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Two-color Flow Cytometric Analysis of Cytokine Receptors and Surface Markers on Blood Cells
http://hdl.handle.net/10191/33118
http://hdl.handle.net/10191/33118ea99c48c-80ed-4fe4-b84c-c367dc9e055f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
45(2)_39-44.pdf (731.7 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2015-08-25 | |||||
| タイトル | ||||||
| タイトル | Two-color Flow Cytometric Analysis of Cytokine Receptors and Surface Markers on Blood Cells | |||||
| タイトル | ||||||
| タイトル | Two-color Flow Cytometric Analysis of Cytokine Receptors and Surface Markers on Blood Cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | two-color flow cytometry | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cytokine receptors | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | fluorescence labeled cytokines | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | surface markers | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Maruyama, Soichi
× Maruyama, Soichi× Aoki, Sadao× Tsukada, Nobuhiro× Nomoto, Nobuhiko× Aizawa, Yoshifusa× Takahashi, Hoyu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In order to analyze the expression of cytokine receptors on a specified cell population, two-color flow cytometric analysis of cytokine receptors and surface markers using fluorescence labeled cytokines and monoclonal antibodies was performed. In normal peripheral blood cells, granulocytes expressed granulocyte colony-stimulating factor (G-CSF) receptor and interleukin-6 (lL-6) receptor, but mostly expressed interleukin-3 (lL-3) receptor weakly. Monocytes were positive for each receptor. In lymphocytes, CD3 positive T cells did not express any of these receptors; however, CD19 positive B cells expressed G-CSF receptor, IL-3 receptor, and partly expressed IL-6 receptor. In clinical cases, leukemic cells of acute myeloid leukemia (AML) patients tended to show the same characteristics as normal granulocytes and, in common acute lymphoblastic leukemia (ALL), B cell type chronic lymphocytic leukemia (CLL), leukemic cells tended to show the same characteristics as normal B cells. This shows that G-CSF receptors are expressed not only on myeloid cells but also on normal and leukemic B cells. Results show that two-color flow cytometry accompanied with surface marker analysis allows for the easy analysis of the expression of cytokine receptors on the specified cell population and the obtaining of useful information for clinical cytokine therapy. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 45, 号 2, p. 39-44, 発行日 1997-06 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
