{"created":"2021-03-01T06:22:40.995959+00:00","id":17963,"links":{},"metadata":{"_buckets":{"deposit":"a8edb898-7fbb-4cac-beb3-ed68a66e99c4"},"_deposit":{"id":"17963","owners":[],"pid":{"revision_id":0,"type":"depid","value":"17963"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00017963","sets":["453:456","471:537:538:1127"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Gene Marking : Retroviral Vector-Mediated Gene Transfer into CD34-Enriched Human Bone Marrow Stem Cells (Gene Therapy : Advances in Research and Treatment)"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"315","bibliographicPageStart":"311","bibliographicVolumeNumber":"110","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to improve the efficiency of gene transduction into human hematopoietic stem cells by retrovirus vectors, we conducted an in vitro study to determine the optimal conditions. Cells used as the target were CD34-enriched human bone marrow stem cells. Retrovirus vectors used were LNL6 and G1Na40, both carrying the Neomycin-resistant (Neo-R) gene as a genetic marker. Transduction efficiency varied from 5.1% to 35.0%, depending on the factors and procedures in the experiments, which cornprised MOI, the duration of exposure of cells to the vectors, and conduct/nonconduct of centrifuge of the cells during the exposure. The most effective gene transduction into CFV-GM was found to be a 48h-liquid culture with growth factors followed by 48h-vector exposure with the spin transduction.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"112776","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Moriyama, Yoshiaki"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森山, 美昭"}],"nameIdentifiers":[{"nameIdentifier":"112775","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-08"}],"displaytype":"detail","filename":"110(8)_311-315.pdf","filesize":[{"value":"632.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"110(8)_311-315.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/17963/files/110(8)_311-315.pdf"},"version_id":"8e527f08-3cd4-481b-9544-a4bccc60fd33"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"retrovirus vectors","subitem_subject_scheme":"Other"},{"subitem_subject":"Neo-R gene","subitem_subject_scheme":"Other"},{"subitem_subject":"spin transduction","subitem_subject_scheme":"Other"},{"subitem_subject":"hematopoietic stem cells","subitem_subject_scheme":"Other"},{"subitem_subject":"レトロウイルスベクター","subitem_subject_scheme":"Other"},{"subitem_subject":"ネオマイシン耐性遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"造血幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"導入効率","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"2) 遺伝子マーキン : ヒト造血幹細への遺伝子導入（シンポジウム 遺伝子治療をめぐる諸問題, 第509回新潟医学会)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"2) 遺伝子マーキン : ヒト造血幹細への遺伝子導入（シンポジウム 遺伝子治療をめぐる諸問題, 第509回新潟医学会)"},{"subitem_title":"2) 遺伝子マーキン : ヒト造血幹細への遺伝子導入（シンポジウム 遺伝子治療をめぐる諸問題, 第509回新潟医学会)","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1127"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-08-19"},"publish_date":"2016-08-19","publish_status":"0","recid":"17963","relation_version_is_last":true,"title":["2) 遺伝子マーキン : ヒト造血幹細への遺伝子導入（シンポジウム 遺伝子治療をめぐる諸問題, 第509回新潟医学会)"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:49:16.405579+00:00"}