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Prevention of experimental autoimmune myocarditis by hydrodynamics-based naked Plasmid DNA encoding CTLA4-Ig gene delivery
http://hdl.handle.net/10191/1161
http://hdl.handle.net/10191/1161bea72513-eb75-4342-8415-578b861fe52f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
22_0001.pdf (608.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-04-23 | |||||
| タイトル | ||||||
| タイトル | Prevention of experimental autoimmune myocarditis by hydrodynamics-based naked Plasmid DNA encoding CTLA4-Ig gene delivery | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | gene therapy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | dilated cardiomyopathy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cytokine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | pCAGGS | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Abe, Satoru
× Abe, Satoru× Hanawa, Haruo× Hayashi, Manabu× Yoshida, Tsuyoshi× Komura, Satoru× Watanabe, Ritsuo× Lie, Hui× Chang, He× Kato, Kiminori× Kodama, Makoto× Maruyama, Hiroki× Nakazawa, Mikio× Miyazaki, Junichi× Aizawa, Yoshifusa |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 6609 | |||||
| 姓名 | 塙, 晴雄 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Rat experimental autoimmune myocarditis (EAM) was a T cell-mediated disease, which resembled the giant cell myocarditis seen in humans. Soluble CTLA4 improves some autoimmune diseases by blocking costimulatory signals on T cell. We investigated the effect of hydrodynamics-based naked plasmid DNA encoding CTLA4-immunoglobulin (Ig) gene delivery. Methods and Results: Lewis rats were immunized with cardiac myosin and treated with hydrodynamic-based transfection, namely a rapid tail vein injection of a large volume of pCAGGS encoding CTLA4-Ig chimera solution on Day 0. The vector-derived CTLA4-Ig mRNA expressions were mainly detected in the liver and plasma CTLA4-Ig protein levels were maintained at about 2 μg/ml during the experiment period. On Day 17, the ratio of heart to body weight, the amount of mRNA of atrial natriuretic peptide and the inflammatory areas in CTLA4 group were significantly lower than in the Control group treated with empty plasmid. Maximum rate of intraventricular pressure rise and decline (dp/dt), minimum dp/dt, LVEDP and central venous pressure improved significantly after treatment with CTLA4-Ig. On day 14, expressions of IL-2 in popliteal lymphnodes in the CTLA4-Ig group were significantly lower than in the Control group. Conclusions: Hydrodynamics-based transfection of plasmid encoding CTLA4-Ig chimera dramatically prevented EAM. | |||||
| 書誌情報 |
journal of cardiac failure en : journal of cardiac failure 巻 11, 号 7, p. 557-564, 発行日 2005-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 10719164 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11054062 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 16198253 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1016/j.cardfail.2005.04.005 | |||||
| 権利 | ||||||
| 権利情報 | Elsevier Inc. | |||||
| 著者版フラグ | ||||||
| 値 | author | |||||
