ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "24bf0ef1-3287-4279-bbef-c6892ea59fbf"}, "_deposit": {"id": "1569", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1569"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00001569", "sets": ["454", "487"]}, "author_link": ["4350", "4351", "4352", "4353", "4354", "4355", "4356", "4357", "4358", "4359", "4360", "4361", "4362"], "control_number": "1569", "item_1627363077551": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2000", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "1663", "bibliographicPageStart": "1656", "bibliographicVolumeNumber": "64", "bibliographic_titles": [{"bibliographic_title": "Bioscience, Biotechnology, and Biochemistry", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The nucleotide sequence of hiC12, isolated as a cDNA clone of hardening-induced Chlorella (hiC) genes, was identified. The clone encodes a late embryogenesis abundant (LEA) protein having six repeats of a 11-mer amino acid motif, although in a slightly imperfect form. To overexpress the hiC61) and hiC12 genes, their coding regions were PCR amplified and subcloned into a pGEX-1λT vector. The HIC6 and HIC12 proteins were expressed as GST fusion proteins in E. coli, then purified. The two HIC proteins were found to be effective in protecting a freeze-labile enzyme, LDH, against freeze-inactivation. On a molar concentration basis, they were about 3.1×106 times more effective in protecting LDH than sucrose and as effective as BSA. Cryoprotection tests with five kinds of chain-shortened polypeptides, synthesized based on the 11-mer amino acid motif of the HIC6 protein showed that the cryoprotective activity decreased with a decrease in the repeating units of the 11-mer motif. In fact, cryoprotective activities of three kinds of single 11-mer amino acids were very low even at high concentrations. All the results suggested that the sufficiently repeated 11-mer motif is required for the cryoprotective activities of Chlorella LEA proteins.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_5_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "4362", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "城, 斗志夫", "nameLang": "ja"}]}]}, "item_5_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Japan Society for Bioscience, Biotechnology, and Agrochemistry", "subitem_publisher_language": "en"}]}, "item_5_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.1271/bbb.64.1656", "subitem_relation_type_select": "DOI"}}]}, "item_5_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10824164", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0916-8451", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Honjoh, Ken-ichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4350", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Hiroko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4351", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimizu, Hideyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4352", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ooyama, Kanae", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4353", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Kageyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4354", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oda, Yuichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4355", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takata, Ryoji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4356", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Joh, Toshio", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4357", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suga, Koushirou", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4358", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miyamoto, Takahisa", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4359", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iio, Masayoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4360", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hatano, Shoji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4361", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "07_0013.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1200000.0, "url": {"label": "07_0013.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/1569/files/07_0013.pdf"}, "version_id": "87856974-a6b7-4771-ade2-85c190efc43b"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Chlorella vulgaris C-27", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cryoprotection", "subitem_subject_scheme": "Other"}, {"subitem_subject": "freezing tolerance", "subitem_subject_scheme": "Other"}, {"subitem_subject": "LEA protein", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["454", "487"], "permalink_uri": "http://hdl.handle.net/10191/6337", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-04-23"}, "publish_date": "2008-04-23", "publish_status": "0", "recid": "1569", "relation": {}, "relation_version_is_last": true, "title": ["Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27."], "weko_shared_id": -1}
  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 070 農学部
  2. 10 学術雑誌論文
  3. 10 査読済論文

Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.

http://hdl.handle.net/10191/6337
http://hdl.handle.net/10191/6337
c8dce50e-a968-4048-878b-810ad5e383c8
名前 / ファイル ライセンス アクション
07_0013.pdf 07_0013.pdf (1.2 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-04-23
タイトル
言語 en
タイトル Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.
言語
言語 eng
キーワード
主題Scheme Other
主題 Chlorella vulgaris C-27
キーワード
主題Scheme Other
主題 cryoprotection
キーワード
主題Scheme Other
主題 freezing tolerance
キーワード
主題Scheme Other
主題 LEA protein
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Honjoh, Ken-ichi

× Honjoh, Ken-ichi

WEKO 4350

en Honjoh, Ken-ichi

Search repository
Matsumoto, Hiroko

× Matsumoto, Hiroko

WEKO 4351

en Matsumoto, Hiroko

Search repository
Shimizu, Hideyuki

× Shimizu, Hideyuki

WEKO 4352

en Shimizu, Hideyuki

Search repository
Ooyama, Kanae

× Ooyama, Kanae

WEKO 4353

en Ooyama, Kanae

Search repository
Tanaka, Kageyuki

× Tanaka, Kageyuki

WEKO 4354

en Tanaka, Kageyuki

Search repository
Oda, Yuichi

× Oda, Yuichi

WEKO 4355

en Oda, Yuichi

Search repository
Takata, Ryoji

× Takata, Ryoji

WEKO 4356

en Takata, Ryoji

Search repository
Joh, Toshio

× Joh, Toshio

WEKO 4357

en Joh, Toshio

Search repository
Suga, Koushirou

× Suga, Koushirou

WEKO 4358

en Suga, Koushirou

Search repository
Miyamoto, Takahisa

× Miyamoto, Takahisa

WEKO 4359

en Miyamoto, Takahisa

Search repository
Iio, Masayoshi

× Iio, Masayoshi

WEKO 4360

en Iio, Masayoshi

Search repository
Hatano, Shoji

× Hatano, Shoji

WEKO 4361

en Hatano, Shoji

Search repository
著者別名
識別子 4362
識別子Scheme WEKO
姓名 城, 斗志夫
言語 ja
抄録
内容記述タイプ Abstract
内容記述 The nucleotide sequence of hiC12, isolated as a cDNA clone of hardening-induced Chlorella (hiC) genes, was identified. The clone encodes a late embryogenesis abundant (LEA) protein having six repeats of a 11-mer amino acid motif, although in a slightly imperfect form. To overexpress the hiC61) and hiC12 genes, their coding regions were PCR amplified and subcloned into a pGEX-1λT vector. The HIC6 and HIC12 proteins were expressed as GST fusion proteins in E. coli, then purified. The two HIC proteins were found to be effective in protecting a freeze-labile enzyme, LDH, against freeze-inactivation. On a molar concentration basis, they were about 3.1×106 times more effective in protecting LDH than sucrose and as effective as BSA. Cryoprotection tests with five kinds of chain-shortened polypeptides, synthesized based on the 11-mer amino acid motif of the HIC6 protein showed that the cryoprotective activity decreased with a decrease in the repeating units of the 11-mer motif. In fact, cryoprotective activities of three kinds of single 11-mer amino acids were very low even at high concentrations. All the results suggested that the sufficiently repeated 11-mer motif is required for the cryoprotective activities of Chlorella LEA proteins.
言語 en
書誌情報 en : Bioscience, Biotechnology, and Biochemistry

巻 64, 号 8, p. 1656-1663, 発行日 2000
出版者
言語 en
出版者 Japan Society for Bioscience, Biotechnology, and Agrochemistry
ISSN
収録物識別子タイプ ISSN
収録物識別子 0916-8451
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10824164
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.1271/bbb.64.1656
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 20:57:52.554239
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3