WEKO3
アイテム
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ヒト培養絨毛癌細胞のhCG産生分泌動態に及ぼすMTX, epidermal growth factor, LH-RHの影響
http://hdl.handle.net/10191/41302
http://hdl.handle.net/10191/413022fc07814-26f9-4154-88e4-0ce2b449a5aa
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-04-11 | |||||
タイトル | ||||||
タイトル | ヒト培養絨毛癌細胞のhCG産生分泌動態に及ぼすMTX, epidermal growth factor, LH-RHの影響 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | ヒト培養絨毛癌細胞のhCG産生分泌動態に及ぼすMTX, epidermal growth factor, LH-RHの影響 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MTX | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | epidermal growth factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | LH-RH | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hCG secretion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | choriocarcinoma cell line | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 上皮増殖因子 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hCG分泌 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 絨毛癌細胞株 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | The Effect of MTX, Epidermal Growth Factor, LH-RH on the Dynamics of hCG Synthesis and Secretion in Human Choriocarcinoma Cell Line | |||||
著者 |
須藤, 祐悦
× 須藤, 祐悦 |
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著者別名 | ||||||
識別子 | 138347 | |||||
識別子Scheme | WEKO | |||||
姓名 | Sudo, Yuetsu | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Mechanism of hCG synthesis and secretion in human trophoblastic cell is not clarified. In order to investigate the effect of Methotrexate (MTX), epidermal growth factor (EGF) and luteinizing hormone-releasing hormone (LH-RH), the established human choriocarcinoma cell line was used for evaluation of the secretion of native hCG and its subunits in culture media. Furthermore, The effect on cell growth, DNA synthesis ^3H-thymidine incorporation as well as hCG/LH and LH-RH receptor was analysed. The results were as follows. 1) Effect of MTX Significant increase of hCG-β secretion was observed but no decrease of cell growth at the concentration of 10^<-8>M. On the other hand, increase of native hCG and its subunits was observed at the concentration of 5×10^<-8>M with remarkable suppression of cell growth. These results are not consistent with the cell disintegration theory or DNA suppression theory for so called cellar effect. 2) Effect of EGF Significant Increase of cell growth was not observed at the concentration of 10, 10^2ng/ml. However, only hCG-β secretion was significantly increased at the concentration of 10^2ng/ml. 3) Effect of LH-RH It was at the concentration of 10, 10^2ng/ml that both cell growth and native hCG and its subunits secretion were significantly increased. However, at the concentration of 10^3ng/ml hCG subunits secretion were, on the contrary, decreased with increment of cell growth. Remarkable decrease was shown in corresponding maximum binding capacities (B max) for LH-RH receptor at the concentration of 10, 10^2, 10^3ng/ml. This is suggestive that down regulation mechanism is attributable to the chronic LH-RH administration. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 103, 号 6, p. 480-491, 発行日 1989-06 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |