{"_buckets": {"deposit": "16bab213-dd0f-4fb6-8182-529ee7597f8b"}, "_deposit": {"id": "1569", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1569"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00001569"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2000", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "1663", "bibliographicPageStart": "1656", "bibliographicVolumeNumber": "64", "bibliographic_titles": [{"bibliographic_title": "Bioscience, Biotechnology, and Biochemistry"}, {"bibliographic_title": "Bioscience, Biotechnology, and Biochemistry", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The nucleotide sequence of hiC12, isolated as a cDNA clone of hardening-induced Chlorella (hiC) genes, was identified. The clone encodes a late embryogenesis abundant (LEA) protein having six repeats of a 11-mer amino acid motif, although in a slightly imperfect form. To overexpress the hiC61) and hiC12 genes, their coding regions were PCR amplified and subcloned into a pGEX-1\u03bbT vector. The HIC6 and HIC12 proteins were expressed as GST fusion proteins in E. coli, then purified. The two HIC proteins were found to be effective in protecting a freeze-labile enzyme, LDH, against freeze-inactivation. On a molar concentration basis, they were about 3.1\u00d7106 times more effective in protecting LDH than sucrose and as effective as BSA. Cryoprotection tests with five kinds of chain-shortened polypeptides, synthesized based on the 11-mer amino acid motif of the HIC6 protein showed that the cryoprotective activity decreased with a decrease in the repeating units of the 11-mer motif. In fact, cryoprotective activities of three kinds of single 11-mer amino acids were very low even at high concentrations. All the results suggested that the sufficiently repeated 11-mer motif is required for the cryoprotective activities of Chlorella LEA proteins.", "subitem_description_type": "Abstract"}]}, "item_5_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "4362", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u57ce, \u6597\u5fd7\u592b"}]}]}, "item_5_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Japan Society for Bioscience, Biotechnology, and Agrochemistry"}]}, "item_5_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10824164", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "09168451", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Honjoh, Ken-ichi"}], "nameIdentifiers": [{"nameIdentifier": "4350", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Hiroko"}], "nameIdentifiers": [{"nameIdentifier": "4351", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimizu, Hideyuki"}], "nameIdentifiers": [{"nameIdentifier": "4352", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ooyama, Kanae"}], "nameIdentifiers": [{"nameIdentifier": "4353", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Kageyuki"}], "nameIdentifiers": [{"nameIdentifier": "4354", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oda, Yuichi"}], "nameIdentifiers": [{"nameIdentifier": "4355", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takata, Ryoji"}], "nameIdentifiers": [{"nameIdentifier": "4356", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Joh, Toshio"}], "nameIdentifiers": [{"nameIdentifier": "4357", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suga, Koushirou"}], "nameIdentifiers": [{"nameIdentifier": "4358", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miyamoto, Takahisa"}], "nameIdentifiers": [{"nameIdentifier": "4359", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iio, Masayoshi"}], "nameIdentifiers": [{"nameIdentifier": "4360", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hatano, Shoji"}], "nameIdentifiers": [{"nameIdentifier": "4361", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "07_0013.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1200000.0, "url": {"label": "07_0013.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/1569/files/07_0013.pdf"}, "version_id": "d04e1c41-1c19-4c9f-92b4-0977131dcb2e"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Chlorella vulgaris C-27", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cryoprotection", "subitem_subject_scheme": "Other"}, {"subitem_subject": "freezing tolerance", "subitem_subject_scheme": "Other"}, {"subitem_subject": "LEA protein", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27."}, {"subitem_title": "Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["453/454", "485/486/487"], "permalink_uri": "http://hdl.handle.net/10191/6337", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2008-04-23"}, "publish_date": "2008-04-23", "publish_status": "0", "recid": "1569", "relation": {}, "relation_version_is_last": true, "title": ["Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27."], "weko_shared_id": null}