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糸球体上皮細胞の培養をめざして
http://hdl.handle.net/10191/49188
http://hdl.handle.net/10191/4918805582ca7-a036-413c-b9d7-bfe606f097e1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
114(10)_369-372.pdf (880.4 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-11-21 | |||||
| タイトル | ||||||
| タイトル | 糸球体上皮細胞の培養をめざして | |||||
| タイトル | ||||||
| タイトル | 糸球体上皮細胞の培養をめざして | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | kidney | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | glomerular epithelial cells | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | podocytes | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | culture | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 腎臓 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 糸球体上皮細胞 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | タコ足細胞 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 培養 | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| その他のタイトル | ||||||
| その他のタイトル | Glomerular Epithelial Cell Culture | |||||
| 著者 |
矢尾坂, 永信
× 矢尾坂, 永信 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 97543 | |||||
| 姓名 | Yaoita, Eishin | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Glomerular epithelial cells (GECs) play crucial roles in morphogenesis and repair of the glomerulus. Injury to GECs may be the starting point for eventual glomerular tuft destruction. In spite of the biological and pathological significance of GECs, the unanimous culture system of GECs has not been established. Although cobblestone-like polygonal cells have been described as cultured GECs, their origin remains controversial. GECs are damaged during the isolation of glomaruli by the conventional sieving method, and cellular outgrowth from glomeruli devoid of Bowman's capsule (decapsulated glomeruli) has been too limited for definitive characterization of cell types. Therefore, we devised an isolation method that does not use mechanical force. The result produced substantial numbers of outgrowths from decapsulated glomeruli. Morphologically, these cells were distinctly different from cobblestone-like polygonal cells. Cells growing from decapsulated glomeruli showed intense staining for podocyte-specific markers (podocalyxin), but no staining for markers specific to mesangial cells (Thy-1), endothelial cells (RECA-1), indicating that these cells were of GEC origin. In addition, some phenotypes specific to GECs in vivo were expressed in cultured parietal epithelial cells of Bowman's capsule and tubular epithelial cells. These findings indicate that valid conclusions about experimental outcomes depend on clearcut definition of the cell types involved. | |||||
| 書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 114, 号 10, p. 369-372, 発行日 2000-10 |
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| 出版者 | ||||||
| 出版者 | 新潟医学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00290440 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00182415 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
