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The Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases from Wheat Bran of Triticum aestivum L. cv. Nourin #61
http://hdl.handle.net/10191/6334
http://hdl.handle.net/10191/6334826483fb-ebcb-4487-aa5a-11a443b5fc9e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
07_0010.pdf (994.7 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2008-04-23 | |||||
| タイトル | ||||||
| タイトル | The Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases from Wheat Bran of Triticum aestivum L. cv. Nourin #61 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Phytase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | inositol phosphates | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NMR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | gas chromatography | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | dephosphorylation pathway | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Nakano, Tadao
× Nakano, Tadao× Joh, Toshio× Narita, Kazumasa× Hayakawa, Toshiro |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 4367 | |||||
| 姓名 | 城, 斗志夫 | |||||
| 言語 | ja | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1, 2, 3, 4, 5, 6-hexakisphosphate (InsP6). The pathway of hydrolysis of InsP6 by phytase from wheat bran of Triticum aestivum L. cv. Nourin #61 is proved in this study. Structures of the intermediates were established by a variety of nuclear magnetic resonance techniques (1H-, two-dimensional 1H-1H coupling-correlation spectra and two-dimensional 31P-1H correlation spectra), gas chromatography, and bioassay. On the basis of the structures identified, initial hydrolysis of the phosphate ester occurs at the D/L-4 position of InsP6 to yield D/L-Ins (1, 2, 3, 5, 6) P5. After the dephosphorylation, the pathway of dephosphorylation is divided into two routes. The main route proceeds via D/L-Ins (1, 2, 5, 6) P4, D/L-Ins (1, 2, 6) P3 and D/L-Ins (1, 2) P2, while the minor route proceeds via D/L-Ins (1, 2, 3, 6) P4, Ins (1, 2, 3) P3 and D/L-Ins (1, 2) P2. D/L-Ins (1, 2) P2 is hydrolyzed at the D/L-1 or 2-position, and finally myo-inositol is produced. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 64, 号 5, p. 995-1003, 発行日 2000 |
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| 出版者 | ||||||
| 出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0916-8451 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10824164 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1271/bbb.64.995 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
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Cite as
Nakano, Tadao, Joh, Toshio, Narita, Kazumasa, Hayakawa, Toshiro, 2000, The Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases from Wheat Bran of Triticum aestivum L. cv. Nourin #61: Japan Society for Bioscience, Biotechnology, and Agrochemistry, 995–1003 p.
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