{"_buckets": {"deposit": "57d3e950-aa94-4367-9f8a-13f36d0dc88f"}, "_deposit": {"id": "1549", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1549"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00001549"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "386", "bibliographicPageStart": "378", "bibliographicVolumeNumber": "51", "bibliographic_titles": [{"bibliographic_title": "Archives of Oral Biology"}, {"bibliographic_title": "Archives of Oral Biology", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Objectives: The aim of this study is to clarify the functional significance of heat-shock protein (HSP)-25 during tooth development. Design: We compared the expression of HSP-25 in the dental epithelial and mesenchymal cells with their proliferative activity during odontogenesis in rat molars on postnatal Days 1\u2013100 by immunohistochemistry using anti-HSP-25 and anti-5-bromo-2\u2019-deoxyuridine (BrdU) for cell proliferation assay. Results: On Day one, BrdU-immunoreactive cells were densely located in the inner enamel epithelium in the cervical loop and inter-cusped areas and the dental pulp adjacent to them, whereas HSP-25-immunoractivity (IR) was restricted to the cusped area where odontoblasts and ameloblasts had already differentiated. Subsequently, BrdU-IR shifted in the apical direction to be localized around Hertwig\u2019s epithelial root sheath during Days 5\u201330, never overlapping with concomitantly apically-shifted HSP-25-IR. On Days 60\u2013100, BrdU-immunoreactive cells were hardly recognizable in the dental pulp, where HSP-25-IR was exclusively localized in the odontoblast layer. Furthermore, the odontoblast- and ameloblast-lineage cells exhibited two steps in the expression of HSP-25 throughout the postnatal stages: first, dental epithelial and pulpal mesenchymal cells showed a weak IR for HSP-25 after the cessation of their proliferative activity, and subsequently odontoblasts and ameloblasts consistently expressed an intense HSP-25-IR. Conclusion: Odontoblast- and ameloblast-lineage cells acquire HSP-25-IR after they complete their cell division, suggesting that this protein acts as a switch between cell proliferation and differentiation during tooth development. The consistent expression of HSP-25-IR in the formative cells may be involved in the maintenance of their functional integrity.", "subitem_description_type": "Abstract"}]}, "item_5_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "14", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u5927\u5cf6, \u52c7\u4eba"}]}]}, "item_5_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_5_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1016/j.archoralbio.2005.09.007", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_15": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Elsevier Ltd"}]}, "item_5_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_5_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00548457", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00039969", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nakasone, Naohiro"}], "nameIdentifiers": [{"nameIdentifier": "4235", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshie, Hiromasa"}], "nameIdentifiers": [{"nameIdentifier": "4236", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohshima, Hayato"}], "nameIdentifiers": [{"nameIdentifier": "14", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "9_0002.pdf", "filesize": [{"value": "107.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 107100.0, "url": {"label": "9_0002.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/1549/files/9_0002.pdf"}, "version_id": "36d2c712-96c5-4af6-b73f-b4fdeefee112"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Cell Proliferation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cell Differentiation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Heat-Shock Proteins", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Immunohistochemistry", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Molar", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Odontogenesis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Rats", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars"}]}, "item_type_id": "5", "owner": "1", "path": ["453/454", "432/477/478"], "permalink_uri": "http://hdl.handle.net/10191/1804", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2007-05-10"}, "publish_date": "2007-05-10", "publish_status": "0", "recid": "1549", "relation": {}, "relation_version_is_last": true, "title": ["An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars"], "weko_shared_id": 2}