@article{oai:niigata-u.repo.nii.ac.jp:00020735,
 author = {七里, 和良},
 issue = {10},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Oct},
 note = {The usefulness of ultrarapid freezing of mouse preembryo was investigated in comparison with conventional rapid freezing. In the present experiments, 2-cell mouse embryos were frozen under some conditions in an attempt to optimize their survival and viability in vitro. The experiments showed that embryos exposed briefly (2 to 8 minutes) to relatively high concentrations of dimethyl sulfoxide (2 to 3.5 M) and 025 M sucrose survived and developed when they were plunged directly into liquid nitrogen and thawed in a 37℃ waterbath and removed by one step dilution. DMSO was observed to be a better cryoprotectant than Propandiol (PROH) for ultrarapid freezing. Mouse preembryos were cooled in a biological freezer using 1.5 M PROH as cryoprotectant. Thawing was done at room temperature and PROH was removed by multistep dilutions. The rate of survival and development to blastocyst of zygotes by rapid, programmed freezing using PROH was 85.7% and 45.5%, and that of 2-cell stages was 76.8% and 69.8%, respectively, Similar results were achieved by ultrarapid freezing using DMSO, i. e, the rate of survival and development to blastocyst was 89.0% and 59.2% concerning zygotes and 79.1% and 64.1% concerning 2-cell stages, respectively. In conclusion, ultrarapid freezing was considered to be as effective as conventional rapid freezing, as evaluated by their subsequent development in vitro.},
 pages = {990--996},
 title = {超急速凍結保存法を用いたマウス初期胚の生存性に関する検討},
 volume = {106},
 year = {1992}
}