{"created":"2021-03-01T06:05:55.730760+00:00","id":2230,"links":{},"metadata":{"_buckets":{"deposit":"a39a3461-7263-44a7-9b1e-7c57529e07cd"},"_deposit":{"id":"2230","owners":[],"pid":{"revision_id":0,"type":"depid","value":"2230"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00002230","sets":["453:454","485:486:487"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"3794","bibliographicPageStart":"3788","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Organic & biomolecular chemistry"},{"bibliographic_title":"Organic & biomolecular chemistry","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lipid components from 12 nonpathogenic Mycobacterium species were analysed. A novel cyclic C(35)-terpene, named heptaprenylcycline , was obtained from 3 species, while octahydroheptaprenol , which has 3 Z-double bonds, was obtained from 6 species. The amounts of and in the cultured cells increased after the 4- to 6-d stationary phase. The yield of was considerably greater at a higher temperature of 37 degrees C than at an optimal temperature of 28 degrees C, while that of remained unchanged at all temperatures. A feeding experiment with d-[1-(13)C]glucose revealed that was produced via isopentenyl diphosphate, which is a metabolite of glycolysis and the methylerythritol phosphate pathway. The conversion of octahydroheptaprenyl diphosphate to was successful by using the cell-free extracts of M. chlorophenolicum, demonstrating that is the biosynthetic intermediate of . This is the first example of the biosynthesis of a natural terpene via the cyclisation of a linear C(35)-isoprenoid. The substrate for C(35)-terpene cyclase has Z-type prenyl moieties; however, terpene cyclases usually employ E-type isoprenoids. The gene encoding the terpene cyclase that cyclises prenyl diphosphate containing Z-double bonds as the natural substrate has not yet been detected. Despite a careful search using the FASTA3 program, we could not detect any gene that is homologous to the known diphosphate-triggered type of mono-, sesqui- and diterpene cyclases in the genome of M. vanbaalenii, the DNA sequence of which has recently been elucidated. This suggests that a novel type of terpene cyclase might exist in the nonpathogenic Mycobacterium species.","subitem_description_type":"Abstract"}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Royal Society of Chemistry"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1039/b808513g","subitem_relation_type_select":"DOI"}}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1168650X","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14770520","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Tsutomu"}],"nameIdentifiers":[{"nameIdentifier":"7563","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kigawa, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"7564","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takagi, Ryosuke"}],"nameIdentifiers":[{"nameIdentifier":"7565","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Adachi, Tomomi"}],"nameIdentifiers":[{"nameIdentifier":"7566","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hoshino, Tsutomu"}],"nameIdentifiers":[{"nameIdentifier":"7567","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"6_3788-3794.pdf","filesize":[{"value":"800.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"6_3788-3794.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/2230/files/6_3788-3794.pdf"},"version_id":"be9caf8d-afc6-4998-a99b-5ec8cbf3ab59"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Biosynthesis of a novel cyclic C35-terpene via the cyclisation of a Z-type C35-polyprenyl diphosphate obtained from a nonpathogenic Mycobacterium species","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Biosynthesis of a novel cyclic C35-terpene via the cyclisation of a Z-type C35-polyprenyl diphosphate obtained from a nonpathogenic Mycobacterium species"},{"subitem_title":"Biosynthesis of a novel cyclic C35-terpene via the cyclisation of a Z-type C35-polyprenyl diphosphate obtained from a nonpathogenic Mycobacterium species","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-02-07"},"publish_date":"2014-02-07","publish_status":"0","recid":"2230","relation_version_is_last":true,"title":["Biosynthesis of a novel cyclic C35-terpene via the cyclisation of a Z-type C35-polyprenyl diphosphate obtained from a nonpathogenic Mycobacterium species"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:35:05.660181+00:00"}