{"created":"2021-03-01T06:22:00.477460+00:00","id":17341,"links":{},"metadata":{"_buckets":{"deposit":"3a773135-de54-4db9-8f98-cde249fb957a"},"_deposit":{"id":"17341","owners":[],"pid":{"revision_id":0,"type":"depid","value":"17341"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00017341","sets":["453:456","471:537:538:544"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Changes of Electrophoretic Patterns of Urinary Proteins after Running Exercise"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"373","bibliographicPageStart":"362","bibliographicVolumeNumber":"111","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"For the purpose of a better understanding of the changes of urinary proteins by exercise in healthy subjects, urine samples were collected from 30 healthy male students, 1 hour before (control fraciton), immediately (5－15 min fraction), 1 hour (1 h fraction) and 3 hours (3 h fraction) after 10km running at maximum speed. The samples were studied by measuring the total protein concentrations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The followings were observed；1)Total protein concentrations of the control group (control fraction) were 8.4±7.9 (mean±S.D.) mg/dl. 2)The concentrations of the 5－15 min fraction, 1 h fraction and 3 h fraction were 33.4±43.8mg/dl, 53.3±48.2mg/dl and 10.5±4.8mg/dl, respectively. 3)Statistical significance was；control fraction-5－15 min fraction (p<0.01), control fraction-1 h fraction (p<0.01), 5－15 min fraction-1 h fraction (p<0.05), 1 h fraction-3 h fraction (p<0.01), but no significance was seen between control fraction and 3 h fraction. 4)Urinary excretion protein amount at each fraction after running, and their total amount were also studied. 5－15 min fraction was 10.0±13.1mg, 1 h fraction was 26.7±24.1mg and 3 h fraction 5.2±2.4mg. The total amount of urinary protein excretion of 3 hours after 10 km running was 41.2±29.3mg. 5)Statistical study was done for the urinary protein excretion；5－15 min fraction-1 h fraction (p<0.05), 1 h fraction-3 h fraction (p<0.01), 5－15 min fraction-3 h fraction (no significance). 6)Peaks of total protein concentration were seen at 5－15 min fraction or 1 h fraction. Five protein bands (P1, P2, P3, P4 and P5) were seen by SDS-PAGE in almost all cases. P5 was the main band in the fractions except for the peak fractions. 7) By Western blotting, P1 was α2-macroglobulin, P2 was IgG, P4 was transferrin, P5 was albumin and P3 was the derivative of IgG. IgA was also detected in the position of P2.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"109498","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sugimoto, Hideo"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"杉本, 英夫"}],"nameIdentifiers":[{"nameIdentifier":"109497","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-08"}],"displaytype":"detail","filename":"111(6)_362-373.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"111(6)_362-373.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/17341/files/111(6)_362-373.pdf"},"version_id":"cb6351ce-aa04-4935-aa84-ac00381cc3c5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"sports medicine","subitem_subject_scheme":"Other"},{"subitem_subject":"physical exercise","subitem_subject_scheme":"Other"},{"subitem_subject":"urinalysis","subitem_subject_scheme":"Other"},{"subitem_subject":"urinary protein","subitem_subject_scheme":"Other"},{"subitem_subject":"electrophoresis","subitem_subject_scheme":"Other"},{"subitem_subject":"Western blotting","subitem_subject_scheme":"Other"},{"subitem_subject":"スポーツ医学","subitem_subject_scheme":"Other"},{"subitem_subject":"運動負荷","subitem_subject_scheme":"Other"},{"subitem_subject":"尿分析","subitem_subject_scheme":"Other"},{"subitem_subject":"尿蛋白","subitem_subject_scheme":"Other"},{"subitem_subject":"電気泳動","subitem_subject_scheme":"Other"},{"subitem_subject":"ウエスタン・ブロッティング","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"走運動による尿蛋白の電気泳動パターンの変化","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"走運動による尿蛋白の電気泳動パターンの変化"},{"subitem_title":"走運動による尿蛋白の電気泳動パターンの変化","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","544"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-11-29"},"publish_date":"2016-11-29","publish_status":"0","recid":"17341","relation_version_is_last":true,"title":["走運動による尿蛋白の電気泳動パターンの変化"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:48:42.446481+00:00"}