WEKO3
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2) 遺伝子マーキン : ヒト造血幹細への遺伝子導入(シンポジウム 遺伝子治療をめぐる諸問題, 第509回新潟医学会)
http://hdl.handle.net/10191/43251
http://hdl.handle.net/10191/4325108f9c4e6-7dc1-456d-8a10-e14b10602f9f
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-08-19 | |||||
タイトル | ||||||
タイトル | 2) 遺伝子マーキン : ヒト造血幹細への遺伝子導入(シンポジウム 遺伝子治療をめぐる諸問題, 第509回新潟医学会) | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | 2) 遺伝子マーキン : ヒト造血幹細への遺伝子導入(シンポジウム 遺伝子治療をめぐる諸問題, 第509回新潟医学会) | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | retrovirus vectors | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Neo-R gene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | spin transduction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hematopoietic stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | レトロウイルスベクター | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ネオマイシン耐性遺伝子 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 造血幹細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 導入効率 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Gene Marking : Retroviral Vector-Mediated Gene Transfer into CD34-Enriched Human Bone Marrow Stem Cells (Gene Therapy : Advances in Research and Treatment) | |||||
著者 |
森山, 美昭
× 森山, 美昭 |
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著者別名 | ||||||
識別子 | 112776 | |||||
識別子Scheme | WEKO | |||||
姓名 | Moriyama, Yoshiaki | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In order to improve the efficiency of gene transduction into human hematopoietic stem cells by retrovirus vectors, we conducted an in vitro study to determine the optimal conditions. Cells used as the target were CD34-enriched human bone marrow stem cells. Retrovirus vectors used were LNL6 and G1Na40, both carrying the Neomycin-resistant (Neo-R) gene as a genetic marker. Transduction efficiency varied from 5.1% to 35.0%, depending on the factors and procedures in the experiments, which cornprised MOI, the duration of exposure of cells to the vectors, and conduct/nonconduct of centrifuge of the cells during the exposure. The most effective gene transduction into CFV-GM was found to be a 48h-liquid culture with growth factors followed by 48h-vector exposure with the spin transduction. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 110, 号 8, p. 311-315, 発行日 1996-08 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |