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マウス胎生期におけるカルビンジンD28k遺伝子の発現:レポーター遺伝子を用いたトランスジェニックマウスの解析
http://hdl.handle.net/10191/45733
http://hdl.handle.net/10191/45733b9685075-a7eb-4aee-afe3-9571f9163d02
名前 / ファイル | ライセンス | アクション |
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112(2)_67-75.pdf (1.3 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-01-20 | |||||
タイトル | ||||||
タイトル | マウス胎生期におけるカルビンジンD28k遺伝子の発現:レポーター遺伝子を用いたトランスジェニックマウスの解析 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | マウス胎生期におけるカルビンジンD28k遺伝子の発現:レポーター遺伝子を用いたトランスジェニックマウスの解析 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Spot35/Calbindin-D28k | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transgenic mouse β-galactosidase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Embryo | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Spot35蛋白/Calbindin-D28k | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | トランスジェニックマウスβ-ガラクトシダーゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 胎児 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | The Spot35/Calbindin-D28K Gene Expression in the Transgenic Mice | |||||
著者 |
中嶋, 治彦
× 中嶋, 治彦 |
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著者別名 | ||||||
識別子 | 106787 | |||||
識別子Scheme | WEKO | |||||
姓名 | Nakajima, Haruhiko | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Spot35/calbindin-D28K gene expression was examined in the early embryonic stages of transgenic mice. E.coli β-galactosidase was used as a reporter gene fused with the 3.9 kb promoter region in which transcription regulatory sequences and vitamin Dresponse element located. The eight amino acid residues at N-terminal ofβ-galactosidase were replaced with SV40 nuclear localization signal to distinguish from endogenousβ-galactosidase activity. The promoter drivedβ-galactosidase was discerned in nuclei of Purkinje cells in the cerebellum and distal renal tubes in the kidney. These expression pattern was compatible with the results obtained by immunocytochemistry with anti-Spot35 antibody. In this transgenic mouse the transgene expression was detected first on the tip of tail, limb buds and nose except for cerebellum, intestine and kidney where the protein is mainly present in adult. Immunohistochemical analyses against Spot35/calbindine antiserum showed coincident with the lacZ expression in these tissues. These findings might provide a new aspect concerning biological function of this protein in the early embryonic stages. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 112, 号 2, p. 67-75, 発行日 1998-02 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |