{"_buckets": {"deposit": "37891a11-8af4-4cc7-8581-980ca792111c"}, "_deposit": {"id": "6457", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6457"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006457"}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1995-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "222", "bibliographicPageStart": "217", "bibliographicVolumeNumber": "43", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "In order to improve the efficiency of gene transduction into human hematopoietic stem cells by retrovirus vectors, we conducted an in vitro study to determine the optimal conditions. Cells employed as the target were K562, a human myeloblastoid cell line. Retrovirus vectors used were LNL6 and GINa40, both carrying the Neo^R (Neomycin-resistant) gene as a genetic marker. LNL6 was provided by Genetic Therapy Inc. (Gaithersburg, MD, U.S.A.) as culture supernatants of the producing cells; GINa40 was of the supernatants alike and their concentrates from the same Inc. Transduction efficiency varied from 1.0% to 59.1% depending upon the factors and procedures in the experiments, which comprised MOI (multiplicity of infection) values, the duration of exposure of cells to the vectors, and conduct/non-conduct of centrifuge of cells during the exposure. An optimal transduction was achieved by daily supplementation up to three days of the vectors to cells, together with a centrifuge of the cells at 2,500 rpm for 90 min during their exposure to the vectors. Along with the Neo^R gene transduction, a new assay system was introduced as a related matter of importance.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508361", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "05677734", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Moriyama, Yoshiaki"}], "nameIdentifiers": [{"nameIdentifier": "53029", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takahashi, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "53030", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Masuko, Masayoshi"}], "nameIdentifiers": [{"nameIdentifier": "53031", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kishi, Kenji"}], "nameIdentifiers": [{"nameIdentifier": "53032", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibata, Akira"}], "nameIdentifiers": [{"nameIdentifier": "53033", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "43(4)_217-222.pdf", "filesize": [{"value": "644.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 644600.0, "url": {"label": "43(4)_217-222.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6457/files/43(4)_217-222.pdf"}, "version_id": "9fe09b45-6aa3-4995-b2ab-0f6a0f84ec0f"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "retrovirus vectors", "subitem_subject_scheme": "Other"}, {"subitem_subject": "spin transduction", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Neo^R gene", "subitem_subject_scheme": "Other"}, {"subitem_subject": "hematopoietic stem cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PCR", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method"}, {"subitem_title": "Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/568/621"], "permalink_uri": "http://hdl.handle.net/10191/33220", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-08-31"}, "publish_date": "2015-08-31", "publish_status": "0", "recid": "6457", "relation": {}, "relation_version_is_last": true, "title": ["Augmentation of Gene Transfer Efficiency into Human Hematopoietic Stem Cells by the Spin Transduction Method"], "weko_shared_id": null}