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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 02 新潟医学会雑誌
  4. 第106巻第10号

超急速凍結保存法を用いたマウス初期胚の生存性に関する検討

http://hdl.handle.net/10191/40061
http://hdl.handle.net/10191/40061
87cd7a2b-ec88-4e60-a580-db5c7ce1b31a
名前 / ファイル ライセンス アクション
106(10)_990-996.pdf 106(10)_990-996.pdf (1.4 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2016-03-25
タイトル
タイトル 超急速凍結保存法を用いたマウス初期胚の生存性に関する検討
タイトル
言語 en
タイトル 超急速凍結保存法を用いたマウス初期胚の生存性に関する検討
言語
言語 jpn
キーワード
主題Scheme Other
主題 Cryopreservation
キーワード
主題Scheme Other
主題 mouse preembryo
キーワード
主題Scheme Other
主題 ultrarapid freezing
キーワード
主題Scheme Other
主題 Dimethyl sulfoxide (DMSO)
キーワード
主題Scheme Other
主題 凍結保存
キーワード
主題Scheme Other
主題 マウス初期胚
キーワード
主題Scheme Other
主題 超急速凍結法
キーワード
主題Scheme Other
主題 ジメチルスルホキシド
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Effects on the Survival of mouse preembryos cryopreserved by Ultrarapid method of freezing
著者 七里, 和良

× 七里, 和良

WEKO 127721

七里, 和良

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著者別名
識別子 127722
識別子Scheme WEKO
姓名 Shichiri, Kazuyoshi
抄録
内容記述タイプ Abstract
内容記述 The usefulness of ultrarapid freezing of mouse preembryo was investigated in comparison with conventional rapid freezing. In the present experiments, 2-cell mouse embryos were frozen under some conditions in an attempt to optimize their survival and viability in vitro. The experiments showed that embryos exposed briefly (2 to 8 minutes) to relatively high concentrations of dimethyl sulfoxide (2 to 3.5 M) and 025 M sucrose survived and developed when they were plunged directly into liquid nitrogen and thawed in a 37℃ waterbath and removed by one step dilution. DMSO was observed to be a better cryoprotectant than Propandiol (PROH) for ultrarapid freezing. Mouse preembryos were cooled in a biological freezer using 1.5 M PROH as cryoprotectant. Thawing was done at room temperature and PROH was removed by multistep dilutions. The rate of survival and development to blastocyst of zygotes by rapid, programmed freezing using PROH was 85.7% and 45.5%, and that of 2-cell stages was 76.8% and 69.8%, respectively, Similar results were achieved by ultrarapid freezing using DMSO, i. e, the rate of survival and development to blastocyst was 89.0% and 59.2% concerning zygotes and 79.1% and 64.1% concerning 2-cell stages, respectively. In conclusion, ultrarapid freezing was considered to be as effective as conventional rapid freezing, as evaluated by their subsequent development in vitro.
書誌情報 新潟医学会雑誌
en : 新潟医学会雑誌

巻 106, 号 10, p. 990-996, 発行日 1992-10
出版者
出版者 新潟医学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 00290440
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00182415
著者版フラグ
値 publisher
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