{"created":"2021-03-01T06:05:14.680349+00:00","id":1573,"links":{},"metadata":{"_buckets":{"deposit":"2577c957-1ef6-40c4-93d4-07e614769921"},"_deposit":{"id":"1573","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1573"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00001573","sets":["453:454","485:486:487"]},"author_link":["4375","4376","4377","4378"],"control_number":"1573","item_1627363077551":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"2198","bibliographicPageStart":"2189","bibliographicVolumeNumber":"63","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to clarify the function of the DXDDTA motif in squalene-hopene cyclase and to identify the acidic amino acid residues crucial for the catalysis, site-directed mutagenesis experiments were carried out. The following results were found: (1) residues D374 and D376 work for the initiation of polyolefin cyclization which arises from the proton attack on the terminal double bond; (2) residue D377 stabilizes C-10 carbocation of the initially cyclized A-ring intermediate, leading to subsequent B-ring closure, which was further verified by isolating the partially cyclized monocyclic product; (3) residues D313 and D447 outside the DXDDTA motif were identified as new active sites; (4) the H451 residue is likely to work in the protonated form to enhance the acidity of the carboxyl groups of D374 and/or D376.","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"4377","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐藤, 努","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"4378","nameIdentifierScheme":"WEKO"}],"names":[{"name":"星野, 力","nameLang":"ja"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society for Bioscience, Biotechnology, and Agrochemistry","subitem_publisher_language":"en"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.1271/bbb.63.2189","subitem_relation_type_select":"DOI"}}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824164","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Tsutomu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4375","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hoshino, Tsutomu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4376","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"07_0017.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"07_0017.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/1573/files/07_0017.pdf"},"version_id":"43a74f2e-475c-4d8f-8a60-881e83a57718"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"squalene","subitem_subject_scheme":"Other"},{"subitem_subject":"hopene","subitem_subject_scheme":"Other"},{"subitem_subject":"DXDDTA motif","subitem_subject_scheme":"Other"},{"subitem_subject":"Alicyclobacillus acidocaldarius","subitem_subject_scheme":"Other"},{"subitem_subject":"oxidosqualene","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Functional analysis of the DXDDTA motif in squalene-hopene cyclase by site-directed mutagenesis experiments: initiation site of the polycyclization reaction and stabilization site of the carbocation intermediate of the initially cyclized A-ring.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Functional analysis of the DXDDTA motif in squalene-hopene cyclase by site-directed mutagenesis experiments: initiation site of the polycyclization reaction and stabilization site of the carbocation intermediate of the initially cyclized A-ring.","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-04-23"},"publish_date":"2008-04-23","publish_status":"0","recid":"1573","relation_version_is_last":true,"title":["Functional analysis of the DXDDTA motif in squalene-hopene cyclase by site-directed mutagenesis experiments: initiation site of the polycyclization reaction and stabilization site of the carbocation intermediate of the initially cyclized A-ring."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T03:34:20.239625+00:00"}