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  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 070 農学部
  2. 10 学術雑誌論文
  3. 10 査読済論文

Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.

http://hdl.handle.net/10191/6337
http://hdl.handle.net/10191/6337
c8dce50e-a968-4048-878b-810ad5e383c8
名前 / ファイル ライセンス アクション
07_0013.pdf 07_0013.pdf (1.2 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-04-23
タイトル
タイトル Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.
言語 en
言語
言語 eng
キーワード
主題Scheme Other
主題 Chlorella vulgaris C-27
キーワード
主題Scheme Other
主題 cryoprotection
キーワード
主題Scheme Other
主題 freezing tolerance
キーワード
主題Scheme Other
主題 LEA protein
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Honjoh, Ken-ichi

× Honjoh, Ken-ichi

WEKO 4350

en Honjoh, Ken-ichi

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Matsumoto, Hiroko

× Matsumoto, Hiroko

WEKO 4351

en Matsumoto, Hiroko

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Shimizu, Hideyuki

× Shimizu, Hideyuki

WEKO 4352

en Shimizu, Hideyuki

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Ooyama, Kanae

× Ooyama, Kanae

WEKO 4353

en Ooyama, Kanae

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Tanaka, Kageyuki

× Tanaka, Kageyuki

WEKO 4354

en Tanaka, Kageyuki

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Oda, Yuichi

× Oda, Yuichi

WEKO 4355

en Oda, Yuichi

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Takata, Ryoji

× Takata, Ryoji

WEKO 4356

en Takata, Ryoji

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Joh, Toshio

× Joh, Toshio

WEKO 4357

en Joh, Toshio

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Suga, Koushirou

× Suga, Koushirou

WEKO 4358

en Suga, Koushirou

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Miyamoto, Takahisa

× Miyamoto, Takahisa

WEKO 4359

en Miyamoto, Takahisa

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Iio, Masayoshi

× Iio, Masayoshi

WEKO 4360

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Hatano, Shoji

× Hatano, Shoji

WEKO 4361

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著者別名
識別子Scheme WEKO
識別子 4362
姓名 城, 斗志夫
言語 ja
抄録
内容記述タイプ Abstract
内容記述 The nucleotide sequence of hiC12, isolated as a cDNA clone of hardening-induced Chlorella (hiC) genes, was identified. The clone encodes a late embryogenesis abundant (LEA) protein having six repeats of a 11-mer amino acid motif, although in a slightly imperfect form. To overexpress the hiC61) and hiC12 genes, their coding regions were PCR amplified and subcloned into a pGEX-1λT vector. The HIC6 and HIC12 proteins were expressed as GST fusion proteins in E. coli, then purified. The two HIC proteins were found to be effective in protecting a freeze-labile enzyme, LDH, against freeze-inactivation. On a molar concentration basis, they were about 3.1×106 times more effective in protecting LDH than sucrose and as effective as BSA. Cryoprotection tests with five kinds of chain-shortened polypeptides, synthesized based on the 11-mer amino acid motif of the HIC6 protein showed that the cryoprotective activity decreased with a decrease in the repeating units of the 11-mer motif. In fact, cryoprotective activities of three kinds of single 11-mer amino acids were very low even at high concentrations. All the results suggested that the sufficiently repeated 11-mer motif is required for the cryoprotective activities of Chlorella LEA proteins.
言語 en
書誌情報 en : Bioscience, Biotechnology, and Biochemistry

巻 64, 号 8, p. 1656-1663, 発行日 2000
出版者
出版者 Japan Society for Bioscience, Biotechnology, and Agrochemistry
言語 en
ISSN
収録物識別子タイプ ISSN
収録物識別子 0916-8451
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10824164
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.1271/bbb.64.1656
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
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