{"created":"2021-03-01T06:05:13.196299+00:00","id":1549,"links":{},"metadata":{"_buckets":{"deposit":"57d3e950-aa94-4367-9f8a-13f36d0dc88f"},"_deposit":{"id":"1549","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1549"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00001549","sets":["432:477:478","453:454"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"386","bibliographicPageStart":"378","bibliographicVolumeNumber":"51","bibliographic_titles":[{"bibliographic_title":"Archives of Oral Biology"},{"bibliographic_title":"Archives of Oral Biology","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objectives: The aim of this study is to clarify the functional significance of heat-shock protein (HSP)-25 during tooth development. Design: We compared the expression of HSP-25 in the dental epithelial and mesenchymal cells with their proliferative activity during odontogenesis in rat molars on postnatal Days 1–100 by immunohistochemistry using anti-HSP-25 and anti-5-bromo-2’-deoxyuridine (BrdU) for cell proliferation assay. Results: On Day one, BrdU-immunoreactive cells were densely located in the inner enamel epithelium in the cervical loop and inter-cusped areas and the dental pulp adjacent to them, whereas HSP-25-immunoractivity (IR) was restricted to the cusped area where odontoblasts and ameloblasts had already differentiated. Subsequently, BrdU-IR shifted in the apical direction to be localized around Hertwig’s epithelial root sheath during Days 5–30, never overlapping with concomitantly apically-shifted HSP-25-IR. On Days 60–100, BrdU-immunoreactive cells were hardly recognizable in the dental pulp, where HSP-25-IR was exclusively localized in the odontoblast layer. Furthermore, the odontoblast- and ameloblast-lineage cells exhibited two steps in the expression of HSP-25 throughout the postnatal stages: first, dental epithelial and pulpal mesenchymal cells showed a weak IR for HSP-25 after the cessation of their proliferative activity, and subsequently odontoblasts and ameloblasts consistently expressed an intense HSP-25-IR. Conclusion: Odontoblast- and ameloblast-lineage cells acquire HSP-25-IR after they complete their cell division, suggesting that this protein acts as a switch between cell proliferation and differentiation during tooth development. The consistent expression of HSP-25-IR in the formative cells may be involved in the maintenance of their functional integrity.","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"14","nameIdentifierScheme":"WEKO"}],"names":[{"name":"大島, 勇人"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.archoralbio.2005.09.007","subitem_relation_type_select":"DOI"}}]},"item_5_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Elsevier Ltd"}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00548457","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00039969","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakasone, Naohiro"}],"nameIdentifiers":[{"nameIdentifier":"4235","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshie, Hiromasa"}],"nameIdentifiers":[{"nameIdentifier":"4236","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohshima, Hayato"}],"nameIdentifiers":[{"nameIdentifier":"14","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"9_0002.pdf","filesize":[{"value":"107.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"9_0002.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/1549/files/9_0002.pdf"},"version_id":"36d2c712-96c5-4af6-b73f-b4fdeefee112"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cell Proliferation","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Differentiation","subitem_subject_scheme":"Other"},{"subitem_subject":"Heat-Shock Proteins","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunohistochemistry","subitem_subject_scheme":"Other"},{"subitem_subject":"Molar","subitem_subject_scheme":"Other"},{"subitem_subject":"Odontogenesis","subitem_subject_scheme":"Other"},{"subitem_subject":"Rats","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars"}]},"item_type_id":"5","owner":"1","path":["454","478"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-05-10"},"publish_date":"2007-05-10","publish_status":"0","recid":"1549","relation_version_is_last":true,"title":["An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:34:09.565349+00:00"}