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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 02 新潟医学会雑誌
  4. 第104巻第5号

モノクローナル抗体による膵癌細胞のCEA関連抗原の研究

http://hdl.handle.net/10191/34077
http://hdl.handle.net/10191/34077
998e69c5-c854-43fc-ba66-0cd49c163098
名前 / ファイル ライセンス アクション
104(5)_348-356.pdf 104(5)_348-356.pdf (2.5 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-11-09
タイトル
タイトル モノクローナル抗体による膵癌細胞のCEA関連抗原の研究
タイトル
言語 en
タイトル モノクローナル抗体による膵癌細胞のCEA関連抗原の研究
言語
言語 jpn
キーワード
主題Scheme Other
主題 carcinoembryonic antigen
キーワード
主題Scheme Other
主題 nonspecific cross-reacting antigen
キーワード
主題Scheme Other
主題 モノクローナル抗体
キーワード
主題Scheme Other
主題 分子量
キーワード
主題Scheme Other
主題 等電点
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Characterization of CEA Cross-reacting Antigen in a Pancreatic Cancer Cell Line using a Monoclonal Antibody
著者 内山, 一晃

× 内山, 一晃

WEKO 135344

内山, 一晃

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著者別名
識別子 135345
識別子Scheme WEKO
姓名 Uchiyama, Kazuaki
抄録
内容記述タイプ Abstract
内容記述 Monoclonal antibodies (MAb) against pancreatic cancer were selected, and among them MAb 3H3, which reacted with CEA and CEA cross-reacting antigen, was used for this investigation. The Triton X-100 soluble nonspecific cross-reacting antigens (NCAs) recognized by MAb 3H3 have apparent mol. wts of 55,000 (NCA-55) in a pancreatic cancer cell line and 90,000 (NCA-90) in granulocytes using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). It was reported that, when granulocytes and epitherial cells in lung and pancreas were extracted with perchloric acid, mol. wts of 55,000 NCA existed in all of them, and mol. wet of 95,000 NCA existed only in granulocytes. But using Triton X-100 as detergent for the extraction, there have been almost no reports about this. In this paper, NCAs extracted by Triton X-100 were characterized by SDS-PAGE, western blotting, 2 dimensional electrophoresis using MAb 3H3. And the conclusions are summurized as follows: 1) When extracting NCAs using not perchloric acid but detergents, several NCAs exist in the extract. NCA-55 and NCA-90 are also extracted by the method. 2) NCA-55 exists in both perchloric acid and Triton X-100 treatment, so NCA-55 is not degradation product by perchloric acid treatment. 3) In a pancreatic cancer cell line (BxPC-3), NCA-55 is well produced with CEA, but NCA-90 is not produced. Although NCAs in granulocyte are consist of NCA-90 and NCA-55, NCA-55 exists less than NCA-90 in granulocyte. 4) NCA-55 from the pancreatic cancer cell line has chromatographically polymorphism. NCA-55 is consist of nearby four isoelectic points (pH 4.8, 5.3, 5.6, 5.8) 55 kD proteins, and pH 5.8 NCA-55 may more exist than others. But the four proteins have not so quantitative differences.
書誌情報 新潟医学会雑誌
en : 新潟医学会雑誌

巻 104, 号 5, p. 348-356, 発行日 1990-05
出版者
出版者 新潟医学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 00290440
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00182415
著者版フラグ
値 publisher
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