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In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis
http://hdl.handle.net/10191/1839
http://hdl.handle.net/10191/1839dc1dd3de-b372-40d0-b422-9e06ea7f7b59
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000007215.pdf (2.5 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-05-10 | |||||
| タイトル | ||||||
| タイトル | In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis | |||||
| タイトル | ||||||
| タイトル | In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | porcine hepatocytes | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | antibody-mediated cytotoxicity | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | MTT assay | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microencapsulation | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
CAPOOR, AK
× CAPOOR, AK× KHAN, AA× HABIBULLAH, CM |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Xenotransplantation of porcine organs to untreated primates results in hyperacute rejection involving natural antibodies and complement. These antibodies rapidly interact with the vascular endothelium of discordant xenograft, activate the complement, and cause destruction of the vascular endothelium. The present study investigated the efficacy of two modalities to overcome the above barrier. The study was carried out on non-treated porcine hepatocytes, UV-B treated hepatocytes, and encapsulated hepatocytes which were incubated with normal human AB serum and complement-inactivated serum. Antibodymediated cytotoxicity to porcine hepatocytes was assessed by MTT (tetrazolium) assay. Complement inactivation of normal human AB serum resulted in significantly less cell lysis in comparison with normal human serum. Non-irradiated and UV-B irradiated hepatocytes did not show any difference, indicating that surface-antigenic epitopes are not altered by UV-B irradiation. Microencapsulation of hepatocytes in alginate poly-L-lysine capsules, however, provided protection against antibody-mediated cell lysis, suggesting that this immunoisolation technique may be useful in xenotransplantation. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 47, 号 3, p. 97-102, 発行日 1999-09 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
