{"_buckets": {"deposit": "a39a3461-7263-44a7-9b1e-7c57529e07cd"}, "_deposit": {"id": "2230", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2230"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002230", "sets": ["454", "487"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-08", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "3794", "bibliographicPageStart": "3788", "bibliographicVolumeNumber": "6", "bibliographic_titles": [{"bibliographic_title": "Organic \u0026 biomolecular chemistry"}, {"bibliographic_title": "Organic \u0026 biomolecular chemistry", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Lipid components from 12 nonpathogenic Mycobacterium species were analysed. A novel cyclic C(35)-terpene, named heptaprenylcycline , was obtained from 3 species, while octahydroheptaprenol , which has 3 Z-double bonds, was obtained from 6 species. The amounts of and in the cultured cells increased after the 4- to 6-d stationary phase. The yield of was considerably greater at a higher temperature of 37 degrees C than at an optimal temperature of 28 degrees C, while that of remained unchanged at all temperatures. A feeding experiment with d-[1-(13)C]glucose revealed that was produced via isopentenyl diphosphate, which is a metabolite of glycolysis and the methylerythritol phosphate pathway. The conversion of octahydroheptaprenyl diphosphate to was successful by using the cell-free extracts of M. chlorophenolicum, demonstrating that is the biosynthetic intermediate of . This is the first example of the biosynthesis of a natural terpene via the cyclisation of a linear C(35)-isoprenoid. The substrate for C(35)-terpene cyclase has Z-type prenyl moieties; however, terpene cyclases usually employ E-type isoprenoids. The gene encoding the terpene cyclase that cyclises prenyl diphosphate containing Z-double bonds as the natural substrate has not yet been detected. Despite a careful search using the FASTA3 program, we could not detect any gene that is homologous to the known diphosphate-triggered type of mono-, sesqui- and diterpene cyclases in the genome of M. vanbaalenii, the DNA sequence of which has recently been elucidated. This suggests that a novel type of terpene cyclase might exist in the nonpathogenic Mycobacterium species.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Royal Society of Chemistry"}]}, "item_5_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1039/b808513g", "subitem_relation_type_select": "DOI"}}]}, "item_5_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA1168650X", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "14770520", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sato, Tsutomu"}], "nameIdentifiers": [{"nameIdentifier": "7563", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kigawa, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "7564", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takagi, Ryosuke"}], "nameIdentifiers": [{"nameIdentifier": "7565", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Adachi, Tomomi"}], "nameIdentifiers": [{"nameIdentifier": "7566", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hoshino, Tsutomu"}], "nameIdentifiers": [{"nameIdentifier": "7567", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "6_3788-3794.pdf", "filesize": [{"value": "800.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 800000.0, "url": {"label": "6_3788-3794.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/2230/files/6_3788-3794.pdf"}, "version_id": "be9caf8d-afc6-4998-a99b-5ec8cbf3ab59"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Biosynthesis of a novel cyclic C35-terpene via the cyclisation of a Z-type C35-polyprenyl diphosphate obtained from a nonpathogenic Mycobacterium species", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Biosynthesis of a novel cyclic C35-terpene via the cyclisation of a Z-type C35-polyprenyl diphosphate obtained from a nonpathogenic Mycobacterium species"}, {"subitem_title": "Biosynthesis of a novel cyclic C35-terpene via the cyclisation of a Z-type C35-polyprenyl diphosphate obtained from a nonpathogenic Mycobacterium species", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["454", "487"], "permalink_uri": "http://hdl.handle.net/10191/25026", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-02-07"}, "publish_date": "2014-02-07", "publish_status": "0", "recid": "2230", "relation": {}, "relation_version_is_last": true, "title": ["Biosynthesis of a novel cyclic C35-terpene via the cyclisation of a Z-type C35-polyprenyl diphosphate obtained from a nonpathogenic Mycobacterium species"], "weko_shared_id": null}