@article{oai:niigata-u.repo.nii.ac.jp:00022878,
 author = {須藤, 祐悦},
 issue = {6},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Jun},
 note = {Mechanism of hCG synthesis and secretion in human trophoblastic cell is not clarified. In order to investigate the effect of Methotrexate (MTX), epidermal growth factor (EGF) and luteinizing hormone-releasing hormone (LH-RH), the established human choriocarcinoma cell line was used for evaluation of the secretion of native hCG and its subunits in culture media. Furthermore, The effect on cell growth, DNA synthesis ^3H-thymidine incorporation as well as hCG/LH and LH-RH receptor was analysed. The results were as follows. 1) Effect of MTX Significant increase of hCG-β secretion was observed but no decrease of cell growth at the concentration of 10^<-8>M. On the other hand, increase of native hCG and its subunits was observed at the concentration of 5×10^<-8>M with remarkable suppression of cell growth. These results are not consistent with the cell disintegration theory or DNA suppression theory for so called cellar effect. 2) Effect of EGF Significant Increase of cell growth was not observed at the concentration of 10, 10^2ng/ml. However, only hCG-β secretion was significantly increased at the concentration of 10^2ng/ml. 3) Effect of LH-RH It was at the concentration of 10, 10^2ng/ml that both cell growth and native hCG and its subunits secretion were significantly increased. However, at the concentration of 10^3ng/ml hCG subunits secretion were, on the contrary, decreased with increment of cell growth. Remarkable decrease was shown in corresponding maximum binding capacities (B max) for LH-RH receptor at the concentration of 10, 10^2, 10^3ng/ml. This is suggestive that down regulation mechanism is attributable to the chronic LH-RH administration.},
 pages = {480--491},
 title = {ヒト培養絨毛癌細胞のhCG産生分泌動態に及ぼすMTX, epidermal growth factor, LH-RHの影響},
 volume = {103},
 year = {1989}
}