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ヒト多発筋炎発症機構の研究 : 樹状細胞移植による実験的アレルギー性筋炎モデルマウスの解析をもとに
http://hdl.handle.net/10191/3973
http://hdl.handle.net/10191/3973d5f9bc3c-d05e-444c-8097-d3f9f9797cb3
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-05-10 | |||||
タイトル | ||||||
タイトル | ヒト多発筋炎発症機構の研究 : 樹状細胞移植による実験的アレルギー性筋炎モデルマウスの解析をもとに | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | ヒト多発筋炎発症機構の研究 : 樹状細胞移植による実験的アレルギー性筋炎モデルマウスの解析をもとに | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Polymyositis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dendritic cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MHC-binding anchor motif | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | T cell epitope | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cross-priming | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Mechanism of Autoimmunity in Human Polymyositis, Based on the Experimental Allergic Myositis Induced by Inoculating Dendritic Cells | |||||
著者 |
河内, 泉
× 河内, 泉 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Polymyositis(PM) is an inflammatory muscle disease caused by autoimmune dysfunction, and considered to be caused by cytotoxic CD 8 T cells. To date no autoantigens have been identified. We attempted to induce an experimental allergic myositis (EAM) in BALB/c mice by inoculating syngeneic dendritic cells (DCs) presenting peptides that are expected to match the binding anchor motif of H-2Kd(BALB/c). We selected peptides that are abundantly expressed in skeletal muscle as the candidate antigens. Only when we inoculated syngeneic bone-marrow-derived DCs presenting pyruvate kinase M1/M2 peptide 464-472 in BALB/c mice, 41.7% of the mice (EAM) developed pathological changes in skeletal muscle compatible to human-PM. Under other conditions (when we inoculated DCs presenting sodium channel protein (skeletal muscle alpha-subunit) peptide 1264-1274 into BALB/c or C57BL mice, or DCs presenting pyruvate kinase M1/M2 peptide into C57BL/6 mice, there were no necrotizing and inflammatory lesions. Induction of EAM in the same manner as above also induced CTL activity against P815 cells with the pyruvate kinase M1/M2 peptide and syngeneic differentiated-cultured-myotubes without peptides by the chromium release assay. Consistent with the similarity of the binding anchor motifs of H-2Kd (BALB/c) and HLA A*2402, we found that pyruvate kinase M1/M2 peptide-specific and interferon γ-producing T lymphocytes existed in peripheral blood of human-PM with the HLA A*2402 allele by the enzyme linked immunospot (ELISPOT) assay. Therefore we concluded that pyruvate kinase M1/M2 peptide is a candidate autoantigen not only in BALB/c-EAM but also in human-PM with the HLA A*2402 allele. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 116, 号 11, p. 546-565, 発行日 2002-11 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |