@article{oai:niigata-u.repo.nii.ac.jp:00016442,
 author = {山本, 洋子},
 issue = {9},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Sep},
 note = {To investigate the effect of epidermal growth factor (EGF) on cell migration of squamous cell carcinoma (SCC) cells, migration, adhesion and spreading activity, organization of focal adhesion and actin stress fiber, integrin expression, and tyrosin phosphorylation of focal adhesion kinase (FAK) of a cell line were examined on type IV collagen in the presence of various concentrations of EGF. The cell line that was separated from an established cell line of SCC in uterine cervix, CaSki, expresses α2, α3, α6, β1 and β4 integrins, and was used in this study. Wound assay demonstrated that EGF increased the migration activity at 1 and 10 ng/ml, whereas it completely blocked the migration at 100 ng/ml. Blocking antibodies against α3, α6 and β1 integrins inhibited the migration in the absence of EGF, whereas antibodies against not only these integrins but also α2 inhibited it in the presence of 10 ng/ml EGF. Flowcytometry showed that EGF increased the expression of α2 and α6 integrins in a dose dependent manner. Attachment assay revealed that EGF decreased the adhesion activity to type IV collagen in a dose dependent manner. Morphological examinations showed that EGF inhibited the spreading at 10 and 100 ng/ml, and that the growth factor did not alter the organization of focal adhesions and actin stress fibers at 1 and 10 ng/ml, whereas it increased it at 100 ng/ml. Immunoprecipitation and immunoblot assays showed that EGF did not alter tyrosin phosphorylation level of FAK at any concentrations examined. These findings suggest that the EGF-mediated promotion of the migration at 1 and 10 ng/ml is related with the modulation of α2 integrin function and expression, and the inhibition of the adhesion, whereas EGF-mediated inhibition of the migration is related with the modulation of organization of actin stress fiber and focal adhesion, and that alteration in a tyrosin phosphorylation level of FAK is not always accompanied with an increase in the migration activity.},
 pages = {552--562},
 title = {有棘細胞癌細胞の移動能に対するepidermal growth factorの影響},
 volume = {112},
 year = {1998}
}