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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.45 No.2

Two-color Flow Cytometric Analysis of Cytokine Receptors and Surface Markers on Blood Cells

http://hdl.handle.net/10191/33118
http://hdl.handle.net/10191/33118
ea99c48c-80ed-4fe4-b84c-c367dc9e055f
名前 / ファイル ライセンス アクション
45(2)_39-44.pdf 45(2)_39-44.pdf (731.7 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-08-25
タイトル
タイトル Two-color Flow Cytometric Analysis of Cytokine Receptors and Surface Markers on Blood Cells
タイトル
言語 en
タイトル Two-color Flow Cytometric Analysis of Cytokine Receptors and Surface Markers on Blood Cells
言語
言語 eng
キーワード
主題Scheme Other
主題 two-color flow cytometry
キーワード
主題Scheme Other
主題 cytokine receptors
キーワード
主題Scheme Other
主題 fluorescence labeled cytokines
キーワード
主題Scheme Other
主題 surface markers
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Maruyama, Soichi

× Maruyama, Soichi

WEKO 52731

Maruyama, Soichi

Search repository
Aoki, Sadao

× Aoki, Sadao

WEKO 52732

Aoki, Sadao

Search repository
Tsukada, Nobuhiro

× Tsukada, Nobuhiro

WEKO 52733

Tsukada, Nobuhiro

Search repository
Nomoto, Nobuhiko

× Nomoto, Nobuhiko

WEKO 52734

Nomoto, Nobuhiko

Search repository
Aizawa, Yoshifusa

× Aizawa, Yoshifusa

WEKO 52735

Aizawa, Yoshifusa

Search repository
Takahashi, Hoyu

× Takahashi, Hoyu

WEKO 52736

Takahashi, Hoyu

Search repository
抄録
内容記述タイプ Abstract
内容記述 In order to analyze the expression of cytokine receptors on a specified cell population, two-color flow cytometric analysis of cytokine receptors and surface markers using fluorescence labeled cytokines and monoclonal antibodies was performed. In normal peripheral blood cells, granulocytes expressed granulocyte colony-stimulating factor (G-CSF) receptor and interleukin-6 (lL-6) receptor, but mostly expressed interleukin-3 (lL-3) receptor weakly. Monocytes were positive for each receptor. In lymphocytes, CD3 positive T cells did not express any of these receptors; however, CD19 positive B cells expressed G-CSF receptor, IL-3 receptor, and partly expressed IL-6 receptor. In clinical cases, leukemic cells of acute myeloid leukemia (AML) patients tended to show the same characteristics as normal granulocytes and, in common acute lymphoblastic leukemia (ALL), B cell type chronic lymphocytic leukemia (CLL), leukemic cells tended to show the same characteristics as normal B cells. This shows that G-CSF receptors are expressed not only on myeloid cells but also on normal and leukemic B cells. Results show that two-color flow cytometry accompanied with surface marker analysis allows for the easy analysis of the expression of cytokine receptors on the specified cell population and the obtaining of useful information for clinical cytokine therapy.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 45, 号 2, p. 39-44, 発行日 1997-06
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
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