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アイテム
モノクローナル抗体による膵癌細胞のCEA関連抗原の研究
http://hdl.handle.net/10191/34077
http://hdl.handle.net/10191/34077998e69c5-c854-43fc-ba66-0cd49c163098
名前 / ファイル | ライセンス | アクション |
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104(5)_348-356.pdf (2.5 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2015-11-09 | |||||
タイトル | ||||||
タイトル | モノクローナル抗体による膵癌細胞のCEA関連抗原の研究 | |||||
タイトル | ||||||
タイトル | モノクローナル抗体による膵癌細胞のCEA関連抗原の研究 | |||||
言語 | en | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | carcinoembryonic antigen | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nonspecific cross-reacting antigen | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | モノクローナル抗体 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 分子量 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 等電点 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Characterization of CEA Cross-reacting Antigen in a Pancreatic Cancer Cell Line using a Monoclonal Antibody | |||||
著者 |
内山, 一晃
× 内山, 一晃 |
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著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 135345 | |||||
姓名 | Uchiyama, Kazuaki | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Monoclonal antibodies (MAb) against pancreatic cancer were selected, and among them MAb 3H3, which reacted with CEA and CEA cross-reacting antigen, was used for this investigation. The Triton X-100 soluble nonspecific cross-reacting antigens (NCAs) recognized by MAb 3H3 have apparent mol. wts of 55,000 (NCA-55) in a pancreatic cancer cell line and 90,000 (NCA-90) in granulocytes using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). It was reported that, when granulocytes and epitherial cells in lung and pancreas were extracted with perchloric acid, mol. wts of 55,000 NCA existed in all of them, and mol. wet of 95,000 NCA existed only in granulocytes. But using Triton X-100 as detergent for the extraction, there have been almost no reports about this. In this paper, NCAs extracted by Triton X-100 were characterized by SDS-PAGE, western blotting, 2 dimensional electrophoresis using MAb 3H3. And the conclusions are summurized as follows: 1) When extracting NCAs using not perchloric acid but detergents, several NCAs exist in the extract. NCA-55 and NCA-90 are also extracted by the method. 2) NCA-55 exists in both perchloric acid and Triton X-100 treatment, so NCA-55 is not degradation product by perchloric acid treatment. 3) In a pancreatic cancer cell line (BxPC-3), NCA-55 is well produced with CEA, but NCA-90 is not produced. Although NCAs in granulocyte are consist of NCA-90 and NCA-55, NCA-55 exists less than NCA-90 in granulocyte. 4) NCA-55 from the pancreatic cancer cell line has chromatographically polymorphism. NCA-55 is consist of nearby four isoelectic points (pH 4.8, 5.3, 5.6, 5.8) 55 kD proteins, and pH 5.8 NCA-55 may more exist than others. But the four proteins have not so quantitative differences. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 104, 号 5, p. 348-356, 発行日 1990-05 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |