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Characterization of Low-and High-level Adriamycin-Resistant Human Gastric Cancer Cell Lines: Flow Cytometric Studies and Reversal Effect of Verapamil
http://hdl.handle.net/10191/1824
http://hdl.handle.net/10191/1824276cec1a-4800-41f2-8299-3da26c223f4d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000007229.pdf (2.5 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-05-10 | |||||
| タイトル | ||||||
| タイトル | Characterization of Low-and High-level Adriamycin-Resistant Human Gastric Cancer Cell Lines: Flow Cytometric Studies and Reversal Effect of Verapamil | |||||
| タイトル | ||||||
| タイトル | Characterization of Low-and High-level Adriamycin-Resistant Human Gastric Cancer Cell Lines: Flow Cytometric Studies and Reversal Effect of Verapamil | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | adriamycin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | drug resistance | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | verapamil | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | gastric cancer cell line | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | flow cytometry | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
UEKI, Kyo
× UEKI, Kyo× AIZAWA, Kikuo× TANAKA, Shinsuke× NISHIMAKI, Tadashi× SUZUKI, Tsutomu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have established two adriamycin (ADM)-resistant sublines with different levels of drug resistance, MKN45R0.05 and MKN45R0.8,from the parental MKN45 human gastric cancer cell line by exposure to stepwise increases in ADM. MKN45R0.05 and MKN45R0.8 cells were 4.8-and 15-fold more resistant to ADM than MKN45 cells, respectively. Flow cytometric (FCM) analysis revealed that MKN45R0.8 cells had significantly lower accumulation of cells in G_2M phase and higher efflux rate of intracellular ADM content than MKN45 and MKN45R0.05 cells. There was no significant increase of P-glycoprotein expression in both resistant sublines by FCM analysis. Verapamil (VPM) showed stronger modifying effects on ADM sensitivity and cell kinetics in MKN45R0.8 than MKN45R0.05 cells. These results indicate that FCM analysis is useful for determining the level of drug resistance, drug accumulation and P-glycoprotein expression in cancer cells, and that the differential resistance-modifying activity of VPM depends on the level of drug resistance. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 47, 号 4, p. 161-167, 発行日 1999-12 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
