subject":"SH-試薬","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成"},{"subitem_title":"豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1219"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-04-06"},"publish_date":"2016-04-06","publish_status":"0","recid":"23365","relation_version_is_last":true,"title":["豚胸腺より精製したsn-1,2-ジアシルグリセロールキナーゼ活性の再構成"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:53:37.456602+00:00"}