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Functional analysis of the DXDDTA motif in squalene-hopene cyclase by site-directed mutagenesis experiments: initiation site of the polycyclization reaction and stabilization site of the carbocation intermediate of the initially cyclized A-ring.
http://hdl.handle.net/10191/6341
http://hdl.handle.net/10191/6341aafaa8f3-fb28-492e-b27d-c07277636893
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
07_0017.pdf (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-04-23 | |||||
| タイトル | ||||||
| タイトル | Functional analysis of the DXDDTA motif in squalene-hopene cyclase by site-directed mutagenesis experiments: initiation site of the polycyclization reaction and stabilization site of the carbocation intermediate of the initially cyclized A-ring. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | squalene | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | hopene | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | DXDDTA motif | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Alicyclobacillus acidocaldarius | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | oxidosqualene | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Sato, Tsutomu
× Sato, Tsutomu× Hoshino, Tsutomu |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 4377 | |||||
| 姓名 | 佐藤, 努 | |||||
| 言語 | en | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 4378 | |||||
| 姓名 | 星野, 力 | |||||
| 言語 | ja | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In order to clarify the function of the DXDDTA motif in squalene-hopene cyclase and to identify the acidic amino acid residues crucial for the catalysis, site-directed mutagenesis experiments were carried out. The following results were found: (1) residues D374 and D376 work for the initiation of polyolefin cyclization which arises from the proton attack on the terminal double bond; (2) residue D377 stabilizes C-10 carbocation of the initially cyclized A-ring intermediate, leading to subsequent B-ring closure, which was further verified by isolating the partially cyclized monocyclic product; (3) residues D313 and D447 outside the DXDDTA motif were identified as new active sites; (4) the H451 residue is likely to work in the protonated form to enhance the acidity of the carboxyl groups of D374 and/or D376. | |||||
| 書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 63, 号 12, p. 2189-2198, 発行日 1999 |
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| 出版者 | ||||||
| 出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0916-8451 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10824164 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1271/bbb.63.2189 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
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Cite as
Sato, Tsutomu, Hoshino, Tsutomu, 1999, Functional analysis of the DXDDTA motif in squalene-hopene cyclase by site-directed mutagenesis experiments: initiation site of the polycyclization reaction and stabilization site of the carbocation intermediate of the initially cyclized A-ring.: Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2189–2198 p.
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