{"_buckets": {"deposit": "88c58274-331c-4587-8163-bb4828c7852b"}, "_deposit": {"id": "6578", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6578"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006578", "sets": ["456", "638"]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1991-03", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "62", "bibliographicPageStart": "53", "bibliographicVolumeNumber": "39(Supplement)", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Puromycin aminonucleoside (PA) is known as a toxic substance which induces heavy proteinuria. However, the mechanism of inducing proteinuria has not been clarified. We have reported that PA increases active oxygen generation in isolated rat hepatocytes. In this paper, we investigated the mechanism of abnormally active oxygen generation in isolated rat hepatocytes. Active oxygen generation was measured by the conversion rate of creatinine to methylguanidine (MG), a radical adduct. MK-447, which is an active oxygen scavenger and affects arachidonic acid metabolism, markedly inhibited MG synthesis at 1 μM. CV6504, which completely inhibited proteinuria induced by P A, is an inhibitor of 5-lipoxygenase and thromboxane A_2 synthetase and also is an active oxygen scavenger. CV6504 at 50 μM almost completely inhibited MG synthesis increased by PA. AA861, a 5-lipoxgenase inhibitor, at 1 μM also inhibited MG synthesis increased by PA, but higher concentrations were not effective. Cyclooxygenase inhibitors, indomethacin and pyroxicam, inhibited MG synthesis increased by PA. These results suggested that the abnormally active oxygen generation by PA comes from arachidonic acid metabolism. However, thromboxane A_2 synthetase is not related to active oxygen generation in the arachidonic acid cascade. OKY-046, which is an inhibitor of thromboxane A_2 synthetase, inhibited abnormally active oxygen generation by PA. Stable thromboxane A_2 , ONO11114, had no effect on MG synthesis. An antagonist of platelet activating factor, YM-264, which decreases proteinuria induced by PA, also inhibited abnormally active oxygen generation by PA. These results suggest that abnormally active oxygen may come from the arachidonic acid cascade, but there might be other unknown mechanisms which regulate active oxygen generation in tissue cells.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508361", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "05677734", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Aoyagi, Kazumasa"}], "nameIdentifiers": [{"nameIdentifier": "53545", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Narita, Mitsuharu"}], "nameIdentifiers": [{"nameIdentifier": "53546", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "39(Suppl.)_53-62.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "39(Suppl.)_53-62.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6578/files/39(Suppl.)_53-62.pdf"}, "version_id": "587cb912-247c-4e44-8696-d32b9c5f1885"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Mechanism of Abnormal Active Oxygen Generation in Tissue Cells by Puromycin Aminonucleoside", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Mechanism of Abnormal Active Oxygen Generation in Tissue Cells by Puromycin Aminonucleoside"}, {"subitem_title": "Mechanism of Abnormal Active Oxygen Generation in Tissue Cells by Puromycin Aminonucleoside", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "638"], "permalink_uri": "http://hdl.handle.net/10191/33460", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-09-28"}, "publish_date": "2015-09-28", "publish_status": "0", "recid": "6578", "relation": {}, "relation_version_is_last": true, "title": ["Mechanism of Abnormal Active Oxygen Generation in Tissue Cells by Puromycin Aminonucleoside"], "weko_shared_id": null}