WEKO3
アイテム
An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars
http://hdl.handle.net/10191/1804
http://hdl.handle.net/10191/1804d1838e09-65e7-400d-965b-7b83eba50a04
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2007-05-10 | |||||
タイトル | ||||||
タイトル | An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell Proliferation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell Differentiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Heat-Shock Proteins | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Immunohistochemistry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Molar | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Odontogenesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rats | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakasone, Naohiro
× Nakasone, Naohiro× Yoshie, Hiromasa× Ohshima, Hayato |
|||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 14 | |||||
姓名 | 大島, 勇人 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Objectives: The aim of this study is to clarify the functional significance of heat-shock protein (HSP)-25 during tooth development. Design: We compared the expression of HSP-25 in the dental epithelial and mesenchymal cells with their proliferative activity during odontogenesis in rat molars on postnatal Days 1–100 by immunohistochemistry using anti-HSP-25 and anti-5-bromo-2’-deoxyuridine (BrdU) for cell proliferation assay. Results: On Day one, BrdU-immunoreactive cells were densely located in the inner enamel epithelium in the cervical loop and inter-cusped areas and the dental pulp adjacent to them, whereas HSP-25-immunoractivity (IR) was restricted to the cusped area where odontoblasts and ameloblasts had already differentiated. Subsequently, BrdU-IR shifted in the apical direction to be localized around Hertwig’s epithelial root sheath during Days 5–30, never overlapping with concomitantly apically-shifted HSP-25-IR. On Days 60–100, BrdU-immunoreactive cells were hardly recognizable in the dental pulp, where HSP-25-IR was exclusively localized in the odontoblast layer. Furthermore, the odontoblast- and ameloblast-lineage cells exhibited two steps in the expression of HSP-25 throughout the postnatal stages: first, dental epithelial and pulpal mesenchymal cells showed a weak IR for HSP-25 after the cessation of their proliferative activity, and subsequently odontoblasts and ameloblasts consistently expressed an intense HSP-25-IR. Conclusion: Odontoblast- and ameloblast-lineage cells acquire HSP-25-IR after they complete their cell division, suggesting that this protein acts as a switch between cell proliferation and differentiation during tooth development. The consistent expression of HSP-25-IR in the formative cells may be involved in the maintenance of their functional integrity. | |||||
書誌情報 |
Archives of Oral Biology en : Archives of Oral Biology 巻 51, 号 5, p. 378-386, 発行日 2006-05 |
|||||
出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00039969 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00548457 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.archoralbio.2005.09.007 | |||||
権利 | ||||||
権利情報 | Elsevier Ltd | |||||
著者版フラグ | ||||||
値 | author |