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Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.
http://hdl.handle.net/10191/6337
http://hdl.handle.net/10191/6337c8dce50e-a968-4048-878b-810ad5e383c8
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-04-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chlorella vulgaris C-27 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cryoprotection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | freezing tolerance | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | LEA protein | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Honjoh, Ken-ichi
× Honjoh, Ken-ichi× Matsumoto, Hiroko× Shimizu, Hideyuki× Ooyama, Kanae× Tanaka, Kageyuki× Oda, Yuichi× Takata, Ryoji× Joh, Toshio× Suga, Koushirou× Miyamoto, Takahisa× Iio, Masayoshi× Hatano, Shoji |
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著者別名 | ||||||
識別子 | 4362 | |||||
識別子Scheme | WEKO | |||||
姓名 | 城, 斗志夫 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The nucleotide sequence of hiC12, isolated as a cDNA clone of hardening-induced Chlorella (hiC) genes, was identified. The clone encodes a late embryogenesis abundant (LEA) protein having six repeats of a 11-mer amino acid motif, although in a slightly imperfect form. To overexpress the hiC61) and hiC12 genes, their coding regions were PCR amplified and subcloned into a pGEX-1λT vector. The HIC6 and HIC12 proteins were expressed as GST fusion proteins in E. coli, then purified. The two HIC proteins were found to be effective in protecting a freeze-labile enzyme, LDH, against freeze-inactivation. On a molar concentration basis, they were about 3.1×106 times more effective in protecting LDH than sucrose and as effective as BSA. Cryoprotection tests with five kinds of chain-shortened polypeptides, synthesized based on the 11-mer amino acid motif of the HIC6 protein showed that the cryoprotective activity decreased with a decrease in the repeating units of the 11-mer motif. In fact, cryoprotective activities of three kinds of single 11-mer amino acids were very low even at high concentrations. All the results suggested that the sufficiently repeated 11-mer motif is required for the cryoprotective activities of Chlorella LEA proteins. | |||||
言語 | en | |||||
書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 64, 号 8, p. 1656-1663, 発行日 2000 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-8451 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10824164 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1271/bbb.64.1656 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher |