@article{oai:niigata-u.repo.nii.ac.jp:00015186,
 author = {矢尾坂, 永信},
 issue = {10},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Oct},
 note = {Glomerular epithelial cells (GECs) play crucial roles in morphogenesis and repair of the glomerulus. Injury to GECs may be the starting point for eventual glomerular tuft destruction. In spite of the biological and pathological significance of GECs, the unanimous culture system of GECs has not been established. Although cobblestone-like polygonal cells have been described as cultured GECs, their origin remains controversial. GECs are damaged during the isolation of glomaruli by the conventional sieving method, and cellular outgrowth from glomeruli devoid of Bowman's capsule (decapsulated glomeruli) has been too limited for definitive characterization of cell types. Therefore, we devised an isolation method that does not use mechanical force. The result produced substantial numbers of outgrowths from decapsulated glomeruli. Morphologically, these cells were distinctly different from cobblestone-like polygonal cells. Cells growing from decapsulated glomeruli showed intense staining for podocyte-specific markers (podocalyxin), but no staining for markers specific to mesangial cells (Thy-1), endothelial cells (RECA-1), indicating that these cells were of GEC origin. In addition, some phenotypes specific to GECs in vivo were expressed in cultured parietal epithelial cells of Bowman's capsule and tubular epithelial cells. These findings indicate that valid conclusions about experimental outcomes depend on clearcut definition of the cell types involved.},
 pages = {369--372},
 title = {糸球体上皮細胞の培養をめざして},
 volume = {114},
 year = {2000}
}