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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 02 新潟医学会雑誌
  4. 第114巻第10号

糸球体上皮細胞の培養をめざして

http://hdl.handle.net/10191/49188
http://hdl.handle.net/10191/49188
05582ca7-a036-413c-b9d7-bfe606f097e1
名前 / ファイル ライセンス アクション
114(10)_369-372.pdf 114(10)_369-372.pdf (880.4 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2017-11-21
タイトル
タイトル 糸球体上皮細胞の培養をめざして
タイトル
言語 en
タイトル 糸球体上皮細胞の培養をめざして
言語
言語 jpn
キーワード
主題Scheme Other
主題 kidney
キーワード
主題Scheme Other
主題 glomerular epithelial cells
キーワード
主題Scheme Other
主題 podocytes
キーワード
主題Scheme Other
主題 culture
キーワード
主題Scheme Other
主題 腎臓
キーワード
主題Scheme Other
主題 糸球体上皮細胞
キーワード
主題Scheme Other
主題 タコ足細胞
キーワード
主題Scheme Other
主題 培養
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Glomerular Epithelial Cell Culture
著者 矢尾坂, 永信

× 矢尾坂, 永信

WEKO 97542

矢尾坂, 永信

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著者別名
識別子 97543
識別子Scheme WEKO
姓名 Yaoita, Eishin
抄録
内容記述タイプ Abstract
内容記述 Glomerular epithelial cells (GECs) play crucial roles in morphogenesis and repair of the glomerulus. Injury to GECs may be the starting point for eventual glomerular tuft destruction. In spite of the biological and pathological significance of GECs, the unanimous culture system of GECs has not been established. Although cobblestone-like polygonal cells have been described as cultured GECs, their origin remains controversial. GECs are damaged during the isolation of glomaruli by the conventional sieving method, and cellular outgrowth from glomeruli devoid of Bowman's capsule (decapsulated glomeruli) has been too limited for definitive characterization of cell types. Therefore, we devised an isolation method that does not use mechanical force. The result produced substantial numbers of outgrowths from decapsulated glomeruli. Morphologically, these cells were distinctly different from cobblestone-like polygonal cells. Cells growing from decapsulated glomeruli showed intense staining for podocyte-specific markers (podocalyxin), but no staining for markers specific to mesangial cells (Thy-1), endothelial cells (RECA-1), indicating that these cells were of GEC origin. In addition, some phenotypes specific to GECs in vivo were expressed in cultured parietal epithelial cells of Bowman's capsule and tubular epithelial cells. These findings indicate that valid conclusions about experimental outcomes depend on clearcut definition of the cell types involved.
書誌情報 新潟医学会雑誌
en : 新潟医学会雑誌

巻 114, 号 10, p. 369-372, 発行日 2000-10
出版者
出版者 新潟医学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 00290440
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00182415
著者版フラグ
値 publisher
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