@article{oai:niigata-u.repo.nii.ac.jp:00001570,
 author = {Nakano, Tadao and Joh, Toshio and Narita, Kazumasa and Hayakawa, Toshiro},
 issue = {5},
 journal = {Bioscience, Biotechnology, and Biochemistry},
 month = {},
 note = {Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1, 2, 3, 4, 5, 6-hexakisphosphate (InsP6). The pathway of hydrolysis of InsP6 by phytase from wheat bran of Triticum aestivum L. cv. Nourin #61 is proved in this study. Structures of the intermediates were established by a variety of nuclear magnetic resonance techniques (1H-, two-dimensional 1H-1H coupling-correlation spectra and two-dimensional 31P-1H correlation spectra), gas chromatography, and bioassay. On the basis of the structures identified, initial hydrolysis of the phosphate ester occurs at the D/L-4 position of InsP6 to yield D/L-Ins (1, 2, 3, 5, 6) P5. After the dephosphorylation, the pathway of dephosphorylation is divided into two routes. The main route proceeds via D/L-Ins (1, 2, 5, 6) P4, D/L-Ins (1, 2, 6) P3 and D/L-Ins (1, 2) P2, while the minor route proceeds via D/L-Ins (1, 2, 3, 6) P4, Ins (1, 2, 3) P3 and D/L-Ins (1, 2) P2. D/L-Ins (1, 2) P2 is hydrolyzed at the D/L-1 or 2-position, and finally myo-inositol is produced.},
 pages = {995--1003},
 title = {The Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases from Wheat Bran of Triticum aestivum L. cv. Nourin #61},
 volume = {64},
 year = {2000}
}