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The samples were studied by measuring the total protein concentrations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The followings were observed;1)Total protein concentrations of the control group (control fraction) were 8.4±7.9 (mean±S.D.) mg/dl. 2)The concentrations of the 5-15 min fraction, 1 h fraction and 3 h fraction were 33.4±43.8mg/dl, 53.3±48.2mg/dl and 10.5±4.8mg/dl, respectively. 3)Statistical significance was;control fraction-5-15 min fraction (p\u003c0.01), control fraction-1 h fraction (p\u003c0.01), 5-15 min fraction-1 h fraction (p\u003c0.05), 1 h fraction-3 h fraction (p\u003c0.01), but no significance was seen between control fraction and 3 h fraction. 4)Urinary excretion protein amount at each fraction after running, and their total amount were also studied. 5-15 min fraction was 10.0±13.1mg, 1 h fraction was 26.7±24.1mg and 3 h fraction 5.2±2.4mg. The total amount of urinary protein excretion of 3 hours after 10 km running was 41.2±29.3mg. 5)Statistical study was done for the urinary protein excretion;5-15 min fraction-1 h fraction (p\u003c0.05), 1 h fraction-3 h fraction (p\u003c0.01), 5-15 min fraction-3 h fraction (no significance). 6)Peaks of total protein concentration were seen at 5-15 min fraction or 1 h fraction. Five protein bands (P1, P2, P3, P4 and P5) were seen by SDS-PAGE in almost all cases. P5 was the main band in the fractions except for the peak fractions. 7) By Western blotting, P1 was α2-macroglobulin, P2 was IgG, P4 was transferrin, P5 was albumin and P3 was the derivative of IgG. 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走運動による尿蛋白の電気泳動パターンの変化
http://hdl.handle.net/10191/44972
http://hdl.handle.net/10191/44972c45b0222-df1f-4b52-af2a-c2df5fc3b0d4
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-11-29 | |||||
タイトル | ||||||
タイトル | 走運動による尿蛋白の電気泳動パターンの変化 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | 走運動による尿蛋白の電気泳動パターンの変化 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | sports medicine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | physical exercise | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | urinalysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | urinary protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | electrophoresis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Western blotting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | スポーツ医学 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 運動負荷 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 尿分析 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 尿蛋白 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 電気泳動 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ウエスタン・ブロッティング | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Changes of Electrophoretic Patterns of Urinary Proteins after Running Exercise | |||||
著者 |
杉本, 英夫
× 杉本, 英夫 |
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著者別名 | ||||||
識別子 | 109498 | |||||
識別子Scheme | WEKO | |||||
姓名 | Sugimoto, Hideo | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | For the purpose of a better understanding of the changes of urinary proteins by exercise in healthy subjects, urine samples were collected from 30 healthy male students, 1 hour before (control fraciton), immediately (5-15 min fraction), 1 hour (1 h fraction) and 3 hours (3 h fraction) after 10km running at maximum speed. The samples were studied by measuring the total protein concentrations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The followings were observed;1)Total protein concentrations of the control group (control fraction) were 8.4±7.9 (mean±S.D.) mg/dl. 2)The concentrations of the 5-15 min fraction, 1 h fraction and 3 h fraction were 33.4±43.8mg/dl, 53.3±48.2mg/dl and 10.5±4.8mg/dl, respectively. 3)Statistical significance was;control fraction-5-15 min fraction (p<0.01), control fraction-1 h fraction (p<0.01), 5-15 min fraction-1 h fraction (p<0.05), 1 h fraction-3 h fraction (p<0.01), but no significance was seen between control fraction and 3 h fraction. 4)Urinary excretion protein amount at each fraction after running, and their total amount were also studied. 5-15 min fraction was 10.0±13.1mg, 1 h fraction was 26.7±24.1mg and 3 h fraction 5.2±2.4mg. The total amount of urinary protein excretion of 3 hours after 10 km running was 41.2±29.3mg. 5)Statistical study was done for the urinary protein excretion;5-15 min fraction-1 h fraction (p<0.05), 1 h fraction-3 h fraction (p<0.01), 5-15 min fraction-3 h fraction (no significance). 6)Peaks of total protein concentration were seen at 5-15 min fraction or 1 h fraction. Five protein bands (P1, P2, P3, P4 and P5) were seen by SDS-PAGE in almost all cases. P5 was the main band in the fractions except for the peak fractions. 7) By Western blotting, P1 was α2-macroglobulin, P2 was IgG, P4 was transferrin, P5 was albumin and P3 was the derivative of IgG. IgA was also detected in the position of P2. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 111, 号 6, p. 362-373, 発行日 1997-06 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |