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Screening Downstream Genes of a Homeobox Gene by Differential Display Using a Knockout Mouse
http://hdl.handle.net/10191/33080
http://hdl.handle.net/10191/33080892d310c-ba4d-4652-ac42-421411d182c0
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
46(2)_63-70.pdf (1.3 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-08-21 | |||||
| タイトル | ||||||
| タイトル | Screening Downstream Genes of a Homeobox Gene by Differential Display Using a Knockout Mouse | |||||
| タイトル | ||||||
| タイトル | Screening Downstream Genes of a Homeobox Gene by Differential Display Using a Knockout Mouse | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | homeobox gene | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | downstream genes | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | differential display | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Msx1 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | knockout mice | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Nishizawa, Kazumichi
× Nishizawa, Kazumichi× Satokata, Ichiro× Kawano, Yoshiro× Uchiyama, Makoto |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The products of homeobox genes are DNAbinding transcription factors. However, little is known about downstream genes whose activities are regulated directly or indirectly by the homeobox genes. In the current study, we tested a differential display (DD) method using the tissue of a knockout mouse in order to identify the downstream genes of a homeobox gene Msx1 systematically. Our previous in situ hybridization analysis of a Msx1 deficient mouse showed that Msx1 induced by epithelial bone morphogenetic protein 4 (BMP4) and fibroblast growth factors (FGFs) induces Bmp4, the HMG box gene Lef1, and the heparan sulfate proteoglycan syndecan-1 in the tooth mesenchyme. Although it is a very powerful approach for identifying downstream genes of a homeobox gene to test whether the candidate gene's expression is affected in the knockout mouse, this approach is not directly applicable to the identification of unknown genes downstream of Msx1. In the current study, we performed DD using total RNA from E14.5 Msx1 mutant mandibles and were able to obtain four novel downstream genes of Msx1 from 20 cDNA clones verified by Northern blot hybridization and semiquantitative RT-PCR. Despite several problems inherent to this method, we concluded that DD analysis using the tissue of a knockout mouse is a useful systematic approach for the identification of downstream genes of a homeobox gene. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 46, 号 2, p. 63-70, 発行日 1998-06 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
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Cite as
Nishizawa, Kazumichi, Satokata, Ichiro, Kawano, Yoshiro, Uchiyama, Makoto, 1998, Screening Downstream Genes of a Homeobox Gene by Differential Display Using a Knockout Mouse: Niigata University School of Medicine, 63–70 p.
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