@article{oai:niigata-u.repo.nii.ac.jp:00002007,
 author = {Abe, Satoru and Hanawa, Haruo and Hayashi, Manabu and Yoshida, Tsuyoshi and Komura, Satoru and Watanabe, Ritsuo and Lie, Hui and Chang, He and Kato, Kiminori and Kodama, Makoto and Maruyama, Hiroki and Nakazawa, Mikio and Miyazaki, Junichi and Aizawa, Yoshifusa},
 issue = {7},
 journal = {journal of cardiac failure, journal of cardiac failure},
 month = {Sep},
 note = {Background: Rat experimental autoimmune myocarditis (EAM) was a T cell-mediated disease, which resembled the giant cell myocarditis seen in humans. Soluble CTLA4 improves some autoimmune diseases by blocking costimulatory signals on T cell. We investigated the effect of hydrodynamics-based naked plasmid DNA encoding CTLA4-immunoglobulin (Ig) gene delivery. Methods and Results: Lewis rats were immunized with cardiac myosin and treated with hydrodynamic-based transfection, namely a rapid tail vein injection of a large volume of pCAGGS encoding CTLA4-Ig chimera solution on Day 0. The vector-derived CTLA4-Ig mRNA expressions were mainly detected in the liver and plasma CTLA4-Ig protein levels were maintained at about 2 μg/ml during the experiment period. On Day 17, the ratio of heart to body weight, the amount of mRNA of atrial natriuretic peptide and the inflammatory areas in CTLA4 group were significantly lower than in the Control group treated with empty plasmid. Maximum rate of intraventricular pressure rise and decline (dp/dt), minimum dp/dt, LVEDP and central venous pressure improved significantly after treatment with CTLA4-Ig. On day 14, expressions of IL-2 in popliteal lymphnodes in the CTLA4-Ig group were significantly lower than in the Control group. Conclusions: Hydrodynamics-based transfection of plasmid encoding CTLA4-Ig chimera dramatically prevented EAM.},
 pages = {557--564},
 title = {Prevention of experimental autoimmune myocarditis by hydrodynamics-based naked Plasmid DNA encoding CTLA4-Ig gene delivery},
 volume = {11},
 year = {2005}
}