WEKO3
アイテム
{"_buckets": {"deposit": "2d336d70-3e20-46bc-8985-b7a1a7d92a71"}, "_deposit": {"id": "16442", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "16442"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00016442", "sets": ["456", "1104"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Effect of Epidermal Growth Factor on Migration Activity of Squamous Cell Carcinoma Cell."}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1998-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "9", "bibliographicPageEnd": "562", "bibliographicPageStart": "552", "bibliographicVolumeNumber": "112", "bibliographic_titles": [{"bibliographic_title": "新潟医学会雑誌"}, {"bibliographic_title": "新潟医学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "To investigate the effect of epidermal growth factor (EGF) on cell migration of squamous cell carcinoma (SCC) cells, migration, adhesion and spreading activity, organization of focal adhesion and actin stress fiber, integrin expression, and tyrosin phosphorylation of focal adhesion kinase (FAK) of a cell line were examined on type IV collagen in the presence of various concentrations of EGF. The cell line that was separated from an established cell line of SCC in uterine cervix, CaSki, expresses α2, α3, α6, β1 and β4 integrins, and was used in this study. Wound assay demonstrated that EGF increased the migration activity at 1 and 10 ng/ml, whereas it completely blocked the migration at 100 ng/ml. Blocking antibodies against α3, α6 and β1 integrins inhibited the migration in the absence of EGF, whereas antibodies against not only these integrins but also α2 inhibited it in the presence of 10 ng/ml EGF. Flowcytometry showed that EGF increased the expression of α2 and α6 integrins in a dose dependent manner. Attachment assay revealed that EGF decreased the adhesion activity to type IV collagen in a dose dependent manner. Morphological examinations showed that EGF inhibited the spreading at 10 and 100 ng/ml, and that the growth factor did not alter the organization of focal adhesions and actin stress fibers at 1 and 10 ng/ml, whereas it increased it at 100 ng/ml. Immunoprecipitation and immunoblot assays showed that EGF did not alter tyrosin phosphorylation level of FAK at any concentrations examined. These findings suggest that the EGF-mediated promotion of the migration at 1 and 10 ng/ml is related with the modulation of α2 integrin function and expression, and the inhibition of the adhesion, whereas EGF-mediated inhibition of the migration is related with the modulation of organization of actin stress fiber and focal adhesion, and that alteration in a tyrosin phosphorylation level of FAK is not always accompanied with an increase in the migration activity.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "104683", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Yamamoto, Yoko"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟医学会"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "山本, 洋子"}], "nameIdentifiers": [{"nameIdentifier": "104682", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-07"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "112(9)_552-562.pdf", "filesize": [{"value": "2.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2400000.0, "url": {"label": "112(9)_552-562.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/16442/files/112(9)_552-562.pdf"}, "version_id": "6042b320-d7c1-4742-bfe5-1060440c82cb"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "epidermal growth factor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "migration activity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "adhesion activity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "integrin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "focal adhesion kinase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Squamous cell carcinoma", "subitem_subject_scheme": "Other"}, {"subitem_subject": "上皮増殖因子", "subitem_subject_scheme": "Other"}, {"subitem_subject": "移動能", "subitem_subject_scheme": "Other"}, {"subitem_subject": "接着能", "subitem_subject_scheme": "Other"}, {"subitem_subject": "インテグリン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "有棘細胞癌", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "有棘細胞癌細胞の移動能に対するepidermal growth factorの影響", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "有棘細胞癌細胞の移動能に対するepidermal growth factorの影響"}, {"subitem_title": "有棘細胞癌細胞の移動能に対するepidermal growth factorの影響", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1104"], "permalink_uri": "http://hdl.handle.net/10191/46200", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-02-07"}, "publish_date": "2017-02-07", "publish_status": "0", "recid": "16442", "relation": {}, "relation_version_is_last": true, "title": ["有棘細胞癌細胞の移動能に対するepidermal growth factorの影響"], "weko_shared_id": null}
有棘細胞癌細胞の移動能に対するepidermal growth factorの影響
http://hdl.handle.net/10191/46200
http://hdl.handle.net/10191/46200a87cb2c5-3143-4dbc-aa77-f334654eaded
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-02-07 | |||||
タイトル | ||||||
タイトル | 有棘細胞癌細胞の移動能に対するepidermal growth factorの影響 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | 有棘細胞癌細胞の移動能に対するepidermal growth factorの影響 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | epidermal growth factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | migration activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | adhesion activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | integrin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | focal adhesion kinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Squamous cell carcinoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 上皮増殖因子 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 移動能 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 接着能 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | インテグリン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 有棘細胞癌 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Effect of Epidermal Growth Factor on Migration Activity of Squamous Cell Carcinoma Cell. | |||||
著者 |
山本, 洋子
× 山本, 洋子 |
|||||
著者別名 | ||||||
識別子 | 104683 | |||||
識別子Scheme | WEKO | |||||
姓名 | Yamamoto, Yoko | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To investigate the effect of epidermal growth factor (EGF) on cell migration of squamous cell carcinoma (SCC) cells, migration, adhesion and spreading activity, organization of focal adhesion and actin stress fiber, integrin expression, and tyrosin phosphorylation of focal adhesion kinase (FAK) of a cell line were examined on type IV collagen in the presence of various concentrations of EGF. The cell line that was separated from an established cell line of SCC in uterine cervix, CaSki, expresses α2, α3, α6, β1 and β4 integrins, and was used in this study. Wound assay demonstrated that EGF increased the migration activity at 1 and 10 ng/ml, whereas it completely blocked the migration at 100 ng/ml. Blocking antibodies against α3, α6 and β1 integrins inhibited the migration in the absence of EGF, whereas antibodies against not only these integrins but also α2 inhibited it in the presence of 10 ng/ml EGF. Flowcytometry showed that EGF increased the expression of α2 and α6 integrins in a dose dependent manner. Attachment assay revealed that EGF decreased the adhesion activity to type IV collagen in a dose dependent manner. Morphological examinations showed that EGF inhibited the spreading at 10 and 100 ng/ml, and that the growth factor did not alter the organization of focal adhesions and actin stress fibers at 1 and 10 ng/ml, whereas it increased it at 100 ng/ml. Immunoprecipitation and immunoblot assays showed that EGF did not alter tyrosin phosphorylation level of FAK at any concentrations examined. These findings suggest that the EGF-mediated promotion of the migration at 1 and 10 ng/ml is related with the modulation of α2 integrin function and expression, and the inhibition of the adhesion, whereas EGF-mediated inhibition of the migration is related with the modulation of organization of actin stress fiber and focal adhesion, and that alteration in a tyrosin phosphorylation level of FAK is not always accompanied with an increase in the migration activity. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 112, 号 9, p. 552-562, 発行日 1998-09 |
|||||
出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |