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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.43 No.4

Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method

http://hdl.handle.net/10191/33220
http://hdl.handle.net/10191/33220
f199602c-67ba-4c82-a23b-e0376d72fc99
名前 / ファイル ライセンス アクション
43(4)_217-222.pdf 43(4)_217-222.pdf (644.6 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-08-31
タイトル
タイトル Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method
タイトル
言語 en
タイトル Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method
言語
言語 eng
キーワード
主題Scheme Other
主題 retrovirus vectors
キーワード
主題Scheme Other
主題 spin transduction
キーワード
主題Scheme Other
主題 Neo^R gene
キーワード
主題Scheme Other
主題 hematopoietic stem cells
キーワード
主題Scheme Other
主題 PCR
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Moriyama, Yoshiaki

× Moriyama, Yoshiaki

WEKO 53029

Moriyama, Yoshiaki

Search repository
Takahashi, Masahiro

× Takahashi, Masahiro

WEKO 53030

Takahashi, Masahiro

Search repository
Masuko, Masayoshi

× Masuko, Masayoshi

WEKO 53031

Masuko, Masayoshi

Search repository
Kishi, Kenji

× Kishi, Kenji

WEKO 53032

Kishi, Kenji

Search repository
Shibata, Akira

× Shibata, Akira

WEKO 53033

Shibata, Akira

Search repository
抄録
内容記述タイプ Abstract
内容記述 In order to improve the efficiency of gene transduction into human hematopoietic stem cells by retrovirus vectors, we conducted an in vitro study to determine the optimal conditions. Cells employed as the target were K562, a human myeloblastoid cell line. Retrovirus vectors used were LNL6 and GINa40, both carrying the Neo^R (Neomycin-resistant) gene as a genetic marker. LNL6 was provided by Genetic Therapy Inc. (Gaithersburg, MD, U.S.A.) as culture supernatants of the producing cells; GINa40 was of the supernatants alike and their concentrates from the same Inc. Transduction efficiency varied from 1.0% to 59.1% depending upon the factors and procedures in the experiments, which comprised MOI (multiplicity of infection) values, the duration of exposure of cells to the vectors, and conduct/non-conduct of centrifuge of cells during the exposure. An optimal transduction was achieved by daily supplementation up to three days of the vectors to cells, together with a centrifuge of the cells at 2,500 rpm for 90 min during their exposure to the vectors. Along with the Neo^R gene transduction, a new assay system was introduced as a related matter of importance.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 43, 号 4, p. 217-222, 発行日 1995-12
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
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