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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.45 No.1

Purification and Monolayer Cell Culture of Murine Extrahepatic Biliary Epithelial Cells

http://hdl.handle.net/10191/33114
http://hdl.handle.net/10191/33114
45754074-b175-4430-8f7b-f94e552c3358
名前 / ファイル ライセンス アクション
45(1)_15-20.pdf 45(1)_15-20.pdf (2.2 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-08-25
タイトル
タイトル Purification and Monolayer Cell Culture of Murine Extrahepatic Biliary Epithelial Cells
タイトル
言語 en
タイトル Purification and Monolayer Cell Culture of Murine Extrahepatic Biliary Epithelial Cells
言語
言語 eng
キーワード
主題Scheme Other
主題 biliary epithelium
キーワード
主題Scheme Other
主題 collagen gel culture
キーワード
主題Scheme Other
主題 cell culture
キーワード
主題Scheme Other
主題 tissue culture
キーワード
主題Scheme Other
主題 purification
キーワード
主題Scheme Other
主題 explant culture
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Saito, Katsuhiko

× Saito, Katsuhiko

WEKO 52787

Saito, Katsuhiko

Search repository
Katayanagi, Kazuyoshi

× Katayanagi, Kazuyoshi

WEKO 52788

Katayanagi, Kazuyoshi

Search repository
Tsuneyama, Koichi

× Tsuneyama, Koichi

WEKO 52789

Tsuneyama, Koichi

Search repository
抄録
内容記述タイプ Abstract
内容記述 The preparation of biliary epithelial cells is fundamental for their biologic and pathophysiologic evaluation. A new method for the isolation of extrahepatic biliary epithelial cells, using explants of murine extrahepatic bile ducts and gallbladder, is presented. First, small tissue pieces of the extrahepatic biliary tree and the gallbladder were plated as primary explants on collagen gel. The epithelial cells spread from the explants toward the periphery as a monolayer sheet, while the nonepithelial mesenchymal cells of the explants migrated into the gel below the epithelial layer. These different growth patterns created areas in epithelial cell sheets in which no mesenchymal cells were present. These areas were cut with scissors and then cultured on another collagen gel as secondary explants. From these explants, epithelial cells without nonepithelial mesenchymal cells spread out as a monolayer on the collagen gel. At the front and periphery of both the primary and secondary monolayer cell cultures, epithelial cells exhibited a polygonal flattened morphology, and BrdUpositive S-phase cells were densely distributed. In the central parts of both explants, the cells were cuboidal or low columnar with a luminal mucin positive layer and BrdU-positive S-phase cells were sparsely distributed, suggesting that the in vivo morphologic characteristics of the epithelial cells of the extrahepatic biliary tree were retained. This method provides purification and a monolayer cell culture or extrahepatic biliary epithelial cells that can be employed in various types of studies.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 45, 号 1, p. 15-20, 発行日 1997-03
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
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