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Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method
http://hdl.handle.net/10191/33220
http://hdl.handle.net/10191/33220f199602c-67ba-4c82-a23b-e0376d72fc99
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
43(4)_217-222.pdf (644.6 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-08-31 | |||||
| タイトル | ||||||
| タイトル | Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method | |||||
| タイトル | ||||||
| タイトル | Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | retrovirus vectors | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | spin transduction | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Neo^R gene | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | hematopoietic stem cells | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PCR | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Moriyama, Yoshiaki
× Moriyama, Yoshiaki× Takahashi, Masahiro× Masuko, Masayoshi× Kishi, Kenji× Shibata, Akira |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In order to improve the efficiency of gene transduction into human hematopoietic stem cells by retrovirus vectors, we conducted an in vitro study to determine the optimal conditions. Cells employed as the target were K562, a human myeloblastoid cell line. Retrovirus vectors used were LNL6 and GINa40, both carrying the Neo^R (Neomycin-resistant) gene as a genetic marker. LNL6 was provided by Genetic Therapy Inc. (Gaithersburg, MD, U.S.A.) as culture supernatants of the producing cells; GINa40 was of the supernatants alike and their concentrates from the same Inc. Transduction efficiency varied from 1.0% to 59.1% depending upon the factors and procedures in the experiments, which comprised MOI (multiplicity of infection) values, the duration of exposure of cells to the vectors, and conduct/non-conduct of centrifuge of cells during the exposure. An optimal transduction was achieved by daily supplementation up to three days of the vectors to cells, together with a centrifuge of the cells at 2,500 rpm for 90 min during their exposure to the vectors. Along with the Neo^R gene transduction, a new assay system was introduced as a related matter of importance. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 43, 号 4, p. 217-222, 発行日 1995-12 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
