ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "7497e465-eb7d-4b09-ab0e-97e69b545e8a"}, "_deposit": {"id": "6346", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6346"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006346", "sets": ["456", "606"]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "102", "bibliographicPageStart": "97", "bibliographicVolumeNumber": "47", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Xenotransplantation of porcine organs to untreated primates results in hyperacute rejection involving natural antibodies and complement. These antibodies rapidly interact with the vascular endothelium of discordant xenograft, activate the complement, and cause destruction of the vascular endothelium. The present study investigated the efficacy of two modalities to overcome the above barrier. The study was carried out on non-treated porcine hepatocytes, UV-B treated hepatocytes, and encapsulated hepatocytes which were incubated with normal human AB serum and complement-inactivated serum. Antibodymediated cytotoxicity to porcine hepatocytes was assessed by MTT (tetrazolium) assay. Complement inactivation of normal human AB serum resulted in significantly less cell lysis in comparison with normal human serum. Non-irradiated and UV-B irradiated hepatocytes did not show any difference, indicating that surface-antigenic epitopes are not altered by UV-B irradiation. Microencapsulation of hepatocytes in alginate poly-L-lysine capsules, however, provided protection against antibody-mediated cell lysis, suggesting that this immunoisolation technique may be useful in xenotransplantation.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508361", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "05677734", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "CAPOOR, AK"}], "nameIdentifiers": [{"nameIdentifier": "52481", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KHAN, AA"}], "nameIdentifiers": [{"nameIdentifier": "52482", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HABIBULLAH, CM"}], "nameIdentifiers": [{"nameIdentifier": "52483", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00000007215.pdf", "filesize": [{"value": "2.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2500000.0, "url": {"label": "KJ00000007215.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6346/files/KJ00000007215.pdf"}, "version_id": "10225287-9a05-4a0e-a545-2fe22e0711cc"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "porcine hepatocytes", "subitem_subject_scheme": "Other"}, {"subitem_subject": "antibody-mediated cytotoxicity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "MTT assay", "subitem_subject_scheme": "Other"}, {"subitem_subject": "microencapsulation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis"}, {"subitem_title": "In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "606"], "permalink_uri": "http://hdl.handle.net/10191/1839", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-05-10"}, "publish_date": "2007-05-10", "publish_status": "0", "recid": "6346", "relation": {}, "relation_version_is_last": true, "title": ["In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis"], "weko_shared_id": null}
  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.47 No.3

In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis

http://hdl.handle.net/10191/1839
http://hdl.handle.net/10191/1839
dc1dd3de-b372-40d0-b422-9e06ea7f7b59
名前 / ファイル ライセンス アクション
KJ00000007215.pdf KJ00000007215.pdf (2.5 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2007-05-10
タイトル
タイトル In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis
タイトル
言語 en
タイトル In Vitro Assessment of Microencapsulation in Providing Immunoprotection Against Antibody-Mediated Cell Lysis
言語
言語 eng
キーワード
主題Scheme Other
主題 porcine hepatocytes
キーワード
主題Scheme Other
主題 antibody-mediated cytotoxicity
キーワード
主題Scheme Other
主題 MTT assay
キーワード
主題Scheme Other
主題 microencapsulation
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 CAPOOR, AK

× CAPOOR, AK

WEKO 52481

CAPOOR, AK

Search repository
KHAN, AA

× KHAN, AA

WEKO 52482

KHAN, AA

Search repository
HABIBULLAH, CM

× HABIBULLAH, CM

WEKO 52483

HABIBULLAH, CM

Search repository
抄録
内容記述タイプ Abstract
内容記述 Xenotransplantation of porcine organs to untreated primates results in hyperacute rejection involving natural antibodies and complement. These antibodies rapidly interact with the vascular endothelium of discordant xenograft, activate the complement, and cause destruction of the vascular endothelium. The present study investigated the efficacy of two modalities to overcome the above barrier. The study was carried out on non-treated porcine hepatocytes, UV-B treated hepatocytes, and encapsulated hepatocytes which were incubated with normal human AB serum and complement-inactivated serum. Antibodymediated cytotoxicity to porcine hepatocytes was assessed by MTT (tetrazolium) assay. Complement inactivation of normal human AB serum resulted in significantly less cell lysis in comparison with normal human serum. Non-irradiated and UV-B irradiated hepatocytes did not show any difference, indicating that surface-antigenic epitopes are not altered by UV-B irradiation. Microencapsulation of hepatocytes in alginate poly-L-lysine capsules, however, provided protection against antibody-mediated cell lysis, suggesting that this immunoisolation technique may be useful in xenotransplantation.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 47, 号 3, p. 97-102, 発行日 1999-09
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 19:40:59.291749
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3