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Chemical Deafferentation of the Nasal Neuroepithelium Profoundly Reduces Cholecystokinin Gene Expression in the Mouse Olfactory Bulb
http://hdl.handle.net/10191/1835
http://hdl.handle.net/10191/18355d2ba843-7a15-49ef-af45-3951c25d6bea
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-05-10 | |||||
タイトル | ||||||
タイトル | Chemical Deafferentation of the Nasal Neuroepithelium Profoundly Reduces Cholecystokinin Gene Expression in the Mouse Olfactory Bulb | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Chemical Deafferentation of the Nasal Neuroepithelium Profoundly Reduces Cholecystokinin Gene Expression in the Mouse Olfactory Bulb | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chemical deafferentation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | olfactory bulb | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CCK mRNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | in situ hybridization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tufted cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mitral cell | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
著者 |
KOZAKAI, Ikuo
× KOZAKAI, Ikuo× ITOH, Yasuhiro× KUWANO, Ryozo× AOYAGI, Yutaka× ASAKURA, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A denervation study was carried out to determine whether damage to the neuronal perikarya in the primary olfactory pathway alters the transneuronal level of cholecystokinin (CCK) mRNA expression. The time course study of denervation-induced change in CCK mRNA expression was done using northern blot and in situ hybridization methods. Northern blot analysis revealed a significant reduction in CCK mRNA expression. The quantified radioactive orbital densities in the olfactory bulb in the control at 48 hours, 7 days, and 14 days after the denervation were 1025 (100%), 743 (72%)/513 (50%), 358 (35%)/498 (49%) and 325 (32%)/338 (33%) photo-stimulated luminescence, respectively. In situ hybridization indicated that CCK expression in external tufted cells and middle tufted cells decreased time-dependently, reaching 76% of the control levels at 48 hours, 45% at 7 days, 26% at 14 days, and 15% at 30 days, respectively. CCK mRNA was expressed also in the deep tufted cells and mitral cells. Low-power darkfield photomicrography revealed that deep tufted cells and mitral cells did not undergo a profound decrease as seen in the external and middle tufted cells. These data suggest that CCK mRNA expression is differentially controlled by cell subpopulations in the olfactory bulb after deafferentation. | |||||
書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 50, 号 1, p. 53-60, 発行日 2002-03 |
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出版者 | ||||||
出版者 | Niigata University School of Medicine | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05677734 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508361 | |||||
著者版フラグ | ||||||
値 | publisher |