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The Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases from Wheat Bran of Triticum aestivum L. cv. Nourin #61
http://hdl.handle.net/10191/6334
http://hdl.handle.net/10191/6334826483fb-ebcb-4487-aa5a-11a443b5fc9e
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-04-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | The Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases from Wheat Bran of Triticum aestivum L. cv. Nourin #61 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Phytase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | inositol phosphates | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NMR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gas chromatography | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | dephosphorylation pathway | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Nakano, Tadao
× Nakano, Tadao× Joh, Toshio× Narita, Kazumasa× Hayakawa, Toshiro |
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著者別名 | ||||||
識別子 | 4367 | |||||
識別子Scheme | WEKO | |||||
姓名 | 城, 斗志夫 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1, 2, 3, 4, 5, 6-hexakisphosphate (InsP6). The pathway of hydrolysis of InsP6 by phytase from wheat bran of Triticum aestivum L. cv. Nourin #61 is proved in this study. Structures of the intermediates were established by a variety of nuclear magnetic resonance techniques (1H-, two-dimensional 1H-1H coupling-correlation spectra and two-dimensional 31P-1H correlation spectra), gas chromatography, and bioassay. On the basis of the structures identified, initial hydrolysis of the phosphate ester occurs at the D/L-4 position of InsP6 to yield D/L-Ins (1, 2, 3, 5, 6) P5. After the dephosphorylation, the pathway of dephosphorylation is divided into two routes. The main route proceeds via D/L-Ins (1, 2, 5, 6) P4, D/L-Ins (1, 2, 6) P3 and D/L-Ins (1, 2) P2, while the minor route proceeds via D/L-Ins (1, 2, 3, 6) P4, Ins (1, 2, 3) P3 and D/L-Ins (1, 2) P2. D/L-Ins (1, 2) P2 is hydrolyzed at the D/L-1 or 2-position, and finally myo-inositol is produced. | |||||
言語 | en | |||||
書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 64, 号 5, p. 995-1003, 発行日 2000 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-8451 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10824164 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1271/bbb.64.995 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher |