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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.37 No.2

Characteristics of 6-Mercaptopurine-Resistant L1210 Cells Newly Developed by in vivo and in vitro Treatment with 6-Mercaptopurine

http://hdl.handle.net/10191/33539
http://hdl.handle.net/10191/33539
76e03917-c30b-4802-b61d-c1036a9a017c
名前 / ファイル ライセンス アクション
37(2)_63-70.pdf 37(2)_63-70.pdf (1.3 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-10-06
タイトル
タイトル Characteristics of 6-Mercaptopurine-Resistant L1210 Cells Newly Developed by in vivo and in vitro Treatment with 6-Mercaptopurine
タイトル
言語 en
タイトル Characteristics of 6-Mercaptopurine-Resistant L1210 Cells Newly Developed by in vivo and in vitro Treatment with 6-Mercaptopurine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Natsuno, Tetsuya

× Natsuno, Tetsuya

WEKO 53799

Natsuno, Tetsuya

Search repository
Saito, Kazuko

× Saito, Kazuko

WEKO 53800

Saito, Kazuko

Search repository
Mitsuhashi, Susumu

× Mitsuhashi, Susumu

WEKO 53801

Mitsuhashi, Susumu

Search repository
抄録
内容記述タイプ Abstract
内容記述 6-Mercaptopurine-resistant sublines of L1210 cell were obtained by in vivo and in vitro treatment with 6-mercaptopurine. These 27 sublines were classified into high- and low-resistant sublines. The MAC level of the high-resistance group was 0.3 μg/ml, and that of the low-resistance group was 0.3-1 μg/ml. The sensitive parental cells showed a MAC of 0.003 μg/ml. Most of the resistant sublines kept their MAC levels stable even after 9 months of serial subcultures in a drug-free medium, though some sublines changed their MAC levels. The level of [^<14>C] 6-MP incorporation by high-resistant cells was the lowest among three types of subline, that of the sensitive cells was the highest, and that of the low resistant cells was intermediate. The thioinosinate level of the sublines was estimated by Dowex-1 column chromatography. More thioinosinate was found in cell-free lysate of the resistant subline than in the sensitive subline. Activity of hypoxanthine guanine phosphorybosyl-transferase in cell-free lysate was also estimated. These sublines showed the same level of enzyme activity in contrast to the difference in the thioinosinate level. Both the 6-MP-resistant and cytosine-D-arabinofuranocide (ara-C)-resistant cells were examined reciprocally for incorporation of 6-MP and ara-C. Neither resistant sublines incorporated the corresponding drug, thus indicating that the 6-MP resistance mechanism is specific for purines. We conclude that the 6-MP resistance of the L1210 cell is due to a defective situation of cell membrane permeability or to an active exclusion system of metabolite.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 37, 号 2, p. 63-70, 発行日 1989-09
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
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