{"created":"2021-03-01T06:05:14.439948+00:00","id":1569,"links":{},"metadata":{"_buckets":{"deposit":"24bf0ef1-3287-4279-bbef-c6892ea59fbf"},"_deposit":{"id":"1569","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1569"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00001569","sets":["453:454","485:486:487"]},"author_link":["4350","4351","4352","4353","4354","4355","4356","4357","4358","4359","4360","4361","4362"],"control_number":"1569","item_1627363077551":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"1663","bibliographicPageStart":"1656","bibliographicVolumeNumber":"64","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The nucleotide sequence of hiC12, isolated as a cDNA clone of hardening-induced Chlorella (hiC) genes, was identified. The clone encodes a late embryogenesis abundant (LEA) protein having six repeats of a 11-mer amino acid motif, although in a slightly imperfect form. To overexpress the hiC61) and hiC12 genes, their coding regions were PCR amplified and subcloned into a pGEX-1λT vector. The HIC6 and HIC12 proteins were expressed as GST fusion proteins in E. coli, then purified. The two HIC proteins were found to be effective in protecting a freeze-labile enzyme, LDH, against freeze-inactivation. On a molar concentration basis, they were about 3.1×106 times more effective in protecting LDH than sucrose and as effective as BSA. Cryoprotection tests with five kinds of chain-shortened polypeptides, synthesized based on the 11-mer amino acid motif of the HIC6 protein showed that the cryoprotective activity decreased with a decrease in the repeating units of the 11-mer motif. In fact, cryoprotective activities of three kinds of single 11-mer amino acids were very low even at high concentrations. All the results suggested that the sufficiently repeated 11-mer motif is required for the cryoprotective activities of Chlorella LEA proteins.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"4362","nameIdentifierScheme":"WEKO"}],"names":[{"name":"城, 斗志夫","nameLang":"ja"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society for Bioscience, Biotechnology, and Agrochemistry","subitem_publisher_language":"en"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.1271/bbb.64.1656","subitem_relation_type_select":"DOI"}}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824164","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Honjoh, Ken-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4350","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Hiroko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4351","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Hideyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4352","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ooyama, Kanae","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4353","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Kageyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4354","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oda, Yuichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4355","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takata, Ryoji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4356","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Joh, Toshio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4357","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suga, Koushirou","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4358","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyamoto, Takahisa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4359","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iio, Masayoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4360","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hatano, Shoji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4361","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"07_0013.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"07_0013.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/1569/files/07_0013.pdf"},"version_id":"87856974-a6b7-4771-ade2-85c190efc43b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chlorella vulgaris C-27","subitem_subject_scheme":"Other"},{"subitem_subject":"cryoprotection","subitem_subject_scheme":"Other"},{"subitem_subject":"freezing tolerance","subitem_subject_scheme":"Other"},{"subitem_subject":"LEA protein","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27.","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-04-23"},"publish_date":"2008-04-23","publish_status":"0","recid":"1569","relation_version_is_last":true,"title":["Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C-27."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T03:34:20.205563+00:00"}