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Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.
http://hdl.handle.net/10191/6337
http://hdl.handle.net/10191/6337c8dce50e-a968-4048-878b-810ad5e383c8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
07_0013.pdf (1.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2008-04-23 | |||||
| タイトル | ||||||
| タイトル | Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chlorella vulgaris C-27 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cryoprotection | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | freezing tolerance | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | LEA protein | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Honjoh, Ken-ichi
× Honjoh, Ken-ichi× Matsumoto, Hiroko× Shimizu, Hideyuki× Ooyama, Kanae× Tanaka, Kageyuki× Oda, Yuichi× Takata, Ryoji× Joh, Toshio× Suga, Koushirou× Miyamoto, Takahisa× Iio, Masayoshi× Hatano, Shoji |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 4362 | |||||
| 姓名 | 城, 斗志夫 | |||||
| 言語 | ja | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The nucleotide sequence of hiC12, isolated as a cDNA clone of hardening-induced Chlorella (hiC) genes, was identified. The clone encodes a late embryogenesis abundant (LEA) protein having six repeats of a 11-mer amino acid motif, although in a slightly imperfect form. To overexpress the hiC61) and hiC12 genes, their coding regions were PCR amplified and subcloned into a pGEX-1λT vector. The HIC6 and HIC12 proteins were expressed as GST fusion proteins in E. coli, then purified. The two HIC proteins were found to be effective in protecting a freeze-labile enzyme, LDH, against freeze-inactivation. On a molar concentration basis, they were about 3.1×106 times more effective in protecting LDH than sucrose and as effective as BSA. Cryoprotection tests with five kinds of chain-shortened polypeptides, synthesized based on the 11-mer amino acid motif of the HIC6 protein showed that the cryoprotective activity decreased with a decrease in the repeating units of the 11-mer motif. In fact, cryoprotective activities of three kinds of single 11-mer amino acids were very low even at high concentrations. All the results suggested that the sufficiently repeated 11-mer motif is required for the cryoprotective activities of Chlorella LEA proteins. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 64, 号 8, p. 1656-1663, 発行日 2000 |
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| 出版者 | ||||||
| 出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0916-8451 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10824164 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1271/bbb.64.1656 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
