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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.39(Supplement)

Mechanism of Abnormal Active Oxygen Generation in Tissue Cells by Puromycin Aminonucleoside

http://hdl.handle.net/10191/33460
http://hdl.handle.net/10191/33460
7e5788de-351f-4232-b800-9936a9fbc796
名前 / ファイル ライセンス アクション
39(Suppl.)_53-62.pdf 39(Suppl.)_53-62.pdf (1.1 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-09-28
タイトル
タイトル Mechanism of Abnormal Active Oxygen Generation in Tissue Cells by Puromycin Aminonucleoside
タイトル
言語 en
タイトル Mechanism of Abnormal Active Oxygen Generation in Tissue Cells by Puromycin Aminonucleoside
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Aoyagi, Kazumasa

× Aoyagi, Kazumasa

WEKO 53545

Aoyagi, Kazumasa

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Narita, Mitsuharu

× Narita, Mitsuharu

WEKO 53546

Narita, Mitsuharu

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抄録
内容記述タイプ Abstract
内容記述 Puromycin aminonucleoside (PA) is known as a toxic substance which induces heavy proteinuria. However, the mechanism of inducing proteinuria has not been clarified. We have reported that PA increases active oxygen generation in isolated rat hepatocytes. In this paper, we investigated the mechanism of abnormally active oxygen generation in isolated rat hepatocytes. Active oxygen generation was measured by the conversion rate of creatinine to methylguanidine (MG), a radical adduct. MK-447, which is an active oxygen scavenger and affects arachidonic acid metabolism, markedly inhibited MG synthesis at 1 μM. CV6504, which completely inhibited proteinuria induced by P A, is an inhibitor of 5-lipoxygenase and thromboxane A_2 synthetase and also is an active oxygen scavenger. CV6504 at 50 μM almost completely inhibited MG synthesis increased by PA. AA861, a 5-lipoxgenase inhibitor, at 1 μM also inhibited MG synthesis increased by PA, but higher concentrations were not effective. Cyclooxygenase inhibitors, indomethacin and pyroxicam, inhibited MG synthesis increased by PA. These results suggested that the abnormally active oxygen generation by PA comes from arachidonic acid metabolism. However, thromboxane A_2 synthetase is not related to active oxygen generation in the arachidonic acid cascade. OKY-046, which is an inhibitor of thromboxane A_2 synthetase, inhibited abnormally active oxygen generation by PA. Stable thromboxane A_2 , ONO11114, had no effect on MG synthesis. An antagonist of platelet activating factor, YM-264, which decreases proteinuria induced by PA, also inhibited abnormally active oxygen generation by PA. These results suggest that abnormally active oxygen may come from the arachidonic acid cascade, but there might be other unknown mechanisms which regulate active oxygen generation in tissue cells.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 39(Supplement), p. 53-62, 発行日 1991-03
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
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